CR01878

CRIMIC CR01878

Gene Hr96

CG11783

Information

Donor Location Cytology FBti Stock number
pM37-p03R:25025235..25029351 96B16-96B17Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
short band OK GAAGATAGTCGACCAGATCGTATCCG CACGTCTTTGTGTATCACTCGCGATC

Images

Construct Design Sequences

sgRNA sequence TATCTTCGTCAGCCAGTTTCTGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
AACTTGATGCACTCGCCAGA CTGGCTGACGAAGATACGGC AGCAATGCTCGCCTACCAAA TCAGTGCGTATGTTGCCCAT
 
Amplicon 5' Amplicon 3'
AACTTGATGCACTCGCCAGAGGACGCCATCGATATAATGAACAAGTTCATGAATACCCCAGCGGAGGCGCTGCGCATTCTTAACCGAATCCTAAGCGGCGGAGGAGCGAACGCAGCCCAGCAGACAGCAGACCGCAAGCCATTGCTGGACAAGGAGCCGGCGGTGAAGCCTGCAGCGCCAGCGGAGCGAGCTGATACTGTCATTCAAAGCATGCTGGGCAACAGTCCGCCAATTTCGCCACATGATGCTGCCGTGGATCTGCAGTACCACTCGCCCGGTGTCGGGGAGCAGCCCAGTACATCGAGTAGCCACCCCTTGCCTTACATAGCCAACTCGCCGGACTTCGATCTGAAGACCTTCATGCAGACCAACTACAACGACGAGCCCAGTCTGGACAGTGATTTTAGCATTAACTCAATCGAATCGGTGCTATCCGAGGTGATCCGCATTGAGTACCAGGCCTTCAATAGCATACAACAAGCGGCATCGCGCGTAAAGGAGGAGATGTCCTACGGCACTCAGTCTACGTACGGTGGATGCAATTCGGCTGCAAACAATAGCCAGCCGCACCTGCAGCAACCCATCTGCGCCCCATCCACCCAGCAGTTGGATCGCGAGCTAAACGAGGCGGAGCAAATGAAGCTGCGGGAGCTGCGACTGGCCAGCGAGGCTCTTTATGATCCCGTGGACGAGGACCTCAGCGCCCTGATGATGGGCGATGATCGCATTAAGGTAACCAACAGTCCACGGTATTAGCCTATAGGTCTTTCTACATTTATAGCTCCAACACCACGGCTTATCTAATCAGAGTGTGCGAGCTGCGATATATGTACACACGGCACCTGGCACTTTTTAGCCATTCGGTGATTCAGTGCGTCTCTCGATGTTGGCCCACGGGCCGTATCTTCGTCAGCCAG AGCAATGCTCGCCTACCAAATGTAAACACACTTTTTAATGGGGTGGCTCAAAGTTTTTGATTTCCCAAGAGCTTTGGTCGAGTAAAAGAAAATTGATCGAACCAGATAAGCTATTTTCCCCCAGAGGGTTAAAGAATTTGAAGTCATGCGACTGGGTCTAGTTAAGATATTTGATTACGAAAATTGGCCTTTAATTAAGACCCTAAACGTGACAAACTTCCATTCTATATACTTCTTGATGAGTATTTAAACAAATATGGCTATTTTCGGAACAAATCGGGCACTCATTTATATCTTTAGCTTTATCTTTATTTTTTAAGATGTGTCCACACCTTTGATCGACCTCTAGTTCCCCTGGAGAAATGATTTGGAATTATCCAATAATGATTCATCACTTCCACGAATTGTTGTCCCATTAATCGAGCCACCCTAGCTTTCATGCAATCAGAACGTCTGGTCTGCCAAGAAGGAGCAGACAGCGGCTTTATCAGCCTCTGGGCGTGCCAATTGTGACACTATCAACCATTATCAAGAGTACCAGCAGGCGCTCATGAGTCTCCAGCCAGCCGTCGATTTGGGCGTTTATTGTCGCTTAGACTGTTTACCGATTTTGCCTCATCGCAATTAGCACATTTCAGTATTGTTAATTGGGAAAAACGATACAATTTTGACGAAATATATGGAGCAGCCAGGTGTTGGGCGCTATGATAAGCAGTGCTCCGCCATTCGATTGAGTCACCTTCCAGGGAGAAGCCTTTACGATTATGGCGATAATAATGGCCACCTGTTCGTTCTTTTCCATTTTGTTTGTTTTGTGAACCACAGCCCGACGACACTCGCCACAACCCAAAGCTATTGCAGCTGATCAATCTGACGGCGGTGGCCATCAAGCGGCTTATCAAAATGGCCAAGAAGATTACAGCATTCCGTGACATGTGCCAGGAGGACCAGGTGGCCCTACTCAAAGGTGGCTGCACAGAAATGATGATAATGCGCTCCGTAATGATTTACGACGACGATCGCGCCGCCTGGAAGGTACGTAGCCTTAATTAAATAACTAAATATTCTATTAGACTGATATGATATGATACAATGTGCTCCTACAGGTACCCCATACCAAAGAGAACATGGGCAACATACGCACTGA
 
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