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CRIMIC CR01879

Gene Ank

CG1651

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	4:129849..115074	102A3	FBti0213013	91341

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAGAAACACCACTTCACTTGGCAGCT	AATCTTCTAGCACAAGCACGCACACG

Images

Construct Design Sequences

sgRNA sequence	ACAGCGAAACGAAATACTACGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGAGCGGGGAGAGATCAATT	CTGTTGCTACCGCTCTCACT	TAGCTTTTCGGCGATCACCA	TGGATGCTATTGCCCGTGAA

 

Amplicon 5'

Amplicon 3'

CGAGCGGGGAGAGATCAATTTCGGACGTCACCAAAAAAGGGTCTGAATAGTGCAAAACCAATGTATGGCCGTTATGCGTCTTATTATTCTAATGTCTGCTATTAATTAGTGCTTTAAGTACTAAATTTTTATCAGTGCCGTGTTAGATAAGGAAAATTTACCTTTCTTATTTAGTACTGAAACATCAGTAAGATCGTGGTCGGTGTTTGTAGTATCGAAGTTCTTCTGATCCTGGCCGTAGTTGGCTTTTAAATCATCAGTTGCCATGTATTTGTAATGGTTATGATCCAAAAGATCATCACAAGATTCGTCATCGGAATCGGAAAGTAAGGTTTCTTGCATAAGCTCTGGAGTCATTACCTTGAGTTTTTCTTCAATCGCACCAATATTTGAATTAATAACGGATGTTGAAGTGACAATTTTGAGGGATTCCATTACAGTTACGTATCCAAGGTTACTTGCAATGTGGAGAGCTGTGTTCCCGTCCTAAATTAAGGATTTGTATTGAATTTGTAATGAAAAAGCACGATAATATACAATATATTTATATGGATATGACACAATCTATATAAACAAAACAAAGTTGTTGACTAATATAAGGACTCTAACGACTCGACTTCCTGGCGTCCGATTTGTATGGCTTTTTTTCTTTTAGATTCGTAAGGAAAATTTTTTCTCTTTTCTGGATTCGTGTTATATTTGTGTTTATTATTGGTTGTTTCGCTGACTCGTTCATTGAATCGTTCACCTTTAAAATGCTAAGTAAACAATCATTAATAATCATCTCACGTTTTCTTTGCAAAAAAGCATGCAAGGCGATTTCATTCTGAAGTGAAACAAGTGAAACAAACAAACGACGGTCAGAGCATAGCACTTTCTTTTTCGTTCTGCAGCTATGGCTCTAAAGCTTTCTTCACAGTGAGAGCGGTAGCAACAG

TAGCTTTTCGGCGATCACCACAAAACTATTAATTATGACCATTATTTTTGACCATTTTTTAGAACAGGCTTGGAAATGTTAAAAAAAAAGTTCCAAATATTACAATAGCTTTAATAATAATTCTATATGACTTTCTTAGGATGAAATAAAATAAAATAAAATACTTACTTTTGTTAAAGCATTTGGATTCGCTTTATGGCGTAATAAAAGATTAATAATCATAATGTGACCTTGCTGAGCGGCTTGATGAAGCGGGGTATACCCGATATTAGAAGACATCTCAATGTCAGCGTCGTTCTCAATAAAAAACTTGACAAGATCGAGGTGACCATAATGGGCAGCCATATGAAGGGGAGTATAACCATTCCTAGTGCGCTCTGAAATGTTAGCACCGTGCTCCAGTAAAATCTGTGATACAAGAACATGGCCCTCCTGGGCAGCCACATGTAATGGTGTTAGGCCATTTTTTGCAGCGGCGCTAATAACTCCATACTCTAGAAGTAGTTGGACCATATCAACGTTTCCCCCTTGAGCCGCCAAATGTAACGGAGAGAAGCCACTCTTGCTAATAATATTAACGTCTGCACCGTGTTGCAACAGCTGCATCGCAATTTCCAGATAATTTTTTTTGCAAGCTATGTGAATAGCACATTGACCATTTCGGGCACATAAATTTGGCGATGACCCATTTTTTAAAAGGAGTTCTACTATTGAGGGATTATTGTAGTGCGTGGCCACGTGTAATGGGGTCACATCATTTTTCCCCTGAAAATCAATAGAAGCTCCATTTTGTAGTAATATTTGAACCACATTCTGCTTTCCGTATTTGCAAGCTAAATGCAGTGGAGTAAACCCTTTTTTGGTAACAGCATTATTTTCCGCACCATTTTCCAGTAACACTTGGACTATATTCTCTTGTCCTTCTTTTGCAGCAATATGCAGCGCCGAATATTTGTCATTAGATTGAGCATTTATCTCTGCTCCATGCTGCAGTAATAACATAATTATATTAATATTTCCTAATCGAGAAGCTACATGAAGTGGCGTTTGGCCTTCACGGGCAATAGCATCCA

 

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