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CRIMIC CR02103

Gene CG1129

CG1129

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3R:4816025..4811444	82D6	FBti0213452	91434

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GACAAAGTGCAGTGCGAACATCGGAA	CGCAGCGAGCTTAGATACAGACACAT

Images

Construct Design Sequences

sgRNA sequence	GTAACCGTCTTCCCTAAATTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACCGGAACATGCCTGATTGT	GGCAACGGCAACCAATATGG	AGGGAAGACGGTTACATAACAC	ACTTGGTTTCTCCCATCGCC

 

Amplicon 5'

Amplicon 3'

ACCGGAACATGCCTGATTGTGGTCTTTAGATAGGTTATTTAATTTAAATTAAACTGAAAAGTATTTTTTAAGATTCTTCAATTTAACAAGAAATGAATTTTAATAAATTTTATTAATTTAGCGTAAAATGTTCGGTGGAATCCGGCTATCAGCGCTGCTGCGAGTGGGACGAAGCGCAATCATCGCCATCTTCCGATAGTGTTATCCCAACTTTGCCAGCTCGGTTTTTATTTACTTTCCTGTGCGGCGAGTTTACTCATAAAACAAATGATAATAAAATACACAAAGACCATATTGAATGTTCAATGACAAGATCGATATTCCTAAGCCTTGAAAACGTCCATCTAAAACTAATAGAAACTATTATTGTTTTGGGACAGGCAAGTGCATCCAACCAGAAGCACAAAGGTTCTTATCACGAAAAAAGACAATTCCAAAAACAACAACAAGGAAATAGATAGCGCAACAAGGTGAGTAAACAATAAACTAAAGACAACCAACTGAGTCACTCACTTGCCTTCGTCGATTGCAGTTGTTTGACCGCTGAAACGGAATCTCGTTGTACGGAGAAAGTCTCGAGAACTTTAAGGTGTGACGTGTCGCTCGCTTACGGACTATAGAAACCGGAGAACGGAAAGGAGGCGGAAGCGGAAGCGGAGAAGTGATGTGTGGAAATCCAGCTGTGGGAAACGGGACGAGGGCGTTGATTCTGGTCGGTGGTTATGGGACCCGGCTGCGACCCCTGACCCTCAGCACGCCCAAGCCACTGGTGGAGTTTGCCAATAAGCCGATTCTTCTCCACCAACTGGAGGCACTCGTCGATGCGGGATGTCGTCAGGTATATCCAGTTTTTTAGACTAATGACGCCCAAACTGCCCCATTGCTTAGGAGAATGGAAATATGGCACATCATTCTTAAAACAAGGTTTTTTAAACACCTTTGGATGTTTAGAAAACAGTTGATCTTGTTCCATATTGGTTGCCGTTGCC

AGGGAAGACGGTTACATAACACGCTAAGAATCAAATCAACTTCCTCTAAAAGAGATTATTCTTTTGTTTTATATTTCTAAAATAATGCATACTTCTTTCTCGCAGGTTATTTTAGCCGTCAGCTATCGAGCCGAGCAAATGGAAAAGGAGCTTAAAGTCGAAGCCAAGAAACTGGGCGTGGAATTGATCTTCTCACACGAGACGGAACCCCTGGGAACAGCTGGACCTCTGGCCCTAGCAAAAACGATTTTAGCAGCCAGCTCAGAGCCATTTTTCGTGCTCAATTCGGACGTTATATGCGATTTTCCATTTAAACAACTAGTGCAATTCCATTGTAATCACGGAAAAGAAGGCACAATTGTTGTCACAAAGGTCGAAGAACCATCCAAATACGGAGTTGTGCTGTACGATGAGAACGGCTGTATAAAAAACTTTATTGAAAAGCCACAAGAGTTCGTTAGCAATAAGATCAATGCCGGCATTTACATCTTTAATCCCTCCGTGCTTGACCGGATCGAAGTTAAGCCCACATCAATAGAGAAGGAGGTGTTCCCTGAAATGACGCAGCAACAGGAGTTGTATGCAATGGATTTAACTGGATTCTGGATGGACATCGGACAGCCAAAAGACTTCCTAACCGGTACGACAGACAGAATCACTCGTTTGCTCGCTCAATCTTGATTGCCTAATTGGTCTTATCGCTTTGAAAGCAAATGTGGATGTCGTTGTTAATTGTGCTGTGCCTTTTTACCCACCCGGCTTTCGGCTTTATCTATTATTGACATTCCTGGAACTATAGCTAATGTTTGATAGAACGATTTCTTTTTAAATGAAGTGAGGCTCATTAGGCCGATAGTGGGCGATGGGAGAAACCAAGT

 

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