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CRIMIC CR01871

Gene Wbp2

CG11009

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3L:12853169..12850165	69E6	FBti0213011	91339

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCTTGGTCTTGATCACCTCCTTGAGG	TGGTCCACCTGGATAGCCATTTGGAT

Images

Construct Design Sequences

sgRNA sequence	TAAATGTAAACACTGACGTGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TATCCAGGTGGTGTGTGCAC	GACGTCAGGGATGTTCAGCA	GCTGGCGATTTGAAAGGAAATT	GGAGGTTGGACATAGCCACC

 

Amplicon 5'

Amplicon 3'

TATCCAGGTGGTGTGTGCACCAAGCCATTCAGGTATAGTTGCGTCGAATCGGGCAGAGTGAGCGAGTTGGATGTGGCCTGCAACTTGGGTTCCTTGTGCATCGTCGAGGAGCTCACATCCGCAGAGTGTTCGTTCTCATTCTCCAGATCCATGGGCGCACTGCCCTGTGGTTGTTGTTGTTGTTTTGCCATCTTATTCTGGATCTGTTTGGGGGGCGAAGAGGGATTTCCCACGACAGATGTGCTCGAGGCCGCCGTGGAAATTGCAGCTGCCAATTGGTTGAGGAAATTCGGGTGATTTGGCAAACTGACGCCATTCGGCATGGTCCCAAAGAGCGGTACTGCAAAATGTACACAGTTTGTAATTTGCTTTAAAAGAGATAACAACCCAAACGAAGCCGGTAGCAAAACAAAATAACCAATCAGCTGAGCACCTCAATTTTCGATAGGTAAAAAAAGGAAATAAAAGCTGGGGGGTGGCAAGTGCCCAAAATGGCGTAAGTCCCACCTGCTGATAAAAGTCGAATTCACCGCAAATACTGTTGTTTCTTAATTTCCCAGACCTTTCTATTTATTCCATTTTCTTTGCTACACATTTTGTGGGCTTTTTAAAATCATTATCTTATCACTTACGCATATTAACGCCCCAGTAATTGTACGACAGGTATGTATAAAAAAAAACTAATTATATTTAGAGATGAGTGACTGCTCGTACTGGTCACCCTAAAAAGAGTATCGATATCCGATAATTTCGGCGCTGCCACACTTTTCACTATGAATCATCAGCTGTCGTCAGTTTTCTAGAAAAACAAAACGTAGTTGTATGAAATTTTTGCAAATTTTTGTGGACTCAAAACAGTTGGAAAATTAAAGAGACATGTCGGTTAATACGGCTCACGCCAACAATGGCGTGCTCATCCATGCTGGAGAGTAGTAAGTTAACGGAAAAGTCCTTTAATTAACACTGAAAATGCTGAACATCCCTGACGTC

GCTGGCGATTTGAAAGGAAATTAGCCTATGTTCCAGCGATAAGATAGAAATATGGCACACAAATTAACTGCTTTTACACTTTCAGCATACTGCTCCACAGCGACAGTGTCTCCATGGAGTTCTCCGGTCAGGACAATCCCATCTTCAAGGGCTCCAAAGGTGGCAGGATTTATCTCACCTCCCACCGAATGATCTTCAATAGCAAGAAGTCCTCGGATTCGATGCAGTCGTTCAGTGCCCCGTTTGTTGCTCTTTCGGATGTGGAAATCGAACAGCCCGTTTTCGGGGCCAACTACATCAAGGGAAAGGTTCGCGCTCAGCCAAATGGAAACTACGTGGGCGAGGTCAAGTTCAAGCTCCATTTCAAGGCAGGCGGCGCAATTGAGTAAGTGCATTGTGCACCTAAATTAAAACTAATTAGACGGATCTCTTGATAAGTCTTAATACTGAATAGACTCAAGAGAATATATAATGTTCTGATTAACAAGTCCATTTCTTATAGATATGGTCAAGCTCTACTGCGCTCCGCCAAGACGGCGATGAACAACTACCACCGCGGTGGTCTGGCTGGTGATGATCCGCCACCATATCAGCCGGCTGGATCCTGGAATGAGGCACCACCGCCGGCATATCAGCCACCACCTGGCTACTATGGCTGGCTGCCGCAGCATGACGCATTCAGCGGCCCTGCGCCGAATACGGTCTATATGAGCGACAATCCGCCGCCGTATCCGGGTATCGGTAAGTTAAACGCACGTGCACTTTACCCAATCTATACTCGGCTTTTGTTTTGGCACGCTGGCTACGCGAATTGGCAACCTGGCTTACCCACGAAATTCACGCTACGCACTCGCTTCGTTTAACCCAATGGGGCACTCCTCTCTTTTTCGTCTTAGCACCACCTGCTCACCAACCAGCACCGCAACAGCCGCAGGCACCGTCGTCGAATGAACCCAATTGGTATGGTTTTTCAGCGCCGCCGCCGCAGCAGCAACAACAGCAGCCGGGCTATGGGCCACAAGGATGGGCCGGTGGCTATGTCCAACCTCC

 

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