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CRIMIC CR01894

Gene scramb2

CG1893

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3L:3046812..3044113	63A3	FBti0213017	91345

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAGGCTCCTCTTCTTCAGAACATAGG	CCAGTCCGGACCATTGTTTGCTGATT

Images

Construct Design Sequences

sgRNA sequence	CAATGGCGGTCAGGCGATTAGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACAGTCAGGCATGGCTAACC	CCTGACCGCCATTGTAGCTA	GGGCCAGTGATAGCAGTTCT	GAAGGTATCGCTCACGTCGT

 

Amplicon 5'

Amplicon 3'

ACAGTCAGGCATGGCTAACCATGACGTCACTGCCTCGGACTGCAGTCTCTTTGCTTCTGTACGATCACAGCAGCGGTCAGTAAAAGGGCTCATAGATCGGCACAAACACTTGGTGCATTTTAGCCATAAAAAGTATAAATTGATTGATTGATTTTCCTATATCTAGAGGCATCTGTTAAATAACGTATACAATTGAAAATTTAAAACTAGTGTGGGTGCTATTAATTTGAACGCTTCTGTCTGTTCATAATTATTTAGAATATATGTATATAGGCACTTATAAATGTTTAATAATTAAAAAAGCAATTAAGTGGACGCCTGATACTGTTCATTTTGGAATTTTATAAAGCTTCGTCTTTGACTGATGAAGACCATACATATGTACTCCTCCGAAGAATTTAAAGCTAATCCGCTGACCGCTGCTGTGTGTGTATACACTTGTTTGGGCATTGGGAATCGAGATCGAGAGCCAAGAATAACCACTTGTTGGCTGCGTACTGACCTTGAAGTTGGTATCGCTGAAGCACTTGCAGGGACAGACGGGTCCCTCGATCTGCAGGACGGTATCGCCGCATGTGTTCTTGATGTTGAACTTGGGCCGCATGAAGGTGCAGACCTGCTCAACGCTACCGATCACCTGGCCGGGTGGCGCCGACACCTCCACGGCGTTCATGCAGCTGGGAAAGCAGCAGAGGATGTCGCACTTGAAGGGCCGGTACAGGTGGAGCACCTCGTTCTGAAAATTGTCCAGTATCTTCATCTCGAAGGGGCGCGACCGGCCGAGCATGTTGCGGGTGCAGCAGTCGCTCTCCTCGTACGCGAAGTACACATTCTGGCCCAGTGAGTTCTTCACCTTGAAGCGGTTCTTCGTCTCGAAGCCGGTGAGCAGCTCCAGTTTCTCGATCTTCTGCGACACGAGCAACTGGTCCAGAGCGGTCAGGTATTCCAGTCCCTGCGGGCAGTTGGGCATGCCCACGGGTATCGACATCCAGTTTTCTGCAATGGGGCGTTGTTCCGGATTAGCTCCCTATGTATTTATATTTATCTATATGCATAAAGTGCTTTTCCGATATCTATGCATGGGTAAAGGAAATTAGCATAGCTACAATGGCGGTCAGG

GGGCCAGTGATAGCAGTTCTTAACATAAACTAATATTTTAAACAATATGTATTTACGTATTGACTTGGAATCACTTCACTTTTTGGTACATTATAAAATATCTTTTTTACATAGATAACTATGGGTGCTATTGCCATGCCCGCTGCTGTACTAAATGCCAAGAAAGAGACGACGGCATTTCCGCCTTTCGCACACGCCCACTAGCCCACACAGACGCGCGCAACCGACTAGGTCCATCCGAGTGATGGAATTCAACAGTGGACAAATCCATATATGTCCATCGCTACGCACCAGGTCCGTTGGCATTGTTGCCAACATTCGCCGCCTGCGGCTGCAGGACCACTGCGCCATTCTCGTCCTGCGGTGCATTCTGCGGGAGCACCTTGTACTCGCTCATCTGCATCATTGTTATTCCCAGCAATTAAATTCGTATCGGATTCTTTAACAGTTCACAAAACACTAAATGCAGGGTGACCGCGTTGCGAACGCCGAAAAATGCAACGCAAATGCACCGTACGAAATATTCTGTCATATATTTTGGTATTTATTCGAACACAGAAAAATATCAACAAAAAGCTTTCAGCACTCAATGCAGTGACAAGTTAAATTTGTTATGAGCTGACGTTTTTGTGGATTCATTTGTATTATTGCATTGTATTGCAATAGTTTCCTTAAGCTAGCCTCTTTAAGTTCTAAGAATTTTGAGTTTATTTGTGCCAGTTGGTATATTCGCGCCTGCCACCGACGGTAACACTGCGGCTATGATTGGATGATAAGCGATCCATAAAAGCCTGGACACAAAAGACCTGAAACATGCTGCTTGGCAGCCGAATCTCCAGTCGAAATGAGCGAATCTCTTGATAAGACCACCAGCCCGAGCTCGGCCAGTTCCAGGGCCACGCCCCCGGGAACGCAGGATGCGGATGAGGGCCACGACGTGAGCGATACCTTC

 

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