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CRIMIC CR01898

Gene Dab

CG9695

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3L:16712126..16724575	73C1	FBti0213447	91429

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
part of correct	OK	GCGTCCCAATGGAAATGTCAACACAC	GTCATCGTCGTTATCTGCAGCCTGTA

Images

Construct Design Sequences

sgRNA sequence	TAGTGGTCACTCGTCAGTTTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTCCCAATGAGCAGGAGCAG	TTGGAAGCCGTGGGATTTGG	CTGACGAGTGACCACTACAC	CTTGGCCACGTCAAGACTCA

 

Amplicon 5'

Amplicon 3'

TTCCCAATGAGCAGGAGCAGTACCCACTGAGACTTCGGCCGGGCGAAACATGGTCAAGTCCCTGGTGGCCAAGTTGTCCACAGCCTCCTCCAATCTCTCGTTGGCCAGCACCTTTGGCGGCGGCAGCGGCGCGGCAGAGGAAACGAATTACGCAAAACGTTAGCGAAAACAAACTAAGCAAATACACAAATAACAAACAAACGAACACAGTGGCCCCTCCGCAGTGAGAAAGGGCAAACAAAACCAAGATACAAACATATGCGAAGATCACAGTGAAAATTTTTTTTGATATTGTGCTGATAAGACGAAGCTATATATAAAAAATCAATATTGGTCCTATCACTATAGCAAATTGAGATTTACTAATACAATAAATGCTGATATTCAAAGAATCGAGTTCTTTATAAAAAAATCTGAAGATAAAGAAGATGATCAGAAGAAGTTTTTCAGTGTACATACATGTTTAAAAATCTTTTAAATTCTACTTAGACGTTCAAAACTGTTTTAGCATTCAGTATAAAATTGTAATAAAGATGCTTCGGAAGGCAAAGAAATAATAAAACTGTTTTTAAATCATAGTTCCCAGCGGAAGGAATGAAAAGTGATGCGAAATATTGAATAGAACAATGAAAGTCTTCTTGATCTTGAATGAAGATTTTTAAAATTTCAAATTTGATATACCACAATAAAAAATATATAATCCATATAGATTTTGCATTCCATCAAAGCATCATTTTAAATATTGGATGATCCAACGAGGAACACAAGTGTTCATTCAAGCACATAATTACTCGAAAAATTTGAAAAATGAATCATTCAAAATATTTTAATGGGGATTCGGTCCAATAAACCCAGATTTGTGCACAATCCATAGAGTTTTGTCGAAATGGATTATACTTCTTTTGATAAACTAATGAGTAATTAGTTTGCTTGCCATTTCATCTTATATTGCAAATATTAAAGCATTTTCTTTTTAATTTCGGACTAGATGGTTTAATTATATTATAGTTCCGTTTCCTAAATACTCCTTTTGTAACTTTTTTATGTTTAAATTTTTTGAAATGTTGCCAAAATTTGTAACAGTGCTAAATAAAAACGTTCATAAATGTGGGTTCTCTCAAAATAAATCAGCCACACCCCAAATCCCACGGCTTCCAA

CTGACGAGTGACCACTACACTCTGTAAAACATTTTCGCCAAACCAAAATTATCCGAATACACACCTTAACAAACACAAAGACTCATTTTGGCATTCGATTCTTGGGCTCATTTTCCAGATCGCAATGATCCGGGACGCTTTTTCGGCGATGGCGTTCAGTTCAAGGCAAAGCTCATCGGCATTCTGGAGGTAAGAGTTTACTTTTTGGATAAATTTCAGTATCTAACATTGTGATCTTTCGAAATTTAAGGTGGGCGAGGCCCGAGGTGATCGGATGTGCCAGGAGGCGCTGCAAGATCTCAAAATGGCCATCCGGGCGGCTGGAGAGCACAAGCAGCGGATAACCATCCATGTGACCATCGATGGTCTGCGGCTGAGGGATGAGAAAACGGGTGACTCCCTGTACCATCATCCGGTGCATAAGATCTCCTTCATCGCGCAGGATATGACTGACTCGAGGGCCTTTGGCTATATCTTCGGATCGCCGGACAGTGGTCACCGGTTCTTCGGCATCAAGACGGACAAGGCGGCCAGCCAGGTGGTGCTGGCCATGCGCGATCTCTTCCAGGTAGTTTTCGAGCTAAAGAAAAAGGAGATTGAGATGGCGCGCCAGCAGATCCAGGGTAAGTCCCTGCACGACCATTCCAGCCAGCTGGCCTCCCTGTCGTCGCTGAAGTCCTCCGGCCTGGGTGGCATGGGTCTGGGCCACTCGGATTTGGCCAGCGGAGGAATCTCATCCGGCCATGCCCTCACCCTGCTGGGCAGTAGTCTTAGCACCACGAATGGAACGAGCAGGCTGGGCGTGAGTCTTGACGTGGCCAAG

 

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