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CRIMIC CR02117

Gene CCHa1

CG14358

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3R:14092504..14096835	88A8	FBti0213458	91440

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGTGGTACAGCAAGTGCAGTTGGACT	AGAGGACACTTGGTGAGGCATGTGAA

Images

Construct Design Sequences

sgRNA sequence	GCTTGACGGGTTCACACGTTCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCCCATAGAGCCGTGCATAT	TGTGAACCCGTCAAGCTGTG	GTTCGGGATGGGTTTGGGT	CCCCACCGAGTTGCATACTT

 

Amplicon 5'

Amplicon 3'

GCCCATAGAGCCGTGCATATGGAGCCCACACTTGTTGACAGCCATGGCGGCCATCAGAGATAGCGATTGCCGACATCGAAGCCTTTTTGGGGTCGCCGCATCGAAGGGCTGTCATTAAACGAGCTATCCCCCCACCCCATTTTAATGGCCCTCTTCAGCGGAATCCCACACCCATTCCATGGCCCTTCCCAGTCAGCAGCAGAGATACCTAATTAACTTGACCAGTGCATTTTAATAGCCATATCCTTGATCTTGGCCAGCGTTGGCGTCTCCACTTCTGAATAATGTCTATTTTTATGACCTAAAAGTGCGACGCAATTTAACAGAAAACCCCATTTAATGGAGAATTTTAGCAGCAGTGGGTTAATATCGGACTGCGGTTATCTAAGTGTCGTCTAACTTTTTTTCAGGGAATCCCCGAGCAGAATAAAGTCATTGCCACCACATTTGGAAGTGTCATTAGAGGTGTGATTTAATGTCTTTAATTGCTTAATAGTGAGCTGATAATTATAAATTTGTCATGACATTCTGAGATATCAACAAAATTTATGCTAGAGAGAAAAGGCATATATATAAATTAAATATATATGCACGACTTCTATACGAACGTATTAATTATTTGCCTTATCAGCATTTCGGTTACTGATTGAAGGGCAGATCAGATGTTATGAATTGAAACCATGAAAACCCAAATAGTAATTTGTCAAGATAAACTTGTCGTCTACACTCACAAAATAATTTGGCAAATTTCCCCATTGCTAATCGACCTGAACCTAACCCAATTCGGATTTTTCCGCGGCACGAAGGCTGTTCTTTTGTCCATGGCCAATCTGCATGGGAATCGCGTAGTGAGGGGTGGTATATATGTATATGTATCTATCTATCTCACTGGAGGCGAAACCCACAATTGCAATGCCTGCAGGCGGCACAGCTTGACGGGTTCACA

GTTCGGGATGGGTTTGGGTTTTTATTTTTCCATTCTTGCTGACGCTCTTTACGCAACTCCGTGGGTTTCCAAACTACGTGAATTTCCATTACCACCGTGTCACGGATGCACTCAGAAAAATATGTGATGCAATTATTCGTTTCTATTTCAGCTAAAAACTTTATAAACTTTATAAACTATTTTTCTTATATATTCAAACTTTTAGCTAAAGTAGGGAATTTGTAAAAATCTTTTAAACTTTAACCATTAAGATCTTGCTAAATATTTTTTATCCGTGTGATGGTCTTTGGCACGCAGTTGGGATTAATTTACGATGGCAATCGAAAGCGTTAAAAGCGTGTTGGCAGGGCAGTGTGTCGGTTCCGATGGTGAAATCGAGAGCCCCTGATCTCTCCAATCCGCAGTGGAAAGCCCTATTTCCCCCGATCCCCGGATTCCACCTGCTAGTCTGCATTCGCAGTGAGTAAGGAAAGTACGTTGAAAAACCCCAAAAAAAAAAGGGAAGCCGCCGGGCAGGCGAACAAACAAAGGCGTATCAACAGGAAAATGTGCATAAAACTCCAACTGATAAAGCGGATTAGAGGCGTAAATGGAAGAGGAGAGGGTGAGATCGGAAAATGGGAGCTGGAGTTTACCCGGATGACCATGATATTCATAACAAGTATTACCAGTTAGTTAATCAGCTAGTAGTTTGGTATTTTTATGGTCTAGATAGTCACCCGAATTCTTCGAACCAGGTGAGTAAGCATAACAATTAATTTCATCAGCACCTCCGCCCGAAAGTATGCAACTCGGTGGGG

 

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