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CRIMIC CR01903

Gene ATP8B

CG14741

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3R:12068375..12090612	87A7-87A8	FBti0213449	91431

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGTACAGTCGTTCCTCATCAACTGGG	TTCCCTTCATGGCTTACCCTGCTTGT

Images

Construct Design Sequences

sgRNA sequence	AAAGCGGCATACATACATGGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TAGTCCTTCGCCGGCAAATT	GCTTTCACCCGACCCGAATT	GGCGGAAACATATAAGCAACAC	TTTCGGGTCCAAAAGGCCAA

 

Amplicon 5'

Amplicon 3'

TAGTCCTTCGCCGGCAAATTTCTATGAGATGGAGTGTGCCACAAATCAAATCGGGGTCAGGAAAATCATTAAGTTCTCTCACATTAAGGTGGTCCTGGGTGCGCGCCTTAAGGTCCTCCTGTCCGCCATGCAGACGATCATATATCACATTGTCCGCTCCTTTGCAGTAGAGAACCATGGAGTCACCGCGCCGGAGAATCACAGACATCCGCTTGCGGACGTTGTTAAAGTCGAGGATATTTAGGAGTTCGTACTCCTATAATATTTTATTTAAATTATGTATATTTTTATATGTATAACAATTACTCACCTCCGTTTGTCCCATCACCTCGATGGTAATACTATTCGGTGTTCGTGTGCGAAAGACAAAGCCAAAATTTCGAGCGGCCGAAACGAGGGCAGCTTCGTCGGGACTTTGGGCCTGGTACTCCAACTTACCATCAACCGTTTCGGCCATGACCGTGTGGCAGAGGGCCAGGAGGCGGAAAAACACATGGGAGTGCTCTTCATCCGACCGGACGGCATCCAGCAACGTGCGATCGTACCAGCGGAAGTCACTCTCGTGATGCGGATTGGCCGAAAAGTCAACACTTTGAAGGGCCTGTGAAAAACAAATAGTTTGCTTTACACTCGGCGAAGATGAAGGCATTTCTGAGTTACTGTACGATAAGCTTGGCTGTATACAATTTTAAGCCAGGTTAGCATAGTGTCCCAAAGTATATAATGCTTAGTAAATTATTGTGGTGTTACAATGGAAGTATTTACGATTCCAAAGCGGAAAATTTTCTCTTAAAAGCGATACGCAACTGGCTTCAACGTTGAAACGTTGATCATGGGTAGAATATGATGTAAAATACGGCCTCATGTTACATAAAGGTAAAAACAAGTAATGAGCGGTACTTTTTACATTATAAACATAAAAACGTTTCTTAAAAAATAAATATTTTTATATTTTATTTGCACAATAACGAATTCACGACTCTGCTGTGGGTATATTGTGTGTATCAGAAAAAAGGGTTGTGTACATCATACTTTCAAAATAATAAATACGTTTTATCGTGTTAAAGCCAAAAAAATTCGGGTCGGGTGAAAGC

GGCGGAAACATATAAGCAACACATAACAAGATACTAATTGATAATAGTGAATGTGAATTGAAGGACATGCAGCATGCATACGTGCACTCTGTGAGGCGACGAACTCCAATTGGTGGCGAATGCCTTGGATCGATGGTGGTGGTGCTTGGTGGTGCTAAGTGTGGATGTGGATTGTGGCTCCTCGTCGAGGTGGTGCTCGAGCTGGGAGATGACAACACCCAGCGTGGCCGTTGATGGCGCTTTTTTAAACCGGATCTTGACTAATCGCTTGCGATTGTGACTTGAATGGTGCATGTTGTGTGTTTCTGTCTGTTGGCTGTCATGCAAAATCTTTACTTTGTCGCAACTGCTAGTGCGGGACAATTGGCGCTGCATACATCCGGCTCCACTCCTTTTGAAGCCGCCACTAATTCGTCGCTCCTCATTGCCTACGGGTGGACTAAGTCCTCCACTTCCATCCAACCTGGGTGACAAACGACCAGCGTCTGCATCCTGACTTCTACTGGGAGCCGAGTATCGCACATGCTTGAGTCCCGGCCGCTTCTCGCTGGCGTCCATCGGCGGAGCAGAGCGCTCGATATCCGATCTGGGTCTGTCTGGCAGCACTTGCGCCTCCCCGCTGGATGTGACCACCATGGAAGTCTTCTTCGCATGGACCTCGGCCTTGTGCACTAGGATGATGGGGGGTGGTGCTGCAGGTGGTGCTACGATAGCTCCACTTAAGGGGCTGGGTCTGTTGTTGGTGTTGCTATTTTGGAAAATTGTTTGCTGCTATAACGAGTAATGTGAGTTGTTGTTTCTTCTCTTTTTTTTTCTTTTCTTTTTTACATTGTCAGACACACAATTAAACAAAGACCCACACGGTTGGCGGGTCAAGGGGGAATGTCATAGTTGAAGTTTGATTTGAAGAAACAGCCTCGTTCGAGACTGAAATTTTTTGGGCAGACTGTGCAAGAAACGGACACCGACACACACAAGACAACATTCCCTCAACCAAGATAGAGGTTAAATTGGCCTTTTGGACCCGAAA

 

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