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CRIMIC CR01868

Gene CG13950

CG13950

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:861806..860197	21E2	FBti0213009	91337

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	short band	CTGGGAAGATGTCATTGGTTTGGACG	TTGCATTCACAGTCAGACGGTGAAGC

Images

Construct Design Sequences

sgRNA sequence	GACTTTAACCCAGATCAAAGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGGCCAAGATGGAGAACACG	AGTGGAGAACCGGGTATCCA	TCTGGGTTAAAGTCAAAGGTCA	TCCGATCGAAGTTCACGTGG

 

Amplicon 5'

Amplicon 3'

AGGCCAAGATGGAGAACACGGCTAGAAGACATTCTAGTAATAGTGGTTACTAGGAGTACTAAAATGTAGCAGTAAGATATAAAAAGGATATGAGCCACCATCAAGGAGTGCCTATTCCTCCAACTAGCACTTATACTTTCTTAATATGAATTTAAGATGACAATTACAACATATTCCTTGTATTTTTATAACTAGTAGCTTGCAAAGTTATTCGGGCAATTAATTTTATGATCGGAAATTCATTATGCGATAAGCAGTTTTTCGAGTTAGGATCATAAATGTTTTATCTTCGAAAAAGTATTACAACGCTTCCGGGTTTCCCAAGTTATGTGGTGCAACAATACTGAATATTCAATATTTAAGCAAAAATAAGGATAATAACTTATCTTTGGCTAGTTTTTCTCCCAGTGTCGCTGGTAACTAGCAACAAATGGGCAGTCGGCAGTTGGGGGCGTGACAGCAACCGTGAAAAGAACTCGAGGATGACAGTTATGGGTTACAAAGGCAAGGATTGAATCAAATAAATAAACGCGCAAGGTTCTCGTCATGGAGCGCGGCTCACTGGGTCGGTTATTAGGTGGCCAGGGGGACAGGGAGCCACCACCGAGTGGATTTATATGGGATTCGATTGAACGGGTTGAGCTCCGACCGGGGTGGGTTCGGGCATGGGCCTGGGGGTAACCTTACCACGTCCACATCCACGTCCATGTCAAGTTTGCATGCGGCTGCAGGTAATTCCCCATTTCACCCGCCTTTACATAATCACAACAAATGAACGAGGAAAGGCGACGGATGGCGAATCTATTAGCATAAGGGCGCATAAAACGGCGCCGGATCCGGCTGGATTCCATCGAGGATTCGCACTCGTCGACTCAGTTGCAGTGGAGCCGTGGAGCCGCGATGAACGTTTGGAAAGCAAAGGTCCTAACGGAGGTCCCATTCGGACACGTCCTCATCGTCAGTGGGCGCGTCAAGCCTCATCCGAATAAGTAATCATCTTTCATAGTTTGGATACCCGGTTCTCCACT

TCTGGGTTAAAGTCAAAGGTCAAATGTAGAAGCTGATTTTCTAACCAAATGAAATTAACCAAATGAAATAAACCAATTAGTTCAAAAAGTAAAAAGAGATTAAGTATAAATGGTATTCGAATTATCCGATTATTGACAAAACAATGCAGTTCTAATGCCAACGAAAGGCTTATAAAGCTGTGACAGAGATTTGCAGAAATGAAATGGGTATCCTTAGCGAGTTTGGGGGAGTGCCGAGTATCCTCCCAATCCATATCTAATACTTTTTACTAATGACACCTCTCGAATTACGGACAGAATTTCGCTGGACCTCACGGACAATGTGAACGCGCAGAACGAGAGCGAAACGGTGTTCCTGAAGATCGAGGCCAATTTCCGCGAGGGCCAAATCATCCGCAGCATGTTCCAGCCGGGCGGGGGATGGCAGCAGGAGGAGATCTCGAAGAACTGGAAGTGCGACGGCCCAAAGAACCCGCTGCAGCCTGGCCAGAGCTTCACCTTCCGGGTGGCGGTGCTGCAGCGATGCTTCGAGATCTACGTGAACGACCAGCTGTACGGGTCCTTCGAGTTCGTCAAGTTCCCCAAGCAAATCAACTACGTGCGCACCTACGGCGACTTCGAGAAGATCACCCAGTTCCACCACCGCATGCTCTTTCCGCTCGTCTTCCCCAGAACGCTCATGTGTCCGGACAAGGTGGCCTTCCAGAGCGACGTGCCCAGGCGGTATGAAACCGGCACAGTGGTGGCCATGGAGTGCATCGCCAAGGGCCCGCCGACCACGGAGTTCTCCATCTGCTTCCAGTGCAACGACACCGGGCGTACGGTGCTCCGGTTCCACGTGAACTTCGATCGGA

 

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