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CRIMIC CR02102

Gene CG10038

CG10038

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3R:12350644..12348987	87B7	FBti0213451	91433

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCCCTGCGACGTAAGTGGATCAGAAA	AAGTTGACAGCCCTTCCTTATCCG

Images

Construct Design Sequences

sgRNA sequence	GGCATATGTAATAATATTTGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGATGGCCGAGTAAGAGGTC	CCACGTGGGTAGGAATAAGCT	GTGGAAAGGAATTGTGTGGTCT	AACCCTATGCCTTCACTCGC

 

Amplicon 5'

Amplicon 3'

CGATGGCCGAGTAAGAGGTCCATTCGTGCTTAGTGTGGCCAGCGGAAATCCTCCGGATGCTGCTCTTTGACTGTGAAACGGGTGTGTCTAAGGGTGTGTTGCTGGCAACCCAATCGCAGGCCACATCGCACAGGTACTCCTTGTTCTTCGCTTGGGGACTTCCCATGGCGGCATCAGTGAAGGCAATGGCAAAAACGGATTCTTTAAAGAAATCCGGAAACCGCTCCACCTAATAAAGAGAAGATTAAAATATGATGTTGAAATATATTTGGAATTTTATACCCACCAAATCAAGAGTAAGACTGCCACCATAACTGTGGGCCACAATGGCCACACTCTCGGGTTTCGCGGGCAATACAATATTCTTCCACACATATTTAACGTGCTCTACGGACGTATTGACGCCCTTGATGGGATTCTCCTTACCATTGTAGAATCGTGTGCAATCGTTGGCATTGGTGATGAGGAGATCGTATCCCAGTTTTTGGGCCTGTCGCACATAAGGCAGCTGGGTACCGTGGTCCAAGGAATTGTTAATAATTAAACTATAACAAAATTAAATATGTAAGTATTTGTATTTGTATGTTAATATCATTTGTATTGGTATTCAGGTATTTTTATCATTTCTATTATTTGGTTTAAATAGTGAACATACACACCTTCTGGCCCACTGTCCGGCTTTAACGTATCCACTGCCATGGATGAGGACTAGCAACTTTTTGGACTGCGACAGCTTTGCCGGTTGCGAGAATACAAACGAAGACCGCTCTGGAGGCTGGTCAAAAGGGATGTAGGTGCGACTAAGACCGTTTTTCTCCAGCAAAGCGTAGACAATTTCTGGGATTTGCTATGGGCGATAAAGGATACAGTATTTGTATAAGCTTATTCCTACCCACGTGG

GTGGAAAGGAATTGTGTGGTCTAGTTTCTCTTGAAGAGGATTCGTCCTAGGTGTGGATTCCTTGACATCAAGGAAACCTAATAGATTATAGTTTTCGGCAGAAGATATTCATGTGCAATGTGGGTTGAATGCGGTACATACGTTGGCTAGCTTCTGGTAGTGCTTCTCGTTTTCGGCGGCGTCGTCGCTGACGTTGTAGGAAAAGAGTTCCTTGCCAGGCTCCCCAGTGGCGGGATCGACTTTGCGCAGCACTCCTTCTGCAGGAGGCAAACGGGAAGATCATATTGTCAAGTAGCGACTTTGTGCAACATTCTACTTACCTCCATCGAATGCATATCCAAAGTTTTTCAGCTGGATGAGAGCCTCCTCGCAATTGCTAGCCGGCGGCGGTGAGTGCGACATTCGAAAACTTCTGAGGGGTCGAAATATTTGAATAAGCCTAGACCACATTACAATCGGTCGCGTGGCTTCCACACGGTTCGGCACTTACGTAGTAATAGAGCGATTGGAAAAAGCTTTTATTTATATAGAATTGTTGTTCGAGTTTTTGTTTTGATTTTTCATATGAACGCCGAACTAGGGATGGAATAAGCGCGCCACCTACTGGCCGCAAGCTCACATCGTCTAAGCGATAGGTATCGATAGCGAGGTCAGAGCACCAATTCACACCAAAACGCATCACTGCCAAAAAAAATCCCCATAATATTCGTTATAAATTAAATTTTATTAAATAACATTTTTTAATATAATATTGATCCTTTCCTGATGATTAATCATTGTCTTTTAATTGTAACCCTAGATAGAAAAGATCATACATATTTCTGTAAAACCTGCTATAACCCATCTTTCCATACAGTGCGATTAATTTGTAAGACTTTTAATCTCGCAGCCATAGAGTCCATGGATTCCTGAATCCAGCTGGAGTATGCGTTGATGCGAGTGAAGGCATAGGGTT

 

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