• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR01883

Gene Nrg

CG1634

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	X:8517373..8555170	7E10-7F4	FBti0213442	91424

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCTTCTTCTCGTTTCTGGCAGTCTGG	CTGAGCGATTGCGGATGCATCTACAA

Images

Construct Design Sequences

sgRNA sequence	GGGTGAAAGGTTATAGTGAGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTATGCATGTGTGGGAGCCA	CCCCTCTCCCACTTTTCGC	GCGGTGGGAGTCAACTGATG	TTCTTGTGCCCCCATCTTGG

 

Amplicon 5'

Amplicon 3'

TTATGCATGTGTGGGAGCCATGGCAGTCATATTAACTTTAAGGCACACAAATACTTTGCTTTTCTTTTCTTTTGATGCTTTGAAAAATGCCACGAAACTGAAGCAGAGCTTTTCGATTGTCATGGAAAATGCTTGTTTATATAATAAAAAAAACTCCATACTAATCTATTTAGTTCATTCCCTACTCACAAAGCTTCAGCTTAAAGCAGCCAATTAGAATATTATCGCTTATCCTTTCGTACCCAGAATATATTCCACATACGCAGTGTATGCCAGCTGCTGGAAGATAAATACGAGTAGAGATAAAAATATGGAGAAATGAGTGGAGTTTGAAGGCTCATTGATTTTTCACACCACGCTGCACAAAGTCCTTCAAAAGCTGTCGCTACTAGTTCATGTCCTTGAATGTTATTTTCCTTCATTCCTCCACTTGGCCCACCCTCTCTCATTTCCCGACTTCCCCTCGTTAATTTTGTTATTTTAGCTTCATGTTTCCCTAATGATTTTGTCTCGTCAGCTTGGCGCCAGCTTGGCCGCGACCACCCCCAAAAAAATTCAAAATCAAGGGAAAAAAAAAAGGGAAAACAAAATGTTGGGAAAAACTGCAACTGAACAATTCGGTTCTTAAGTAGTCCGATTAAAATCCGGAAATTGCAACCCCAAAGTGATGGAGTCATCAAATGACTTGGTGAAAGATTGAGTACGGTGAAACCTAGCTAAGAGGAACGCAAACTTTGCTGGGCATACAATATTATTTAAATATACGCACCGATGTTGTTTAAAGCATGATGCATTTCTTAAAAGCATGGTCACACTGCTCGTGTTCTGAAAAGCAACTCTGTCCTTGAATGGCTTAGCATAGCCAAAGTCGACTGTACTTCCTTTTTGGTCTTTCTCGTCTCCCTTACAGAGCATGATTCATGCGTATCAATCAAACTTTTTGGTCAAAAGGGCGAAAAGTGGGAGAGGGG

GCGGTGGGAGTCAACTGATGACGACACGTCCCCATTGTTGTTGTCTCTTGCCATTTTTGTCATTTATTCGGCTTCTTTGGCTCATTCTTCCGACTTATTTTGGCTATTGCGCATATGAAGAATTAACGAACAATTGTGGAAAGGGAGTGAAAATCGAAGGGAATCTTAGCATATTTAATGCAGCCAAATTGAAGAAAATCGATGAACTGCGACAGCGGCAGAAATAAGACATAACGAGTAATAAGAGTAGGGCTTCCTATATCATTTGTGTTTTATAGCTTTGGAGGGTGGAAAGTAAATGGAGATATTACTTATTTACTTACCGAACCGAAAGTGACTCATTGTTCGAGGGTAATAAATGATGTACTTAGAAGATTGATTATTAAATCCTACGAAAGCTCTTAATTGTATTACAAAAGTGAAGACAAGAAACCATGAAGAACGTTTGAGTTGCAGCAACTTCAACTTCATGAAAGTACTTATGCACTTAAATAACCCAAAAGATCTCGGGATCTGCTCCATTTCTCCATTTATTATGCAGTTGAAATTCTCCGCACGATGAAATAAATCATAAAGGTCTGCACTCAGATCTCTGGCTCAAGATTAAAGTGAGAAAAAAACCAACCTGTGCTCATAATCCGAAACCCGAAAAACTGAAAAAAAAAAAATTTGAGAACTTTGTGTTGTAGCTTTTCCCAAAGTTTTCTCACGTTGCGTAGTGCATGCATTCACGATCGTGATTCCTCGATTTCCCAACCATACTTCTCGGCTAACTTTCTTCTTTCGCCGACGACTTCGTCTAACGGTGGCTCGAGCACTCATTTAACCCTTTTACGGTTTTATATGTTACAGTGGAGCCCAAGATGGGGGCACAAGAA

 

---

Search Again