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CRIMIC CR01888

Gene CAH1

CG7820

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:13803626..13809994	34D1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GATATCAGTCCGATCGAAACCAGG	GAACATGTCGCCAGAGTTGACAATCG

Images

Construct Design Sequences

sgRNA sequence	ACGAAAACCCGTAGCTGTCTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGCGAAACGATTTCTCACGC	CTGGGAGAACTTGTTCCGG	ACGGGTTTTCGTTTTTCCGC	AGATCGCCGAGCTTAAGGTG

 

Amplicon 5'

Amplicon 3'

TGCGAAACGATTTCTCACGCAAGGAAAATCATCAGATTGATATTATTTTTGGCCAGCTATATAGATACTGTATCACCTGACTTGCCAGTCCAGCTATTTTATATAGCAAATAAAATTTGATAATTTATGCATTTGTGCATATATTGAAAAATATCTGGACATGCAAGAAAAAACAGCTCAATTAATTTTCAGACGTGCCTAAACATTTCATTAGGCCACAATGCATTTCAGTTAGCTAAACTTTTACGGCCATTCGAGTATTTCATTTGCGCTTTATTGTCACCATTTCCCACCACATGCAAACAAATAATTATTGGCGAAAATTTTAGCAGATGATTGAAGGGTTGCATGGTTTGGATTGGTGGTCGATAGCCAAATGTCGTGGCAGTCCAAGGGTAGAGACCATTTCTGGGGCTTCTTCAAGCCAAGCTGGGTGATAAATATAGCAGGGGTAGTTGCCTTTCAGCCCGGTCAATTGAGTGGCCATAAAAAACAGTATAACCCAGAGACAAGTGGCCCCCCTCCCCACGAACAGCAGTTATACAATATATCATCCGGTACTGACTCAGAACTCGGAACTTGGAATTCCGGGGTGCGGCTGGGGGCACTTTCCCCAAACGAGTTTTTTGTCCGTACTTCCTTGCGATCGCCGCCAGGAAAGTGGGGAAAAAACACGCGAGAAACTCATGCCTCCATCCAATTGGGATGATATTATCATGCAAGGCTCGCTTAGAAGCGCTCGGCTCGTAAATTCGTAAATGCTAATTGCCACATTTTATTTTGTCGTGTTTTTAAGCGTTTTTCTTCTTTTTTTTTGCACTGTCGTAACGAGAATTTCTTAATTTGTCTCTCCGGTTTTACATGCGCTTTTTATATTTTTAATTTTTTTTTTTATATTTCAAATTGCTGCTTTGTGTATTTAAATGTTCGGAGAAATCGAAATCACTATACTCAGGGGCAATTTTCGATGGATTTCGTAAGAACGATCATTATACATTATAGATTCTATATAGAGATCAAGATCTTAACGTTTCCGGAACAAGTTCTCCCAG

ACGGGTTTTCGTTTTTCCGCCGGCGAATATACGCTATAAATGGATGTGGGTAATTCATTTGCAGTAATTCAATTAAAAAATCATTCGGATTTTATGGCAGCATTAATGGTCACAATTGTTTCAAAGCTTCGCTTTTGTTCCATGTCATCCGAATTGAAACGAAATTAAGTGCAAAACGATGATGAATTGTTTAATATACATATAACTTTGCAGTTAGTAAACGCGTATAACAAAGGCTCGTTTGATCAGCTTTAAGGGACCCATAATGTCAACCGGTTATATCCGATCGAGTTTTGTTTGAACAAGTGCGGCATTTCCTTGTTTTCGTTTACTTTCTTCTACTTTTTTTTTATTAAAGTCTGCCAATTGTTATCACAGGCACTGTGAAATGCCGCCAGGTGATCTTAAAGTATTTACAGCCAAAGTCGGCAGCAAACTCGCTGGGTAAATAAAGCCAGTAAGTAAGCCTATACATTTAAATGAGTCGCACTATATTTATTTCTACATATACATATATACAGTACCGACTTCGGAGATATCTTAATAACTCGATTGTTGATTATAAAAAGGTAATGTGCTAAATGCGGCAGTAAATTCGCACTTGGCTGCTAACTCACAAAAGGATCGTGCAACTCCAATTGCCTGCTTCTTTGTATGCTGCCCCACACGGCGTATACGTGACATTCATACGCCATGTGCTGCGTCAGTCACATGCACACACTTTTTGCACCCGAATGGTGAATAAGGCTCTTGATTTCAACTTTGATAAATGATAAATGCATTTCACCTGACTTTTGTTATTTACTATCAGCATTATGAAGGACTTTGAGAATCGGCGAAATAAATATAGGAAGCTACAAAGCTGTTGACACCTTAAGCTCGGCGATCT

 

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