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CRIMIC CR01899

Gene CG3376

CG3376

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:24265213..24258013	60C4	FBti0213018	91346

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGGAACCAAACCAAGGAGGAGAACCT	GAATAGGTAGCCGTGAGTATTCCAGC

Images

Construct Design Sequences

sgRNA sequence	AAATAGCTTTAAATATCTAATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GATCATATCGCGCTATGAGA	GGCTTAAAATATGTGATTGAT	TGATCGTCATTTCGCATTCTCAA	CGCATGCATGTGCTTTCCA

 

Amplicon 5'

Amplicon 3'

GATCATATCGCGCTATGAGAGCACAGTGACTGCTCAGTTTTATGGACACACGCACTACGATGAGTTTGAAATGTTCTACGATCCGCACGATCTGAGTAAGACCATATCCCTTGGGCCAATATCACAGGACCCAAATTTACTCCCTCTTTTCAGACCATCCCAACGGCATTGCGTACATAGGTCCCTCTGTATCGCCCTATTACGATCTGAACCCCGGCTATCGTATCTACTACGTGGATGGCGATCATGATGCCACGACGCGCCTGGTCATCGACCACGAGTCCTGGATAATGAACCTCAAGGAGGCCAACCTGTATGGCTACCCCATTTGGTACAAGCTCTACACAGCCCGAGCGGCATATAACATGAAAGCCCTGCGTCCCAGTGATTGGAACAACCTGCTCAACGAGCTGACCAATAATCAGGAGCTATTCGAGCTGTACTACAAGTAGGTGTTTGTGGTCAGATTGAACTAAATGGATTCTATACAGTTATGGGTTTTCTTTCAAGATACTACTGGAAGAACTCCCCGGCGCGACCCACCTGCGATGCGGAGTGCAAGAAGAGGCTCATCTGCGACTGTCGGAGTGGGCGTTCCCACGATCGGAAGCACTTCTGTGCCGAGGTCGAGTCGAAGATCGACGAGGAGTCTTCCAAGTCATGGAAGTCCTGGTTCTACAAGGGATTGACCAATTCGTAAGCACTGTTTATGTCAAGTTCGTGTTTCTATGGTGTAGGTGGCTCACACAAACTAATGCAACTCGAGTTGTAATTGTAATCAATCACATATTTTAAGCC

TGATCGTCATTTCGCATTCTCAAAACATACTATTACTGCATAATAACAACCCCATTTTATTTTTCCCACTGGTGTGGTCCATGCAACAACTAAAAACCTAAAACATATGACCGAACAGCGCCGAGGATCGGACTGAAGAGCCGGCCCCCACCACCGATGGCTATGGGCCTTTCGATGTGGTGATTGTTAACGTTGGTGGTTGATTTACTAAGGAATAAGGTCCCTGTACATATCACTGGTGGCCAGAATCTGTAGTGCCCATGACAATAAATGACCTAAATATTTACCCCCAAACTCTTTCTTCTGCCCAAAAGCTATAGCCTTCTGTCCTCCATCACATACCTGCCCAAATACCTGCTCGGATATCGCTGATTCCGATCTGAGATGGTCGTTTGAGGTCATCCAACTGCAAGTCAATGCAATCGCAACGAAGGAAAACTCCTACCCCATAAGCTTACTATGTGATAACTAGTGCTTAAAACCATTTCGCTTAGTGTACGTTAACGCAATGATGTCCCAAAACTGCCTGCTTTGAAACCTTACAATCGCCCTTCGGAATCTGAGTCAGTGAAGAATAGTCATTTATCTGTAATCGAGTCGATGCTGTTGCCCACTCCGCAGCATTTGCGGTCGTGGCATGTTGCGCGCTTTTACGCAATGGAAATTGTCGAGCAAATTACCTTCTAAGACACCTAATTGTAAAGCTAACCTAGGTCTTAACTTAAATAAAGTGCAATTATTTTTATAAAACATAAGATGTTTCATCTCTTTGGCGTTTGTTTTCCCCGAACAAAAACAATATTCTCCATTAAGATATAAATTCGAATTTAAATTCAAATTTACCAGTGCTGGAAAGCACATGCATGCG

 

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