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CRIMIC CR01902

Gene mop

CG9311

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:14769341..14776129	70F4-70F5	FBti0213448	91430

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTCATGCTCCACAGACTTCTTCATCG	GGCACTAATCTGGCAAAGATGTCTGG

Images

Construct Design Sequences

sgRNA sequence	TTTAAGCCCCCTTTGTCTGGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTCTCCTCGGAATGTTGGCC	GGCGGCTGAGTCATCGTC	AGGGGGCTTAAAAGTTGGTG	GACTTTTAGCTCCACGGCCT

 

Amplicon 5'

Amplicon 3'

TTCTCCTCGGAATGTTGGCCCTGGTATAGGTACAATATGCAGGTGAGATAGTTGATCTTGAAGCGAACGTACTTCTTCCACAGTTTGTACACAGAACTATCGATGACTTGAGCCACTGGACCATCATCTCCTCCCGTAAGAAGAGCAGCCAGTGCAGCTCCATAGTAAACCACAATCTGCGCCGTCACTTTGGCCACAATATGCGGTTTCCTATTGTCGATTAGCGACTTCTCGAGTATGCATTCCTGAGCTTGGGCAAAGCACACCTGTTGCTGAAAGACCAGCAGCTCTGGGGTCATAAAATCCCCTCCGCCATTGACATTGGCATAGCGTTCTCTCAGCTCTCCATAGGCCCAAGCAGCCGCCTGGAAGTGAGTGCAGGCCATCTTCATTCCATCCACATCGCCACGGGTCACCGACGCTCCGGACTGAGTGTGCAATGCGGCAATGTTGAACAGCACCGCAGCCCGTTCGAATCGCAGATCAGTGACCTCGTGAACGGCACTGTGATAGAGATCCTTCCATTTAAAGGTGAAGATTCCGCGATCCGCCAGCTGTGGAAATCGATTCTGAAGGGCGTGTAGCTGGCAATAGTAGCGTTTCATCACCGGCGCTCCATCTTTCGTGGTGTGCATGGCCTGGTTGCGCAAAGTCTCCAGGGCATGGACCTCTTTGGAGTAAGCCTCTGGATCTTCATGATAAAATTCCGCGATGTACTGGAAGTAAAACGTGAAATGTTTTAAGTTGGATTTTTGTTATGGAGTTTAGTGTAGGAAAACAAGAATATATTTTAGGGTAAATGGGTTTTCATGGGGAATTAAGTGCAATAACCCAACAAACTGCCAGTGTTTGCTTATAACTTTTTATTTGTTTGCCAATTTAATCAGATGACGACGATGACTCAGCCGCC

AGGGGGCTTAAAAGTTGGTGAAAACGGAAAATGCAATGGAGCAAATGGCATGGTGAGAATGGGAATTTCGATTCCAATATGAATCCAATAAGCAACGCATTTTCCCAATTTGACCGATAATTCCGTTTCACCAAGTTGTCTCCTCTTTGTTTTGCTGGCTCTCTTAATTATTTCACTCCCCCTTTCGCACTCTCTTCCTTTTACCTTTTTCAAAGCCGCAAAGGAGGTTCCCTCGGGACTCGATTTTAGGGCGAACCAGAGCATATGCAAACGGGGCACCGCCTCCATGGTGATTAGTTTACTTGTTGCCTGCCCAGTTGGCCTTTTATTTTACACACAATTTAAACTAATTGCTGGCAATTAATTTACCAATATCAAAACAAGATTGTTTTATGAAAAATGTTGCAGTGTGGCCGTTTCAGCTTTCTGGAAATATGCACTAAAAATGGCAGGTGCGTAGGGGGTATTCCATTCATTGGCATCGATACTTTTTAGAACGGTATTCTTTTCCAGAAGTATTAAATTTTTATGCCGATTAAATTTTATAAAGAAAATGAAATTTAAAAAATTTGATATTACGAATGCGAATGTCTAACAATTTATATAAATTGTTATAGATGGATGAAAGAAGTGCACTGCATATATATCACTGTGAACGACAGTTTACATAGTGACGAAGCGCACCATATTTTTGTTTGTTTTTTTTTTTCCTTCCTTGAATTCCCCATTAATCACAATTAGTTATTTTATTAATAGTAAATGCGCAACCAGACATTCTGTAAATATTTGTTGTTTATTTTTTTTATTGTTTATACATTATCAACTTAGGCTAAACACAGATGGGGATTTGGATGTAATACAAGTCGTGTGAGGGTGACTAATAACAAGAATGCAATTATTAGCTAATTGCCGCTTACAAACTGACGATGATACAATGGCAGCATACGGCTAAGTTAAGTTAGGTACATACGTTGGTCGTACTAAGACTAATTTAGACTAATGGAAACGCTACTAGGCTTTTCGCTTTGCTACGAGCCGCTTTGGAGATGTTTCGATACAGTTCCTAAGAGGATTGCCCCCTCCCCATTACTAATTACCCCTCAATTTCTATATACATGTCGCTGCTGTGCGTGGAGGCCGTGGAGCTAAAAGTC

 

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