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CRIMIC CR01904

Gene MED19

CG5546

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:17843725..17846501	75A4		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATCTCGCGTTTTCGTTGAAGCCTCAG	GACCCTACACACGTTACAAGGTTGGT

Images

Construct Design Sequences

sgRNA sequence	AAATCAGAACGCGCCAGTTTAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGCGACATAAGCAGACTCCC	GGCCACTAATTCATATTGCTGCC	TGGCGCGTTCTGATTTATGC	ATCATTCACACGGGGCCATT

 

Amplicon 5'

Amplicon 3'

TGCGACATAAGCAGACTCCCACCGACGCCGCCCCCGCCACCACCTCCTCCTCCGCCGCCGCCACCTGGACCGCCACTAGTGCCCAGCACAGATCCCAGCAGACCGCCACTTGACATCTGCTGTTGCTGCATGGGCATCTGCTGTTGCTGCTGCATCTGCATCGATGAGGAGAAGCTATTCATGTTCCCCACAACGCCAGGTCCCATACTGGCACTAAGGCTGGACAGCCCGGGTCCTCCAGGTCCTCCTCCCAGCGATCCCAGACTAGTGGCTCCCATTACACCCGCTGCTCCGCCCAAGCTCGCTCCTCCGGGCAGCAAGCCCACGCCCGGTTCGGGGCTCTGGTTTTTCTTTTTACGCTTTTTCTCCTTTTTGCGCTTCTTGCGCTCCTTGTCGTCCTCATGGCGTTTCTGCTTCTTGTGCTTTTTCTCGTAAGTCTCCAGGCCGGCCGAGTCTGCAAGATGGTTATCATTGGATTATGTGTGATCGAGTTGCTCGATAATTCAGCAGATTGCTTACCGAGCAGCGTGGATTCCTGTCCCGTGGTCACCCCGTCTTTGTGTTTTTGCTTTTTGTGTTTGTTTTTGTGTTTACGCGCAGGTGTGACATATGTGGTACGATACTGTTCCGGCAGCTGAAAGGAGCACCAGAATCGGATAAATTAGCAATGTGAAAGTGCAGAGGAAAAAAATAATATATTTTATGCAATTTGTTCTAGTAACGATTTATCCATTGTTTGAAATATATGACATGTAAAAAATATTTCACGAAACCTTTTAAGATGAATTATTATTATTATTAGATTGCATATAGGGTGAAAGAAAACTCTCAATAATATTAAATGCCAATATTTCAATAACAAGAAAGGAAGGACAATCCGTCAACAAGGAAGGTTAGTTTTTTTTTTTTTTTTGAATATCATAATATTAAACCATATGGCAGCAATATGAATTAGTGGCC

TGGCGCGTTCTGATTTATGCACTACTTGCAACAAAAATTTAAAACTGAAAGAAAACGGGCAGGCGGACATGGCCTTATCGACTCAGCTGTTCATATAGATCACTGCGTTGCGAACTACTGACTTAATATACACTGTTCAAAAGAGGTTTAAAGGGAAAACCAACATTTACACTCTTGTTAATTTAAGTAATTTTTGTGATTAATGACTAAAATATTTGTGTTTCAAATTTGTTGTTGAACATAAGGAAAATGAATTACAACTCTAAAATACTACGGTGATTATAGAAATAAGTGACAGAAAAGGTGCGACCAAATAACACTTTATAGTAGATCAGTCTGTGCAAGGACACTTAAATCCAAATTTCATATGCAAATTTTAAAAAGCGAAATTAATGCATGCAAATGTATAGAGACGCAAAGCATTGAATAGCGTCTAAATGTATTCTAAGATGTTCAATGTGCCGTATCTAAAGATTCGTCATTTTTCAAACTGAGCAAGCGAATTAAAAGCATATTGGAAAAACCAACCGGTCCGGGATGCAAGCGGAATCCGGCCAGCTGCACGGGAGTCAGCGGAAGAAGCTCCTTTCCACCAATGGGCGGCTTTTCGATCACGCTCCGCAGCGTGCTATTCTCCACGGGATGCGTGATGAGGTCGTTAATGCCGGGGATGTTCTGCAGAAACGAGGCCAGCGACTCCTTGAACTTCTTCTCCTTGAACTTGGTGAGTGTGTGGTGCAGTCCGTACTCGGTCATGAGATCCTTGTCGCCGGTAAGTTCTGCCTTGGCGGGTGGTTCCTTCATGGAGTAAAATGGCCCCGTGTGAATGAT

 

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