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CRIMIC CR02112

Gene tsl

CG6705

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3R:21778114..21782536	93F9-93F10	FBti0213457	91439

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGCCTCGCCAAGCTGTTAGAATGTGT	CTGGTTGAAAGCCTTGATGAAACGGG

Images

Construct Design Sequences

sgRNA sequence	GCTCATTCTAACGACAAACGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CAGCGACTTGAGGTCCAACT	TTGTCGTTAGAATGAGCGCT	ACGTGGCGGAAGTGAACAA	ATCGCCCGTTTATGGAGTGG

 

Amplicon 5'

Amplicon 3'

CAGCGACTTGAGGTCCAACTGCAGTATGGCATCGTTGCCGAGCAGCGAGTCCAGGGATCGCAGTAGAGTACTATTACCGTGCAGTTTCAGCAGGGTCACATACTTGACCACATAGTACTCGTACTGCAGCTTCCTTCTCGCCCATCTTTCCACTGTAGCATTGGCACTAGCCGATCGAATCTGGCCCAGATGGGCGGCAAACCAGGGCGCAAAGTAGTTGTAAAGATCCAACTTGGACAGTCCGTTCAGACCTTTACTCTTGATTCGCTCCTTGATCATGCTGTAGTTCTTCCTGCTGTACACAAACACCTGATACAGCGAATTGCCGGTGGTGTATGAGTTTACGTAGTGGGTGCCCACATCTTCCATAAATCGCACTGCGGAAGTTATATTTCCGATTTGCAGTTGATCCATTCTCGATCGCACATCCGGCTCCAAGGGTTGGTGCACCGGAATCTCAGCGTTAAGACGCCCCGTTTCCCTGAAGCGCACCACCCTCACCAAAACATACGAGTAATCGCCTTGGATGAAAGATGTATTGATGCCCAGCTTCTTGGCCGCATTATCCGGAAACCATCCGCCAGTAAATGCCTCCCACGGTTTGTCCATTCGCTCAATGGAAAAGTCACGGAAGTAGGCCTGGAACAGTTGCCTCCGATTCTCGCAGAACTCCATGTGAAAGTCTCCATGAAAGATTCCGGGCGTGGTGTCTTCATGACTCTCCGCCAGGCCACTCAGATTCTGAAAGAAAAAAATCATTAATTATCTAATTGTCTAGTCAGCTCAATTATCAGGTAATAAATATCAAGTGACATACTTGAATAATCGTATATAATGATCGTTTTCCTAAATCTGATCAAACAAATATAATAAGCGCTCATTCTAACGACAA

ACGTGGCGGAAGTGAACAATATAATTACAACTAAAAATAGTTTTAAGTGATAACAACTTTAAACCCTATTTTAAAATTTTGATTATACTCCAAATAATTATGGTGATTGTGGTTAAAGGTTAATACAAACAATTCCGAGTTCATCAACGCCATTTAATTTATTAAATGTAAGCTTAACTCTGCCTTAAGTCCCTTACCCTATAGATGTTTTTGGTGGGCTCCTTAAACACCCAGCGATCCGGCTCCGAGTTGTCATTGAGCGGTATCACACGCATGGAGATTCCCAGGTAGCCATAGCGCAGGAATATATTGATGGCCTTGCCGATCCGGAGCTGGGACTCGCGACGACCTCCATCCGCCAGGAGCGCCAGGGTCAGCAGCCAGAAAAGGCCAGGCCACGACCGCATCGCTCAGCATTCAAGGTTAGATCTATAGAGGATTATAATAGAAATATTATATTGCAAATTTAAAGCGATTACACTTGAATTAGGCAAACGTTTCCATATTCCATATCCATATAATCCCTTGCTATAAAATATTATTGATTGAAACAAACGCAACCTATTCCGATCTCTTATAAAATTCGCAATTTAACAATAATGAGGCATATCTATCCCACAATAATTTATTCAAGTGACTAGATAACTCTTGATTGCAATGTGCATATTGATGACAAGCAAGTACAAACAGCGCTATACCATCCGAACTCATTACTCACTTAGACTAAAACCGGAAAGTTTTACAGCAAGCTACTTCGGTGGTTTGTTTATATTTTATAGCCAGTGCAGTAGTTGCGATACAGTGGGGTTAACTAAACAGCAATAATTGCAGGGCCGCCGTGTTTGTTTTCCACTCCATAAACGGGCGAT

 

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