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CRIMIC CR01897

Gene CG7011

CG7011

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3L:15160876..15163628	71B6	FBti0213446	91428

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGCGGAGCACAATGCTACTCAGTAAG	AAGCAAGAAGAAGCGAGGAGGCAAAC

Images

Construct Design Sequences

sgRNA sequence	TAGAGAAAGCCGATAGGGGGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGATCTGGGCTATTCACGCG	GGTAGCTAAAAAGCGGGTGC	CTCTAGAACGAGGCAAGCCG	AACACGTGCGAGGATGTTCT

 

Amplicon 5'

Amplicon 3'

AGATCTGGGCTATTCACGCGGCACCATCGGCAAAACTGGCACCGGCCGCATCCGATTGCCCCAGGTGGATGAGAAGACCAAGGTGCGCATTAGCAAAACGTTGCACAAGAATCTGCAGAAACAGCAGGTGTACGGCGGCAACACCACGGTCAAACGCCAAATCTCCGGAACGGCATCCAGTGTGGCCTTTACGCCACTGCAAGGTCTAGAGATTGTGAATCCGCAGGCGGCGGAGCGTTCGCAGACGGAGGCCAATGCCAAATACTTTTCAAACACATCCGGATTCATGTCAGTGGGACAGCGGACCACTTAAGAGAACAGCTCTATCCTTATTTTCTATAAAATTAAGCACTTTTATTTGGAGATTTGTCGAATGTATATTGAGTTAGGCGTAGTAAAGCTGCATAAGAGATTCGAATTGTTGTTAGCTGAGCTTGCCGACTTCCAGTTTCCGTTGAATGGCCTCCCACGAGTTGTTCAGCAGCACGGCGAGAATGCCGGCCACCGTGAACACGCCTCCGATGATGGAACAGCAATTCGTGGCGAAGTGGCCAAATGAGCTGGGAATACACAACACAACCAATAAAACTTGTAAAATTAACCATTAGCTTTGTGGCTTACTGACCTGTGTCTCTCCGCATATTTCACCATCAGTGGCGAGAGCTCGTAGCTGAAGAATATCCCCGGCATTCCGCGCTCGCGATCTGAAAGATCTTTTCGGTATCGCGTGACGGAGAATTGATTCGTGTATATGGGCTCGCCGTCGCTGTTTCCTCGCATATATAATGTGGGAACAATTTTTAAATAGTAATTGAACATTTCACTTTTGGTTTCTGCAAAAAGAGGAGAGCAGATTTTCTAGTTTACTTCATTTGTCGTTGATAAATGTATTTAATTTGGTAAGGTAATTACGAATTAAACTTCATGCTTCTAAAAACAGGTATTTCACAATTCGAACAAATGAGTATATTTTTTCCAATTTAAAAAATGAAAATGCCCCACACAACAGACCTCCGAAGTTTCCCAGAAATTCTTCGAGGAGTAAAATATAAATTAAAATGGATGCCTGTGCTTAAATAACTTAGTTTTTTTTTATTTCAGTCTTAAGTATGCTTTTTAATAATATTAATAAATAAAGAATATAAATTGATTTTAAAGCACCCGCTTTTTAGCTACC

CTCTAGAACGAGGCAAGCCGAGCGAAAAACAAAGTGGCAACCCTTCTATAATTTGACAGCTCACGCACACCATCACTCAGCTGCTCACACTCACACGTTTACGAATTATTCGACTAGTCGGTGCCGAAATAAATAGAAATATTTTGAATATTCCTAAAATAAACGAACCTTCTGCGAGATTCATAAGTGCGTCGGAGCAGCGGAGCTTGCTACTCAGCGGTATCAACTCATATAGAGCCGGTGAACCAGGAAAACCACGGACTTAGCGGCATGCCTCTGCCTACGAGCTCATTTTCCCAGCAGGGACCAACGTGCTTCGATAAGATGAAGACGGGCTTCATCATCGGATTCTGCGTGGGCATGGCCAGCGGTGCGGTCTTTGGCGGGTTCTCGGCGCTCAGGTTAGTTGGACTTTGCACTCGGATGCTCCACTTAGCGCTCCTCCAAAGAAGTGACGCAAGTGGAGGCCGGCAATTATGTAATTACTCCGGCTAACTAACGAACCACATTGAAATACACTTTAGATACGGACTACGCGGGCGGGAGCTGATCAACAACGTGGGCAAGACCATGGTCCAGGGCGGCGGAACCTTTGGCACCTTTATGGCCATCGGCACGGGGATTCGCTGCTGATCCTGCTGGCATTTGACTAGAGTTACTAAACAACCACAACCTTAAGCCCTACTCCAATCTTGTTTAAATTACGCTAAGTTATTGCAATAGAGATGTTACAGAATTACGAGTCCGTGTTTGTGTTCACTACTCACCAGCCACGTCGACTCGTAGTCCATCCAAGGGATGCGTCTTGGCGAACTCTATCTTCTCGCCGAACGATAGGTGATTGATTGTGTGCGATAGCTTCACATTGCTGAACTGAAAGTCATGGATGTGGAACTGGCGGATGGAGAAGCTCTTGCCGGGCGCAAAGTGAAAGCTGCCGGCCATTCGATTCACTTCCAGGTGACCCTGGATGCGACAGCCCTCCTTGAAGGCGTCCTCGTCGCTGCGTTTGTACTTGCCCTTACATTGCTCGATCTTGTCCACGGCCACGGTCCATTTGCGCAGCCGGTAGGCGTCGAGAACATCCTCGCACGTGTT

 

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