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CRIMIC CR01886

Gene CG13728

CG13728

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:17382728..17380026	74A6	FBti0213014	91342

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CATGCGGTCCTGTTATGTTATGTGCG	AGGGCATAGTCCTGCTGAAATGGATG

Images

Construct Design Sequences

sgRNA sequence	AATCTAGACATGAGGAAAGGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GTTTTCCGTTTCCCACTGGC	GGGGGCCGTAATTGTACATCT	TCCTCATGTCTAGATTTTCGCG	TCTCGTGGGTTCCTCCTCTT

 

Amplicon 5'

Amplicon 3'

GTTTTCCGTTTCCCACTGGCTGCATGGCTAACAGATTTACATCCTGCGGTGAACGGGGCTTGTAAGGATACAGTTGATCGATGGCCACCCTTTGGTGGGGCATCACACCGGGATTATACCATGGCATTGGCATAGACATCATGGGGAACATGGGCATCGGTATGGTGAACTGCATCTGTTTCTTGTTGCGCTTGCCGCGAAAGAAGTTCCCTATTCCACTGGACTTGAAATTCTTGAACGTACCGGCGATGTGTAGACCCGTGGGCATCATCACCATTGGTGGCAGCTTACTGGGCGCTAAGCCTCCGATGTGCAGATGCTGCAGGATATCCTGGATGTTGTTAAGCTCGGGTCGACCCTCACCCTTAGTGAACTGTTCGGGCATCGGACCATCGTAGGGGAAGTGGAACGGCTTTGGCTTGAACTCATCCTGACCTCGAAGTGCTCGGGTTCCCGGCGTTTCGGAGTACTGCCTTCGTAGCAAACCCTTTGTGGGCGCCGGTGGCGGAGAGGGTGAGCCCAGGGACAATTGCATATCCTGGGTGGTGAGGCGCGGGGGGCGTGGCATCTGATCTCGCAGCAACCCCCTCAGTGGCGTCATGGTCGCCGATCGCTTGGGCAGGCGCTCGTAAACATGGGCGTCGTCCGTTTCGTGCGAAGATGTTGCGTGTTGCTGCCGTTGCGCTTTCGTGCGCCACGTCCCGTAGATGGGTGCAATGTTGCTATCGGCACGCTCCAATAAGTAGGCGGTTTCCCCAGCAGCTCCCTGCGTCGCCTGCATCTGTTGCAGATGCAGCTGCAGTTGCTGTTGCTGCTGGCGCAGGTACTGTTGCTGCTGCTCGTGCTGCAGTTGCTGCAGCTTGGCCCGCATCTTCTCGATGTCGCTGTCGCTGTCCGAGGGCTCGATCTCCGTGGTGGCCTGGGATGGCTCGTCTGCTGAGGACTCAATGTCGTTCAGAGATTGCTGCTGCTGGTGCTGCTGATAAAGAGTGGCTGCGGAGGCGACCTTCACTACCATGAGAGACATCTGCGGGTACACACAAAATGAAATCAAAGATAAGAGATAATTGGGAGATGTACAATTACGGCCCCC

TCCTCATGTCTAGATTTTCGCGTAATTGTTACTCAAAATATGTAAACAACAAACAAAAATTGTCCAATTACACTGTACTGCGTAATAAAAAAAAAACCCTTTTGTATAAGAGGTTCGAATCCTCAACTCACTATTGTGTACACCAGGATCAGGCCCATAATCCACCGCCTGCCTTTAGTCCAGCAAGGATCCAGTCGCGTTCGAGTGTCCCGTTGTCCTTGACCGACTGCCGACTGCATGAACATCTTTCCGGGATCTGCTGCTCGAACCACTCGGCTTGCCTATCGCTTGATGAATGTGGGCCTGAGCTTCTGCCTCATCGCGGATCGCAACGATGACTGATTGCAACTGGGAGCTGGCGGCGCCTAAAAAGCGTCGGTTATATCGGCCCAAGTGCAACAGCTTAATTAGAAAGTTTAATAGAGTTTGGCTGCGAAAGCAAAACAGCAAGCTACAGACAGATAAATAAATTAATTGCTCAGGCGAATTCGTGCTCCCAGGCAAGTGGAAAAGAAAGAGATATTAAAAAACGCATTGACCATGAAAATCTGAAGATAGCTGCGAAGTAACTAGAAATATCTTCGGGGCGTGATGAAACCGAAGTGGTTATGCACTCACGAAGGAGCTGACTAGCCATAGCTCTAATTTTGTATATAAGTGAAATACTTTTTAAAAAGGGAAATGATAACATTTAGTGATAATCAATAATTATATTAAAAAAATCGATTCAATATTTTAAAAGTCATAAGTTCAAATCACATTTTAACTATATCTCAAATTTGACATTTAATTATAACCTAAGGTTAAATTATTTCATATTTCATGAAAATATACATCGCTCATAGTAAACATACATGTATTTAAATACCTACTCATACGAACTTTTCAAACCTATTAACTAAGCTAACGTGTTTCACATCCATAACTTAAATTAGTAGAGACCAGAAGTGGAGGTCAAGGTTAACTGCCTAACCTGCACTTTGGGCGCTTTATGACTTGCAAGCAACGATCTCCTTTGCATAGTCGTTAAAACCGACTTTTAAGATAACTCCCAAAAGAGGAGGAACCCACGAGA

 

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