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CRIMIC CR02110

Gene CG5447

CG5447

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3R:26452534..26454585	97B3	FBti0213456	91438

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	long band	AGTTTGAGCTGGTTGTTTCGGAGGAC	TTGTGGTGTTGGTGGATGTGGTGGAA

Images

Construct Design Sequences

sgRNA sequence	TGTTATACGAGTTTAAAATAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGGAACGATAGGGATGGGGT	AACTCGTATAACAGATAGCACGA	ATAAGGCAGTGGCACTTCCA	TTTGCACGCCACATTTTGCT

 

Amplicon 5'

Amplicon 3'

TGGAACGATAGGGATGGGGTAACACTATTTTGGTATAAAAGCCAACTCGCCATCTGGTAACACTTGGAGTTTGCAAACATTTATCTCTCGACCAATTTAAGGAATTTGAGGCAATTAGGGCATTCCTGTGAGCTTTTCCAGGCCAACTGCGATTACTTCAAGCCAAGACAAACAGGTGGGTAACCAAGCAGGCCGTGCCCCACCGACCAGACCAGCAAAACACCCCCATTTCGGGCGGTGCTGGAAAAAGCAAGGCCAGCTGTCCATTTGGGATTTGGACACTCCACTCTGAGCATCAAGATGATTGCCCTTAACCAAATGCATCGGGCGCTGCAGAGCGAGGCCATTAATGCCATTATCCGCCTTTTCAGCGACAACATGTCCCAAGGCGGTGGCACTACTCCAGCGGGCGGTAATCCCACAGCGCTCCAGGCGACCGGAGGGGGCGTGGTCTTCAGCAAGGTCTTCATCCAGCGCGATTACAGCGAGGGCACCTCCGTCAAGTTCCATACACGGCTGCCCGCTGAGCTGGAGGGCATGGTGACTATATAATACTCTATATTATCTGTGGGATTATCATCCGAACTCATATCGATTCTCGTGCAGATTGAAAGACACGTCTTCGAGGCCACCATCAATAGGCTAAACGAGTTCTACGCCGAGGCGGAGGAGGGATCCTGCGGCACCTATTGCGAGGGCTGCATTGGCTGCATCACGGCCTACTTGATCTATATGTGCTCCGAAACGCACTATGAAAAGGTGCGTCATTTGGGTTTGGATTACACACATGTGGGTCAATGCAATAGAGTCTAGTTCCACTAGGGAACTATTAATAGGGATTAGAGTCAACAATTTCACAAATGTCGATATGCGATCGTATAAGTTATCAAAGTTATTCTCGTTAATGTCCTTGTACTCAAGGTTTTCATATCTGTTTAAGGTATAAAGGTCAGAGAACTTGATAAATCAAGTAGTAGTATTACTTGCTAATTAGTGATCGTGCTATCTGTTATACGAGTT

ATAAGGCAGTGGCACTTCCAGCTCTATTGCCTGCACATACTAATGCTCTTTCGCCCCCACAGACCCTTCGCAAGATATCCAAATTTGTGGCTTCCCAAAATGAACGCATTTACAACACCAAGGGACTGCAGCTGATCGATCCCACTTATCGGGGACTTCGCGTCATAGAGATTACAATATTCGATCGTCCGGGGCGAACGTGACTTCCGCTTTCCCATTCAGCCAACGAGTTTTTCATGTGAGATCGAGCAGCTACAGTCGCATCAACAGGCATGTGAAGGAGAAGAGTCCGTTTCAGCAATTATCAAGCAAAAGTCAGCGCCATCAGAGTGCGTTTCCAAAAATATAAATCAATAAAAAGCTGTGGAAGCAGCTAAAGCTTTATAACCTATAACCATATGTGTGAGTATCTCAGATATTCCGCACTCAATTGCAAACTGAACTAACTAACATTTACTTTCGCATCCTTTCAGCTTGGCAAAAGCAGTGGCCAGAGCGTTTTCGGGGCATGCGACTTAGTCACGGGTGTGCTGGTAGATGTGCCTCCGCTGCAGCCACATACTGTATGCTAGATTAGCCGTTAATGATTAGCCAAGGCAGTGTACGGCCAGTTAGTTAGTATTTATGCAATAGCAGGCATAGTCATGTAAGTGAAAAGCTACCGAAACTGCATTTTGAGCCGAAGCAAGAAGCATCAACATATACACACATTCGCATATGTTACAATTGTTTTATTAATCGGACCGAAAGGCCAGCGAGAATTGATTGTTTTGTAATTTGAAAATGAATAGAATTGTATACATCATGTTTGTAGTAGTTGCCAACAATTCGATTGTCTAATGTTGTAATTTCAATATGGCATTAACATTTGCATAAATACACACACACATAGTTCAAGCTTAATCAGACATAATGCATACGCATAACTTTTTCTATACAATACTTACCGATTTCAAACAGCGCCAAATGAACTTACACATAAATCGAATCGAGAATCGTTCGATATTTAATATAAATCAGCAACATTGATTAACATAGTTATATATCTTATAAGAAAAATATATATATTGCCGTCATTTAAACGCGCCTACAACTGTTTTCCTGCGGCGGGTAAACATCGAATTGGAAGCAAACAAGTTAAGCAAAATGTGGCGTGCAAA

 

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