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CRIMIC CR01905

Gene CG12502

CG12502

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3L:865396..871701	61D2		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	long band	GCTTCAGCTATTCTCATCGCTGGCAT	CTATTGCTCCTGTATAGCGGATTGGC

Images

Construct Design Sequences

sgRNA sequence	TTCGGAAAGCGAAAAGCGGGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GATGCCACCTACCCTGGATG	AGCGAGAAACAGCCAATCGT	ATCTGAAATCTGAAATCCGCATC	AACACGCCGAGACTCGATAC

 

Amplicon 5'

Amplicon 3'

GATGCCACCTACCCTGGATGCCACCTCACCAGGATACCGCCTCCCCTTCTGCAAACAGCCGTTAGCACCGCACACAGAATGCATTGTGACCTTGCACGGGCAACAGGTGGGGCGATGGAAGTGGGAGTGCGAGTGCCATCTCCACTTTTCCTGTGTGGTTATGCATGAAGAATTCATAGGAGATTTAATAACTTGTGACTTGATTGTTCGATAATCGAGGAACATATATGTCTGAGAATCCCAAAAATCCTCCTTTCCAGCACCCAAATTGATGACAGCCGATTCGAGCCAAGGGAATTGATCAGCAGTCGCGATGCCCTCTCGTCGCGGTGGTGGTGGCACCACCAAGAGAAAGCACTCGCTGGGTAACCTGCGACGGGCTCATCCCGCCTCCGGAGCCACATGGAACGTCCAGGTGGTGCGGGGCAAGATGTCCTCGAAGTGCCTGTGGCACGCCTGTCGAGCCCTGATCCTGGGTCTCACTCTGATGCTCCTCGGCGCCGGCATGGCCACTTTGGGCTACTACGCGGATCACCTTTCGATGGGTTCCGAATTGCGTGGGAATGTCACGGTGCGGGTGAAGAACGACCTCAAGGGCTTCCATCTGAACAACTTCGCGTATGTGGGCCCCATTGTCATGGGTTTCGGAGGCTTCATTGTGGTGGCCTCCTGCGTGATGACCTTCGAGGCACGGGACTCCGCCGCTAAGGTCGTTCCAGCACGGTTGAGAGCTGGAGGAGGCGGCAGCAGCAAGAATCCCTCGCGGAGCAACCAAGGAAGCTCCGCGTCACAGCGTCGCGGGATGGGTAAGTTCTTAAAGTTCACACCATTAAATTTGGGTGAAAGAATAAAGTATTCATAAATATAATTTGATGGATGATCTGTAGGTGCCCAGTACCAGTCGAGTCGTTGGGATCAGCACTTCGGTGTGTTCCGCTCCTCGCCGGGCGATCCTCACCAGCAGCCGCAGACCGCCTGCATCTCCACCACACATCCGCATCAGCATTCGCATCCGCAGCAGCCATTCGATCGCGAGGCACTCACCGCCGAACTGGTCCAGTTCTCCAGGTCACTCAGGTACAGTTGTGGGCTATTTCGGGGAGATCACCTCATTGGGAACAGGACCCAAATTATATGTATATATCAAAATTCACTTATACGATTGGCTGTTTCTCGCT

ATCTGAAATCTGAAATCCGCATCCGCATCCCGCTTCCGCCAACCATCAGTGCATCCAATCGCTTTTGTCCCCAGTGGCGCCGCCTTTCGGGGGCTGGTTCCGCTCCGGATTTGTCCGGATCCCATGCTCATGCTAATACCACCAACTCCACCAATGCCACAAACCACCAAAACCCAACCACCACAGTCACCACTAATTACTACAATTTGCTCTCACTGAATCGATTGTCGCCGTTGGATTGGGAACTGTATTGCTCGCACCGCCGTCACCGTCGTCACCATCACCGCCACTCGCACCACCACCACCACCACCACCACAAATCCTCAAGCAGCAGTGTGCGGCGGGTGCGCCCAGTGCGACCGGGCTTGGGAGCGGGATCGCAACCCTCCGGTGGAGGCGGAGGAGGAGGTGGAGGAGGAGCTGGCTCCTCTTCGTCGGCCCGGATCATCAGCAATTCCTCCAGCCTGATCCAGAGGGCCACCAGGGAGTGCTCCCTGCTCCAGCCACCAGCTGCTAGTCGTGTCTACTGGCAGGCCTCCTCCTTTGACGGCGGCAGTAATCATCCGCCCGGATTGGGCATGAGTTCCGGCGCAGAGAAGCGCAGTGAGCGGAACAAGCGGTCGGACACCGCCAAGCGGCACGTTCTCTCCCGGCAACGACCCATCGAGCAGGAGGAGGCACGGGATCACAGTCGCAGTCCGCTGGGACACAGGCGGTGAGTTCAAGGTGTCCAATAGCTCCTCTAGCTGGCTAATAACATATACCATTGCTTACAGGAACTCTACAATGTCGGACTCCTCGTATAGCGGCCGCTGGACGGCTCGCTGTGCCTCCATGGCCAGTCGAACATCCAGTATCGAGTCTCGGCGTGTT

 

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