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CRIMIC CR01889

Gene miple2

CG18321

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3L:275018..271200	61B3	FBti0213015	91343

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGAGCTGGTCATTGAAGTCAGAGTAG	TTCTGCTCCTCCTCCTCCTCAAGTTT

Images

Construct Design Sequences

sgRNA sequence	ATTCCCATAAAAGAGCAAGGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATGCGCTCTTCCCATCCAAA	GCCCGATTCTACTACGTTCCC	TCATTGCCGGCTTAATGGGT	CTGAGGTTCACACGGAGTCC

 

Amplicon 5'

Amplicon 3'

ATGCGCTCTTCCCATCCAAAGGGCAGCTTGTTTGGCCTTTCGGAAGTACGTTCTTCAGTTAGGTTCGATAGCCCACTGTCCCCATCACCATAGGAGGCGCTTTTCACGTTAACACTCGAATATACGCTTTTAGAGCTCGCGTCAAAGCAACCCCAAGAGTGGTCACAAATCTGATCTCCATTTTTGCCATCTTCTTTTTTAAATATATCAAACTTGCATAGCGCCGGATTCTTGGAGTTCATTTTTGCACAAACTAGACGGTGTAGGTATTTATGTGACAAGTGTAGGTACTGAATAGGTTACTGCTTTGAACCAAAGTCTTAAAGAAAAATAAAGCAATGCATTCTTGTTTGTTTTACAGCTCGGCTTTAAAGCATATATTTTTAATTATATTTTATTTTCATGACTTTATTGTTTTTAGGTTTCCGAAATAGTTATGAATCTTATACTGGCTTTAACCACGGCTCTGCACCTAGTTTTGGTGGTATTGGCGCACAATCCGCTATCAACTCGTGGCGAAAGCCTTTCTGATGGCATACGTCTGGTCATGGAAAAGAGGTCGGGCGATGTGGTACACATTCGTCCAGCTCGGGGCACACTGGAGGCCGAAACAGTCGCATCCACAACAAATAGTGACACCCACCGCACCAGGAACAATCGTAAGCCGAACAAGCCTCAAGAGCACTCTCAGCAGGTTGGGGCCCACAAGTCGGGATCTAGAAAACTAGTGGTTGCGGAAAATGGCGGGGCACTGTCATCACAACTACCACAACCAAATCACAAGCAAGGCCACAAGAACACTGATAAGCAACAACGAGGAGGGGGATCCAAGCAGCAGCGAGGACATAGCCAACATCATGGAGGAAATAGGAAAGGGCCTAAGGCAGCGACTCCAGAGAATGGTAAGCCTTAGGACTGCCACGTCAGAATTCAAATAGTTTTTAAATGCAAAGTCTTATATTTGCTTGTTAACAGGATCGACTTGCCGATACGCAAAGAGCGCTTGGTCCAATTGTGATCACAAGACCAACATGCGTTCACGAGTCCTGTCCCTAAGGAAAGGAGAGCAAAACTGCTTGCCCACGCGGACCATACAAAAGAAGTGCAAGAAAGGTGCGCGCGGTAACTTCTATATGTATCATAATCCTACACAGCGCCTCCCCAAAAGGGGAACGTAGTAGAATCGGGC

TCATTGCCGGCTTAATGGGTCCAAAGGTCACAAAATGCAAAAATCACTCTTGTTGACCCATATACAAGAATGCTTCCTTCTGGGGAGGATTGTGCTCTCTTCGACCCACTTCGAGGTTCTCCCCGCCACAGCTTCGTTTCGTTTCCTAGGAAATACTGCAGTTCCTGTTCCTAACAATGCAGCGAGTGCATCCTTCATAATAGCAAATACTTTCCAAAATTAATTTGAAATTTTGCGTACTTCCTTCGGTAGGCTGCCGCTATGAAAAGGGTGAATGGTCTCAATGCGTCGGAGGACAAATAACCAGAGAGGATAAGTTGGAACCAGAGGCAACTGGCGGTAGTGATCAAAACTGCAATCCTGTGCGTACCGTCAGCAAGAAGTGTAAAGCAAATGGCAATTCCAGTGGAGGAAAACAACACGGCCAGAGTCGTCGAACAAAAGAGCAAAAGCAAAAGGATAAGGGTAAAAGTGGTGAATTTGACCCCTTTGTGAAAAGTTTGATCCAAATTCTCTGTTTACAGGAGCCAGTCACATTTCGTCTTGATAATGAAAATTATTTATTTTATACGTATACGGAAGCAACATTTTTAATATTAAATAAGCGATTTTTAAATGCTAGCCCGTACGGACTACTTGAACAACAATAAAGAATTGGCCTTCTTAGGGCTAAATGAAAGCACTCTTTTAGAAATTCTTGAAACAAGGCTTTCACAAACCCCAATACCGCGAAAACCTGCACAAAATGAGACCTGTTATTGAGTAATCAAAGAGCATTGATAGAACTGTTAGTTGGCTCTGAGTTTTTATTTAATATTAGACGCGACAGTAAAATAACCGTCTGCGACAAAATTTAATTAACATTACACAAAAGAAAACGTTACCTCTAACTCGGCTTGGTAATATAAAGATGTTAAATTTAATAGTGTTTCTTTACTTAACTGCAAGCCCCCTTTAAATTGATCGATCTTTCGAGGCAAATCTTCAAGTTACCAATGTCTTTAATAGCCATTGAAAGGGTGTTATGTGGCTGGCCCAAGATTTGCCGCAAACTTATTGTAACGTCGCGGTCGCTGACCTCCGGCTTGCGGCGGGCACTGGTCAAGCAGCCCCGTAAAGGCGGAGATGTTGGAAAACCCGGAATGGAGCTTGGGCGCTGTTCGTGCTTTGGACTCCGTGTGAACCTCAG

 

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