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CRIMIC CR02107

Gene CG15537

CG15537

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3R:30435177..30443599	99F4	FBti0213454	91436

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CATCAAGTATACGCATCGTGTGCCCA	AACTGAAGGAGAAACGAGTCCCTCGA

Images

Construct Design Sequences

sgRNA sequence	GTGGGCAGAGTCGTGAAGCCAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCTGACGTCATGCCTTTGCT	AGGACGTTGGCAATCCACTT	TTCACGACTCTGCCCACAAA	CGTCCAGGTGAGTGTCGATT

 

Amplicon 5'

Amplicon 3'

TCTGACGTCATGCCTTTGCTTTTTGAATCACACATAAGCTGCAAGGGAATATAAAGCGGGAAATTCGAGTGTTGCCACGGCGGAAATTAGTTCTGCCTTAAATGCCTTGGCTTTCCCCTGCTCCCCGATTTATGTCGAACATATTGCATTCGAGATTTATGCACGAGTTCTGCCCGAACGGAATTGAATAGATGGCATAAATTTCGCGCAACTGCTGCCACATGATGAAGGAAATTTATGGAAATTAGAGGGATCCCACGAAGATATTGGGGCATAAGATATGAGGCCTTCTAATCCAGACTCAATAACAGCTCACAAAGGCCCGAAAACATTATAGGGGTAGATAGAACTTGATCTTAAAGTCCAATCACATCATGTTGCATACTTCACGTTACGTACCTTCATAAGTGAACTCCCTTGTAAGCGTAAGTATTCATGTTCGAAAACTGAGTAGAACAAAGCGGTATATTCCATTCAGGACATTCTCCTAAGCACCCAATTTGTGGCTGGTGCAATTAGGGGTCACCTCCGAACGCTTAAGCTTATCTTACCATGTCGGTCCGCTCCACAACTCGCTTTCCCTCTGTTGCAACCGATGACATTCATGTTTTAATTGTTGTCGTTCTCACATGTTGCCAGAGTGAAAAACGAAGCTGCACGGGAGGAAATAGAGGAAAAACAGGAAAATGCGGACGGAAATGGGACAGCAGGGGTGTGGCTGTCATGTCCTGGCTTAAATGGCTGTAAAGTGCCAAAGCGTCGGTGTCAACATTAAGCGCACATCAAGTGCAGGACTCCCAGGCAATCGAGCAGGATGTGGATTTGCGCAAGTGGCAGAGGGGGCGGAAGGGGCGGAAGGTTGGCACCGAGTGTTGAAAATCTCGTCCATGTCCAAAGTGAGTGACCACGGAAGGAATGAGTCAGTTGCAGTTTCATTGCCTTCGATTCGCTGGGCTTTCCGCCCATGACTGTTTGGTTTTTGCCACCGCCCACGGCGCAATGGCAGGTGCGAAATTTACATTTATCCTTGGCCAGTGAAGTGGATTGCCAACGTCCT

TTCACGACTCTGCCCACAAAGTTGCCATTAAACAAACATATCGCAGGTTCTTCAGGTGATGGATGGCAGGAGCCAGGAGTGAGAAGTAGAACCCTAATCGTCCAGCAGTTTCCAACATTTTCAAATGCCTTCCGTCTTTGGCAGGCTAACATGACAAATGTGGTTATACTAATATTTGAATATCCTTTTTGCATGTGTAATATTGTTTTCATTTCTGATCGCAATAACTTACATTACTCAGAGATGAATCATATTTCAGTGCTGTTCTACTGCTGTTCTGTGAAATTAAATTCCCAATCATTTTTCACCCTTTTCCATTTATCTCAACCACGCTCCTGAAAAGATGGATTCGATTCGGCTATTTCAGGCGCTGCTATCTTATCTGTCTTGGAGCAGTCTCAATCATAATTAGCTGGTTAGCCAGCAGAGGTTCTATTTTTGTTTCGGCTGCAACAAATGTCACGGGAACTTTTCCACTGATTGGGCCATTACCAAATCGAATTAAATATTCATTTGCGACGTGCTACTATGCTACTTTGACCCGCTGACCCGCTTGGACTTCCATAAATTGATTACCAACTAGAGTTTGCAACGTGAGCCTAATGAAGGTAATCAGTTCTAGCATAAGCCAGAACTGAAAGGCTGCCGAAAAGTTTTGCAATAACGCCGACAGCTAGAATCAATCAATCTGGCCAAAAAAATCTGGGCCGAGAGAAATTAGATCAAGTCATTAGAACAACGAGGAAACAAAAGTTGGCCCAACTTGTTGATCGAATTACAAGGAGTTACACGCGTGACTTCGTCTTCTATTGATACACAGGGAATAAAAATGTATATTTATGAGACATTTTTCTGGCTTACAGATGGCTTCCAATGGAATAAACTAGCACTTTTTAAAAACAATAGTTTTGTTCAACTCACGAAAGGCAAATATTTATGTTTAGAAATATAATTATGTGGTGATTAATATTTTTCTGTGTTACATCTTTCAATCGACACTCACCTGGACG

 

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