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CRIMIC CR01891

Gene CG32036

CG32036

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:9489233..9488050	67B10	FBti0213016	91344

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGCTCACCGTTTAGTGCACATCCATT	TCGATCGAGGTAAGTATGTCCGAGTG

Images

Construct Design Sequences

sgRNA sequence	GCTGTGTCACGCGGGGATTTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CACCTGGGGATTGGAAACGT	GGGGGTACTAAGACCAAACCG	CGCGTGACACAGCCTCATTA	TTGGCCAGGTGAAATGGGAA

 

Amplicon 5'

Amplicon 3'

CACCTGGGGATTGGAAACGTTTAGTTTTAGTCATGGATCAGGGACTCCTGGGACGTCATTTATTAGCTCACCTCTCGTTTTTTTGCCACCTCGTCGTAGGGGAGCAACTCCAAAATGTCCTGAATTAGTGTTTGTGGCAAGTCTAACAGATTCATATTTCTCGTTTATACTTATTACTAGTTTCGAGCGGAGCGATTATAGTCGAGAATATTGCTATTGTTTTTAATAAGACTGTGATGTCAGCTCATGGAAATGCTGAAAACGCCTTGTTTAGCCACAAAATACCACAGACTAAACCATGAAAACATACCAAAATAAACATACCAAATAAATAAATATCTTACAGTTATTTTACATGTGCGTCAAATTAGCGTTGGTGAGTTTTTTATAGGTGAATTCCAAAAAATAAAAGTGCGTAAAAGAAAAGAAAAATCTAGCTACGTATGTACGATTTAAGTTTATATCAAATATGCATATAAAATATCAAATATGCAATTGAAAAAGGGGCTACCCACTCTTTGTTATAATGTTATGTTTTTTATTTACAAGATTGGGAACAACAACAACACAATGAGAACGGAAATGGATTTAAGCTTGTATCAGAAATTTGCTAGCATCATGAATATCATTTGCATAAGGTTCCAGCTCAGGTTTCTTTTTGGGAATGTAGGGATTGTGCTCGGGATCGGCATTCAAGCTGTAAGAAATACCAATAGTTTAAACAAAATAATATATACAAATTAACCATACAACTTACTGGTAAAAGTGGGTAGCCTCCTCACACTTGACATTGTACCACCAATCGCAGGCGAATTCCTTCTGGTTGAAGATGGTGCCGTTGGTGCATAGGAACTTGGCGCCCTGGTCACCTTCGCGTCCATCGTGGCACACGTGGTACGCCTGGCAGCCCGTCTCCACGTTGGCGTACATTCCCGGAGTGGCCGGCACATTGTGGCAACTGAAGTGGGTGTGTGGCACCTCGTGGTAAATCGGATAATCAACGCCCGGAACTCCAGGTATCTTGTTCAAGTCTTGCTTCTTCTCGTGATGCTGCGGGTGTTCCGGTTGCGGTGGATTGAATAGATATCCGAATTTCTGTAACCATATCAAATGTTTCTATTGCATAAGACATGCCACTGCTCGTGCCCCAATCCGGTTTGGTCTTAGTACCCCC

CGCGTGACACAGCCTCATTATAACAATACTCGTGGATAGATATACTTTGATAGAAATATTCAAGAGACTGTAATCATATTTACTTTCAGACATCAAACTCTTCTTATGCTCAAAAGTAAATAAAATATTTGTTAAATAGTATATTTTCACATCATAAATCATAAATTTTTTATCAGTGTACACATTGAGTACATTGCATGCTACAATTTTTGGGTCAAAAGTGAACGTGGCTAAGACACCCAGAGCTTTGTCAATTGGGGTGGCACACACTCCTCCCAATCGACTCCCTAGCATAATTATACACTCGTTTTCCGATACCCAATCCTCACCTGAACTTCGATGGCCACCGCGGCTCCGACAACGAACATTAGCCAAAATGAGAAACAGCCCAGCTTTGGCATCATTATGTCGGTTACAAAAGTATCTATGTATGTATCTTTTCCGCCTACTTTGTCGCTAGTTGGTGGTAGCTTGGAGTCTTTGGAGCTGCAGAGACGACCGATCGAGTTGGGTTTGAAAATCACACTGAACTGCAGTTTTCCCAGCGGCAGCCACTTGAGCATGGAGCTTTCAAGGGTACCGATCCGATTTGTGTAACGATTACGATTTTCGCATTGGAAGCTGCCCGCCCAAGCTTTTCCATTCCCCAAAATCGGCAAATTAACTTAGCTCTGCTTCAATTATTGCCACAGTAAACGAACGGCTTGAAGCTTAATGCTCCCCTGCTCCCCACATAATTATGATATAAATAGTGAAGTATACCATTCATGTGGGAAATGCAACATACAACAATGTACGGACACGCGCATGGTTTTATTTTGCAAGATACAAATTGTTTACGCTTAACTAGCTAAAAGCGATGAATTCCCATTTCACCTGGCCAA

 

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