CR02120
CRIMIC CR02120
CG11000
Information
PCR Verification
PCR check relative to sgRNA sequence
HA-L |
HA-R |
Forward |
Reverse |
OK |
OK |
TGGTTGATGCAGTTCGTCGCATGCAT
|
AGCGCAGTAGATAGCCTCCCTTGATT
|
Images
Construct Design Sequences
sgRNA sequence |
CAAACTGATTACGACCCGAGTGG |
sgRNA2 sequence |
|
Amplicon PCR 5' Left |
Amplicon PCR 5' Right |
Amplicon PCR 3' Left |
Amplicon PCR 3' Right |
AGCCAGGCCAGCAATTATGT |
ATTTGCTCCGCCTGGGAATG |
GGGTCGTAATCAGTTTGCTGC |
GAGGCCAGTCACACGATACC |
Amplicon 5' |
Amplicon 3' |
AGCCAGGCCAGCAATTATGTCTGTACACGTTTGTCGCTGTCTTCGCCTTCAGATTCGTCCTGGCCCACTGCGAACGGGAATCCGAGTGTGAAGTGGGCCAGGTTTCGTTGTCTGTGGCAGGCACTTGCAATTCTAGCTGATTTGTCATGTGGATGTGGATGTAGTTGTGAATGTCGATGTGTGTGTGGATGCAAGGATGCGAGCATGCAGAATGCCCACATGCAAGTGCGGCTGTGTGTGCGCGTGGGCATGGCTACGTGCGGTTGTGTGCGTGCAGTGAAGTGGCACACATGATGTCCGCTGCCCTGCCTTCTTACACAAGCAAGGCAGTGTCCTTCGGCGAGCTCGTTCTCACCCCAGCAACCCCAACCCATGCAACAAACATGCCAAGTCCCTTGCCCCTGGCAGCCCCAGTTCCCAGCTCATCCTTTCATCATCTCCTTCGACACTTGGACTGCGTCTATCGTAGTCGTCTGCAGTTCGCTTGCTTCTGCTACACTTGCAACATTGAAGAAAGTCAAAAGACTGTCAACTATATATGTTTTTAAGTAATATACTCATAACTCGTGGAGTAAATAATAAACTTAAATCATTGAAAAGTATTTTGAAGGTAGAATGAAGCCCCCTTTTAGCCAGCAAATTGCTAAATCCTGATTTCTTTCAGGACCTCCTTTAGCTGAATAACAGGTATCCGATTGCTTCTGCAGCTCAACTACACCATTCTCTCTCTTTTATCATTGACCAAGACCACTAGGCCACATATATTTCTCAATGTGTATGAAAATGGCAGAGGTCGCAGGGAAAAGAAGGGCAGATGCAGACCATTTTGAGGGCAAGCGCCGTTTTTTAGCACGTCGAGCCTCGAGCGTTTTTCAAATGCTGTGCGGCCTGTGCAGGCCAATCGCATTCCATTCCCAGGCGGAGCAAAT |
GGGTCGTAATCAGTTTGCTGCAAAGGCAACTTTTCGAATGGTCGAGTGCGGAAAAAATGCCAGAGAGGTCCTTCAATTCCGAAATTGCAAGAATATCACCCGAGGCACATTTAATCGCCTATCAGATTCTGGGAACAAGTTCAATGCTGGGAATTTATGAGTTATTGCTCTTATGAAATCATAAAAAGTTATAAAGTTATTCTATATTTTTGCTGATTTCTTCAAAAATCTTTATATTGTTTATATATTTTTATTGTAATAAAAAAAACCCTTTAAGGGTAAACATAATCCTACCTAATATAACTTGTTGGTGATTTGAAACTGATGGTTAAATATTACTGGCACTAGTATGGGATTTCCTACTTTTTTGAATGAACCCTTTTCCACACATATTGAGTTTAAAGGCAAAAGATATAAAGATATTCGTTCTTACTGTTACACAGTTGAGCAATATGAACAAATTAAGGAAATTAAATTTAAATAGAATTCTACACTTTATGTTGAACTCGTCGCTGGTGGCAAAGGTGTGAAATCGCTTCGGATCGGTTGCCTTTGCCATCTTTATTGAAATACTTAATTCAGTTCACCGCAAGCGTTGTGTTTGTCACTACCCCAAATCCTCCCACTCCTCCGCCTTCTCTGCCTTCTGGCGCCCACCTGCGACTCTCGATACTTGACCAAATAAATGGGATGCCAGCGGTAATGAATTTGAAATGATCGACCTGGTCCAGCCAGCAGGAGCGGCGGCTGGCTGGCTGATTGAGTTGCTGGCGATGCAGTTCCGGGGGAATTGCTAAATGAAATTCCGTGTTTCGTTTTTCGCCATTCGTTATCCGGCGCAGCAGTCGCACATGGACTCGCCCACGCTTGAAATTTTCAGCTCCTGCTTCGGTGCGTGGGCGCTTGGTCCACGACTGTCGGCTGTCGGATGTCGAATGGCGACGAAAAGGAAAGAAGTCCTTGAATGGAAAATGGGCTCCAGCTGCAGACCTCGATTCCTGTTCAATTAGTGGTATCGTGTGACTGGCCTC |
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