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CRIMIC CR02105

Gene CG14880

CG14880

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3R:16360329..16368306	89B13	FBti0213453	91435

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAACCTTCCGAACTTCCCAAGTCACT	GAGTGGTTGTGGTGGTGCTAGTGATA

Images

Construct Design Sequences

sgRNA sequence	CCCAGACCGCAGGTTGGATTCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCGCATAAGGACGATCCAGA	CCTGACTTGGGCCCAAAGT	CCCAACTCCCAGACTGAAGC	GGGCGCAGAATGTGTGTTTT

 

Amplicon 5'

Amplicon 3'

GCGCATAAGGACGATCCAGAAGATCGGGTCTTGGAGTGCGCAAGCTGTTTTCCTCGTCGTTTACGAAGGGCTTGTCCCGCTGACCTGTGAAATAGATTTTGTAGTCAAATCTAATATGATACGTTAAGATATAATACAAACCAATTAGGAGATTGGCAGCATAGTTGCTCTCCGCCTTGGAGCAGTTCACCATGTACCAGTGGTCGCAGATAAGCATTCGCTGCTGGAAAAGTGTCGTGTTTGGGCAGGTGTAGCTGAACTGGCGACCCAGGCCATCGCACATGTGGTACACCTGGCAGCCCGTCTCCACATCCGCATAATATCCGGCTGGACGTCCGGCGCAGGAGAAGGATGTCTTCTGTATCAGCAGCGAGGCAGGATTCGTTTTTGGGATGGCATTTTGAGCCTGCAGGGAATTATAATAATCATAATTATTTCAATTAAAAAGATAATATTTATATTTAATTACTACAACCTTTAATTAATTTCTTAATTATGAATGCGTTTATAAATACTAAGAATGAAACATTTGATTCACATCTTAAACAAAACATTCTGGTCAGTAAGTCTCAACAGAAACTTCCATTGCGAGTTGTTTTGGATTTCTATTTGGCTTGTCCAATAGACCTTACACAACCTTTTTTGTTGAGAGATCCATGATTTCATAGATTGTTCGGAATGCTGGGAAAATATTTTTCACGGACTGAGCGGAAGCTTCTGCACATGTACATACATAGGTGTAGATCCAGTGGCAGATTTCGATAAGTCATTGGGCTATAATTTGCTACTGTCGACGAGAAATTTTTTCATTATAATTAGACACATCACAGGGAAACAACAACAATTTCCACAAAAACCGACTAAAAATAAAGTTGACATTTCACACAGCAGCACAGGTCGACTGATGCAAGAAGCCGTGGCACCATGTGCCAAGTTATGACATCGCGTCAATTCATCACTTTGGGCCCAAGTCAGG

CCCAACTCCCAGACTGAAGCCCAAAATGTACACCTTATGCTTACAGCATTCACATTCCCATTCACATTCACACATGCTGGGCACGAGTATTCGCCGATATCGATCTTGGTGTCATTTATTTGGCTACTGCGAAAGTCTTGGGACTCCAAAAGCCTCCATCAAAAGTGGCCGATTACAGATTATATTCGGAAGCCTTCTTATCTCCCAATTATATCTCATAGATGCGAGTTCCTTTCACTCTGTCAAAGTTGAGTAACTTATGTTTAAATTTGAATTAAACTTAAGAATGTTTGTTCATAGTAAATAATTATTTAATTATGCAAGTAAAACTGCAAGAGACTGAAATTATAACCATAATATCTAATAAACAAAGCTATTGGCAATAAGATTTCAGGCCTATTCATTTAATTTTCGTTACTCGTAATAGGCTATAAAAAGGGTTTAGGTAATGTGGTCTTTTAAATACACATACGAAGATCGGTAATTGTATTGTCTCTAAATAAATTGAAGTCAATTTAAGTAGTTGGGCTTGAATGGGTTACTTATAAGGAGAGATGGTCCCCTCATACTAGAAGCTCTTTAAACCCAATTGATATTTCCCTTAGATGGAAAGCTCAATAGCTCCACAAATTGCAGGAGCTGGGGGGGAACTATCTGGAGTGTAATCATTCCTAATACGACAGGGAATCACTTAAGGAAACCCGATTGTGCAATCCCCTAGGCAGGCACAAGACACAATCCAAGGCCCCGTCTAGATTTCCATTTCACGAGACGGAGCTGTTTGTGTTTCTCATTTCGGGCAATTTGCCATAATTTGAAACGCTCTTCAATTTCATTTCGGGCATATTTACGGGCCGCACTAAAACACACATTCTGCGCCC

 

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