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CRIMIC CR02101

Gene 5PtaseI

CG42283

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3R:25040427..25045151	96B17-96B18	FBti0213450	91432

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTACCGTCTCACGTGCTGTTCAGTAT	GAACTTGAGTTGATGGTCGGCATGAC

Images

Construct Design Sequences

sgRNA sequence	TGCTGAATACCAATTTGTTTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACAACGCCTTCAGTGTCACA	ATTCAGCGCCCGCCGATAAT	TGGGAACTCGGATTCGGATAT	ATTTCGGACAAGTGTTGCGG

 

Amplicon 5'

Amplicon 3'

ACAACGCCTTCAGTGTCACATCTAAAATTAAAATCCCCAAAGAGGAAAAATGGCACAGTGCCATTCTGTTCGTCCAAATGGAACCTATTCAGTGTATATTAACTTATTATTAGCTAAAAGATTAGGCAGCAGCAACAGTCACCTTTCAATGGTATGAACTAGGGCTCTTCTGCGCGTCTTACAATAAACGGAGGGGAAATTTTCGCATGCAGCCAAATTGGATGCATCGTGGAATAAGTGAATGTTGACTAGATCGATGACGGTGCCATTGATTTCCCATCGGGTTCGCATAAAACCTTTTCGGGACCATTTACACTGAATAGAATATAGTAAGAATATGAACATAACTTCATGAATTGCATCAAACCTACCTCCGGAAAGAAGTGCTGTGGGAACTTGGACTTTTCTTTGGTAGCAATAGTTTCAATATTTCCGGAATAGATATGTTTATCCTTAACATCTTGAAGACTTTCTTCCCAGCTGTGAGTTAGGAAGTTCCAGATCTTCAGAGAAGCAACATCCTTATGCACAAAGTAAAGACTTCCAAGGGCCTAAAAATAATAATACAATAATGTGGGTATTTGTTTTAACCGGATATAACATTTTTGTATGTTGATATGTGAATTCAAGAGACTCATTCATCATGACTAAGCCCAGTGACTTAATTCATATTTTTTAAAAGGCTTTCGTCGCAATTTTAATTGAACAATAAGTGCGTAACGCCTCAGTTCATCCGAATTGGGTTTTTATGTGCCGATTGACCTAGAAACACGATTCACCCACCCACCGTAAAGTGCTCGGCCGATTTGAAGTCCTCGTCCATGAAGATGTGTACGCAGGTGAAGTCGCCCATTTCGGGAGCATCGCAGAGGCACCTGATGAACTCCTGGACGTACTCCATGGACTTCTCGTACGTCTTGCCGCCCACCTCCTGTAGGTGGAGGGCCAGGAAACGGGGCTGTGCGACGGCGATCTTTGCAAGAAATTCGCTTAGCCATAGCTGCAGAAGCCTCTCCGGCTGCAAATTGGATTGGAAATTCGATTATCGGCGGGCGCTGAAT

TGGGAACTCGGATTCGGATATACATATCTATGTATGTCACTGGGCCCATACATAAATATGCGATTTACGCATCGTGTATGTATGGGCCGTACGTAAACATGTATACTTCCTAGCCCTGTGGACATGGCGCATCCGTTCCAACGGCCACTTACGTCCTCAAATAGGCTGCCAACGTTTGCCGTGACCAGAAACACATCCGTGCTGGACGCGTCTTCCTGTGCTGACTCCATTTGATCCCACTTTCGCCTGCGATGCAACAATTTTATTCACTTTTTATCAGCGTTCCAATTGTAAATACTAATTGAGTCAAGGCGCAGTGTGACCATACCGCCTGGCGAAAACTAGGCCATTTCGATTATTAGCTTTTAAGTGACCGTAATCGTTTATATTTCAAATATATTTATGCATATCTCTGCAGTTTTTTAATTAAACTTATTTATTTCGTTTACATTGAATAATACAATACCCCTCACGAATTTAAATAGTTAAGCTTCATTTCTTTCATATTTTGTTAACTTTAGGTTTCAGAATCGTAATTAGGTCACTTCCGGCAGTGACCGAGATAAAAGTAACGGTAATATCATGAGTACAATGGCCAGTTAAAAGTAGATATAAACAATTAAATGGCACAGGGAATAGAAATTAATGGGTTTGGACAAAGCAATAACATTGAAACTTGCAGGTTTCTAATGTTTTACAGTTCTTTGATCCTTAATATTATTATTTATCCTATTATCTTGACTAAGTTTTTGAATTTCAGTGTAACTGCAATTAAATGCGTATAAATGCAGCTCGAAATTAACAATTCATGCGGTGTGGTGGCACATTAAGCAATTTGGTGATCCGTTGTCTATATTATAAGCATATCCGCCGACATAAAACTATTAAGGCCCATTAAGACCTACGGTGTACATCATCTTTTCATTCAAACAGCTTACAGTAGTTACTGCGTACTGCGTATGACGGTTTCAATCTTTGACAGAATTCCTAAGTATCTTCAAAAGTATTACAACCGCAACACTTGTCCGAAAT

 

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