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CRIMIC CR01873

Gene CG8560

CG8560

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3L:7402332..7404181	65F11	FBti0213440	91422

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTTCACAACGTGGCTTAGAGGGAGGA	AGCGTACAGAACGTTGGTGGAACT

Images

Construct Design Sequences

sgRNA sequence	TGTGCAAGTTACAGACTAAATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGCACTGCTGATTGGCTTTC	TGCACAAAACCTCTTAGCCAG	TAATTGCCCGTGGAACCACT	AGCAGCAGCCGAAAGTTTTG

 

Amplicon 5'

Amplicon 3'

GGCACTGCTGATTGGCTTTCTGGTCTTGCGCCACATACGGGTGGTGGTGTGAGAGTATTCATAGCCATCGGGATTCACCACCGGCAGGATAACCCAGTCAAAGTCCTTCAGCAGTTCCGAGTTGGCAGCAAAGTTCTCCACCAGCTGATGGATCACATACAGAGCTCCAGCATGGGCGATCCATTCGCGAGCATGGATACCAGCATCCAGGAAGACAACATTCTTGCCGGTCTTACCATCGCCATTGGTGATAGTGATGGTCTTGATGTCACGATTCTCATACGACTTGCCAGCCACCTGGACGGATACACGGCTGGGATAGGCAGCAGCCAGTTCATCCAGGTAGGCATTGATCTCGGCATGACGATGGAACGCCTTGAAGCTAACGCTGCGTTCAGCGGATCGCAGGTTCATCAACCTCTGGTTCTGGTTCTCATCGCGCTCCTGACGCAGCGACTCACCGAAATTCTCGTTGATCACCGAGTAGTTTACGCCGTACTTCTCCAGCAGGTGCTCGAATCCCTCCTGATCCTCGGGCTTGACCATCACGCGACTGGACGCGTCCAGGGAACGGGGCAGGTCAAAGAAGTCGTATGCCTCATTTCCGGACAAACGCAGCAGCAGCTGCTTCTCAAAAGCGTTGCGAGCATTGATGTCGTAGATCTTGTAGCTGTAGATAAAAAAAAATCCATTTTAAGTTGCTTCGAACTTATTTTTTTTTTTAGAGTTTCACGAGCTTTAAAAGTTGATATTTTTAAAATATCCTATTAAAATCAGCTTGCACCCCTTGATTTAATTGAATCACGCTGGCTAAGAGGTTTTGTGCA

TAATTGCCCGTGGAACCACTCCTCCTCCGCCGGCTCACTCACCCATCGTAGTTTTCCACCGCCAAGGCCACGCTGGCCAGCAGGAGCACCACAGCAAATAGTTTCAACATTTTTCAGTCCGCTCCGTTCCGTTTGGGCCCAGGGCTTCTAATGTGGCTCGAATACACAGATTGTGGCTTATATAGCCAACTGCCAGCTCAGCGATCATTCACAGCCCACCACCCCTTTTGGGCTTCTTATCAATGAAGAACTCTATCGGTAAAGAGCAGTTATCAGTGCGCAGATGATGGGCGATGTTCGATTTCATTTGATTTGATTTGTTGTTGTGTCTTCGCCGAGCGATGGAGAAATTCTAAAAAAAAATCTCTCAAAATTTATACTCATTGACAAGAGTATGACAGACTGGCTCGTAGTAAAAGTTTTTCACTTGACTAACTCATGACTACTCGGCAGGGTTTTAAAACATTTTGTTTTGTTACGTTAATGGCTGGAAAATAAGTTGAGTCAATGAATAAAACGCATTTAAAGTAAACCGTTTTGATTGTGAATTGCAAATTTTTAAATAAACAACTATGTGCTTGGGTCAATTTTGTATATGTCATTCACCGAAGATATCTGACATTTTTAGACGGCTTTGGAGCGACTCGTAAATTTGGTCTTCTGACTTTGCGCAATCAACATTTGATTTATATGGCCTTTTCGGCCTTAGACTCCGTCAATTCTTGGAGTGCGCCAGTCGCAGTCTCTATCTTTATTTTTTTTTTGGTATTTGAATTTGTTTGCAAAACGTGGCTTTATCAGTGTGCTCTGTGCACTGCACCAAGAAAAAAGTTTTCGGTTTAAAACAATGAAAGTCCGTACAAAAACAAAAAGAAAAACTAACGCAAAAACAAAAAAATCAAAAACAACTAACTAAAAACTCCGGCTGCCGCGCACACAAAATATATTTGAAAATTTATTGCATTTCATGAGTTTTTCCATATTTGTCTGCATTTGTAGGCAGAGTTTTGGTCACAAAACTTTCGGCTGCTGCT

 

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