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CRIMIC CR01885

Gene syd

CG8110

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:7916650..7936120	66A20	FBti0213443	91425

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCACCTCACGCAATATTCTGCAGAGA	CGAACGTCTCACCTGTGACAATCTCA

Images

Construct Design Sequences

sgRNA sequence	AAAGTGCAATTGATTAGTAGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCACGCTCTAACGTTCCACT	TGTTGTCCCTTTGATGCCCC	TGGGACAGAAACTGTAGAAACCT	CACCCCAATTCCCCGATTGA

 

Amplicon 5'

Amplicon 3'

CCACGCTCTAACGTTCCACTGAGATTGTCCAGCTCGTTCTGCAGCGAGGGCGGACCAGAGTCATCCGACTGGCTGCCCACACTGCAAATGGTCTCCGTGTCCAGCATCACCGAATTCTCCAGCGAGGCGAAGCCATAGGAAACGGGTCCCGAGCTGCGGGCCACAGGATTCAGTCTCGCTCGGCTCACATCCATGCGTTCGGAGGCTGTGCTCATTTGTGAGTGCGTTGAGCCCATTAGCATCTTGGTGCGCTCCATGTAGTCCACATGGTTCTTGAACAGCTCTGTGTACCGCTCGTGCAGTTTGTTATACTCCTGAGTTGGTTAGATAAACAAGCAAAACATTTACTGTCTGTACTATGGCTTTTGAATTATTGGGGTTTACCTTCTTGAGATCCGCCTCCCGCTCTTCCAAACGACTGGCGTGCTCCAGACTGTTCTTGTGCTTCAGTTCCAGCATTCGCACAATGCTCTCCACCGATTCCAGGCGCGTGGCCAGCTCCTTGTTCTCCTGCTCGGCCAGATCCTCGGCTTCCAATAGCTGGTGGCATATAATTTATTGAATTTAATCCAATGACTTGATTAGGCTCCAACCAATTTGCTCACCTTCTGTTCGGACTGCTTGCGGGCGCTCTTTTCCCGCTCATATTGTGTCACCAGCTGCTCGTTGTCCTCACGCAGCAGCTCCACCTCCACGTCCTGCTCCTGATTGATGCGATAGGAGGCATCTAGGCACTCCAGCACGTTCACGAGCAGCGGCATCAGGTTCTTCACCACATCCTCGTCATAGCGATTGATCATCCGCTCAAATTCCTGATAAATACTGCCCGCCAGTTGTTGGACCTGAAAGAGGAATTGCATCAGTTGATTTGTGCTTCGTTTTTGGGGCATCAAAGGGACAACA

TGGGACAGAAACTGTAGAAACCTTTACATAAATATAGGTTTAATTTTTCTAGGGTATTTCACTTTCAAAGAGGGTTTTATTTACTCAAGAACCAACATATTCACACAAACATAGTATCAAGTAGAGATAGTAAGAAACATTAAGTCGTTTAACATAATTGCGATATGTGGCCGCAGTTTATGTGAATGAAAGACGCATGCATTTTGTTATAAAACAAAGTAAAGAAACTCAATTATTTCCGGTAAATTAAAGAACCGAGAAAGGTTAAGGTTGACACCTTCTCAGCTTTTAATTGTTGGTTAAAAACAAATGCGAAGCGTGCAAAATGGTACTCAACTAAATGGTTTAAACAATTATATATCGTGCATTTACGTATTAAGATGTGTGCAAGAAAAAAAAAGATTATAGGAGAGAAAATAAATAGACCACAAACGAAAAATTTTGATAACTCACGATGCTGACAACCTGTTCATTCTGAAAGAGAAAGAAAAGAACAAAAACATGGTGTAAAGGACACATAAAAAACAAAATTTTCAACAAATTGATTAAATGAAAAATGTGCAAATAAAACCCGTCTTATGAATAAGTACATGATGAATGAGTGAGGTTGGTGCAAAATTATTTACGATAAATGTGTTAAATAAGATAATAAAATAAAAAGATATACCATGCGTTTTCACGTATTTAAATGATTTTAATTGGAGTTTAATTTAGTTTTTAAGTTTTACACGGCATTTCTTGGCATTTACATTGCAAGAGACGGTAATTCTCTAATAAAAACCCGTCAACCGCCCAAGGTTGGGCCAACCTATAAGGCCAACTAGCTAAAGTATTCAAACATTTTTGGTCAGACTTGTTTCACAAATGGTTTCTGCGCTCGTTGGGCTAAAAAACAAGTTTTTATCGGCGTGTCAGCAATGCTTCCCGCTTCAAAGTGCCTGTTTGAACTCATTACTCAATTAACAGGTAGCTTATCAGCCGCCCATGTGCGCCAAGAACTATGAATTGGGGAACCTGATGAGCTCATTTCGTGGTGAGGATAGCGAAATAACACTTATATGAGTACGAAATTGAGAGGGAAGGCCCCCTGATTTGATTTGCATAGACCGATTAAAGCTAGCGAAGCTGATAAAGTGCAAAATCAATCGGGGAATTGGGGTG

 

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