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CRIMIC CR01881

Gene SPR

CG16752

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:5446656..5494012	4F11-5A1	FBti0213441	91423

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAAGACGCGACGCTCATTGACCTAAA	ATGATGGTCTCGGTGGCTGAAACAGT

Images

Construct Design Sequences

sgRNA sequence	CATGGGCAGATAAACTCGCCTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCTTTCCCAGCTTTTCCTGC	ACAATGAAACGGTTACCCCCT	GCCCATGTCGCTGTAATTGC	TGCTTACCTTTGAACGGCGA

 

Amplicon 5'

Amplicon 3'

GCTTTCCCAGCTTTTCCTGCCCTTTGCACGTTTGCCTTGATTAATCAACATTTTCAATTTATGGCACTCGAAGGGCGCGGAATGGGGATTCTGGGGCTTGGCTTGGGGGGTTGGCAAAGCAAAACTGTTTACTCACTTCAATGGAGTAGTGTCAAGCTGATAAAATGCCCACCCCCATTTGGTCATAAGCATAAGCCCTCCTCAGACTCTGGCATAGCCAAAGGCATAGTGATTTACAGAAGAAGGCTATGGAAAAGCTCGGGTGTTGAGCGGAATTAATCCATTTCAGTGTTGGATTTTCCAAAAGAAGATAAGCCAAAAACATTTACCTCAAAAAGGTGATAGACATTTGAATAATTAATTCTTTTTAAATGGAATTAAAAAAGAATCAGCAGTGCTAGAAACTTCAAGTCAATGAGAAATATAAAAGTACTTATGGTTTTTTTTTTTGGATTATGACCATTTTGCAAGCGCTCTTGCCCCACTGTACTGGCTCATTGTAGCTACGTGCCGTTAGTGGACTCTCCGGGGATTTGTACCAGCTAAATTCCATGCTGGGCTGGATGTCTAGATGGATTCCAAGGGACGGGGGCCAAGAATTGAGATGGCTGCTTGGCCTGGTTGTGGCGGTGGTGGGGTGGTAGCAAGGTCCTTTGACTATCTCTGCGTATGACTGCCATAAAATTCAACTCACATTCGCTGGAGCGGAGCACTCCTCGTTTTGGTAATAACCACCAGGCGGCCATTGCAGTCTCAAAGTTGAAGATTCCTTTTCATTTCCACCAAAAATTTATATGCTCGTCGGCAGCACGTCCTGGCCCGTCTTCATTCCGGAGATCTCCATGGCGAGTGGTGCGCATAATGACCATGTTAATGTGAAAAGGGGGTAACCGTTTCATTGT

GCCCATGTCGCTGTAATTGCACACACACACACACACGCACACGCACACATAAACCGGATTAGGCCACAGATTGTGCATTATATGGCCAAGAGTCCGATGTCAGGCCCACAGTATCTCGCCCATGGAGCGCCTGTCTGCCGCAGAGCCGCACTCATAATGGTCCATAAATGGAGCAGACACCTGTCCAGGTGGCCATTTGCCAGCTACCATTTGCCAGTTACCATTTACCAGTTACCATTTGCCAGTTGCCAGGTACCAGTTACCTGGATTTCAGACGCCACGCACGCCATTCCATTCTATTCCATCTTACTGCCGGCTAATGCAGTTGAGTACCACAAATCCGATTTGGCAGACAGGCTAAGGAATGAGTTTCTGTGCCCTTGGGCCAAATGCTGATTTACTTACCTATCGCAGACATATATCTTGCTCAATTCTCATGGGTATAATGGGTTCGTATGATGGACATTAAAAGATTTGTTACTTTTTAATGGCAAAATATAAAAGGGATTTTGAGCAGTATTCATTTGAAAGGATTGCATGAAATTTAATGTAATAGGTAAATATTGTAAGATCAAATCACATATAAATCAAAGAACTATGATTTAAGTCAAATAGCTGAGAAGCATGCCCGAAAGTTGGCTAGTAAACACACACAAACGGCGCTAGTTTCATCTGCTACCAGCAAACCCTCGCTTTCAGTCATCTGTAACTATATATAATATATATATTTACCTGTACTTTTGCTTACAGATAATGCCAGCCATGTGCCACACCATCTCCGTTTGGCTAACTCTGGCCCTCGCCGTTCAAAGGTAAGCA

 

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