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CRIMIC CR02108

Gene CG4362

CG4362

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3R:20306216..20307537	92E2	FBti0213455	91437

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
long band	long band	TCTGGAAGACTCGACTACCAGATGTC	ATAGGAAGCAGCTCCAAGGACCCAAT

Images

Construct Design Sequences

sgRNA sequence	ACGATAATTGAAGATTAGTGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGACCCCAGTAGATGCTCAC	TGGCACATGCATGGGATTCT	TCGTAGATCCTTGTGGTTATCC	CCCGGTTCGACCATCTGATT

 

Amplicon 5'

Amplicon 3'

CGACCCCAGTAGATGCTCACATCGGCGCAGTTCTTGAATGTTTCCTGTGGGCCACAGCCCAAGGCTCCGTTTCCGGAATTATCGCAGATGCCCCAGTTGTTGGCTGAAATGCACAAGATGTTCAATTAATTTTCACTTTAAGTTCCATTTACGTTCTGGCCACCACTTACCACCCACATAGGTCCAGCGGAGGACGCAGTGAGAGCAGGTAAGACCCTCCGGCAGGACGACTGTCACATCGAATTCGCCCATCTGATCGCCGATATCCTTTCTATCGCTTCCATCGATGAATCGCAGGCGGTTCTGATCGAAGCACTCCTCCGACTCGGCACCGAAGGCGTCCAGGTTGCACAGGTGGAACTCGAAGTAACCCAAGTGATTGGTGGTGATCTTCACGCCCACGGTGATGGTATTGCCAGCCACGTAGCTCCTGGTCACCACACCAGCTCCACCGATGCTTCCTCCGATCTCGTTGGCACGGGGCTGGGCATCCAGGAAGTTGTCGCCGCACAGACCACAGCGACCTCCATTGTTGGACTGCGTCTGCGGGTTTACGAAAACATATTTGATGGTAAACGTTTTGAAGGTCCTCGTTTATAAAGAATACGACATCTTTTCGTACCGTTGAAATCAGATTAAATAATGGCATACCATAAGAAAGAATACTATTGATGTCCCTAGAACATGATTAACCAACACTGGAATAGGTTTCTAATTTAGTGTTGGTATTCCATATTTGATTTCCATTGAAGATGCCACTTTAAGAATGCGCCCCGAATAATTTGGTAATGAATCGTTTAAGAATAACAAATTTTAGTTTCTAACATAAAGCCTTGGCTAAAAGTTTTGCCATTTGCTTTAGCCCACTGAAGAGCATCACCATTAAGAATCCCATGCATGTGCCA

TCGTAGATCCTTGTGGTTATCCAATTATCCAATTGATCCGACACTTACGTACAAACCGCCGCAGAAGAGTTCGTTGTCGTTCCAGTTCTGGGGTGCGGATCCATCGTAGCGCCAACGGGAGGATCGGCTCGGCGGGGACAGCATCATGCCGTGGGCGTCGATCTGCTGCAGCCCGATGGCCAGCAGCAAGGTCATGCTGAGCAGGTACTTCATGGTGTATATTTGACTTTCACTTTCACAATGCCACCCGCACGGACGCACTAGGTTTTATATGTGCTCCTGTCCGACTTATCAAACCCATTGGGATCGAGAGTTTTACAGTAGCTCTCATGGCGATAAGAACGTGTGATAACACTCAGTATAAATAATAACTGTTGCAGTTCCTCCAACCGCACGAAATCTGCAGAATCAACACTCCAGATAGGCCCGGTAGGATAACCATCTTCGAGTGGCATAATGAAATATTCATAAAAACGTCGGTCAACCCTAAAGATCGTAAGTGGCCCGAATAGATACTGTTGAGTTCCTAATCATTCTCGTGCAATCTGAAGTTCGTTTATTGGATTAAGCGATCTCGAGCAATGAGCAATACAATACATTGATTGTTTGTTTTGGGTTGCCGGCTTATCGGTCGGCGATTTCTTTTTAAAATAAATTCCTTAATTTTTGTTGATAGCAAAAACATCTGCTTTAACCTACTCCTATAAACTTTCCATGATAGCAGTATCGCAGTTTTGCAAATATTTTTAACTTTAATTTATGGTCGTACACGAGTAATTCATTTATTGCTTCAGAAATAAAGTTGTTAATTCATTAAAATTAATTATCGAAAACGACCGATCCATATTTCCCATGACATCTTCTAGATTCTGGTGCGCAATGTTAAGATATTGCATGGGTTCAATCCAAATGTTATATACAAACCATAATTGTAACAGTTTTGTGGTCAGCTATAATCCCCATTTAAGGTAATACAAGCAGATTAAAAATAGATCTAGAGTTTCGAATCAGATGGTCGAACCGGG

 

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