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CRIMIC CR02120

Gene CG11000

CG11000

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3R:5802328..5813258	83C4	FBti0213460	91441

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGGTTGATGCAGTTCGTCGCATGCAT	AGCGCAGTAGATAGCCTCCCTTGATT

Images

Construct Design Sequences

sgRNA sequence	CAAACTGATTACGACCCGAGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGCCAGGCCAGCAATTATGT	ATTTGCTCCGCCTGGGAATG	GGGTCGTAATCAGTTTGCTGC	GAGGCCAGTCACACGATACC

 

Amplicon 5'

Amplicon 3'

AGCCAGGCCAGCAATTATGTCTGTACACGTTTGTCGCTGTCTTCGCCTTCAGATTCGTCCTGGCCCACTGCGAACGGGAATCCGAGTGTGAAGTGGGCCAGGTTTCGTTGTCTGTGGCAGGCACTTGCAATTCTAGCTGATTTGTCATGTGGATGTGGATGTAGTTGTGAATGTCGATGTGTGTGTGGATGCAAGGATGCGAGCATGCAGAATGCCCACATGCAAGTGCGGCTGTGTGTGCGCGTGGGCATGGCTACGTGCGGTTGTGTGCGTGCAGTGAAGTGGCACACATGATGTCCGCTGCCCTGCCTTCTTACACAAGCAAGGCAGTGTCCTTCGGCGAGCTCGTTCTCACCCCAGCAACCCCAACCCATGCAACAAACATGCCAAGTCCCTTGCCCCTGGCAGCCCCAGTTCCCAGCTCATCCTTTCATCATCTCCTTCGACACTTGGACTGCGTCTATCGTAGTCGTCTGCAGTTCGCTTGCTTCTGCTACACTTGCAACATTGAAGAAAGTCAAAAGACTGTCAACTATATATGTTTTTAAGTAATATACTCATAACTCGTGGAGTAAATAATAAACTTAAATCATTGAAAAGTATTTTGAAGGTAGAATGAAGCCCCCTTTTAGCCAGCAAATTGCTAAATCCTGATTTCTTTCAGGACCTCCTTTAGCTGAATAACAGGTATCCGATTGCTTCTGCAGCTCAACTACACCATTCTCTCTCTTTTATCATTGACCAAGACCACTAGGCCACATATATTTCTCAATGTGTATGAAAATGGCAGAGGTCGCAGGGAAAAGAAGGGCAGATGCAGACCATTTTGAGGGCAAGCGCCGTTTTTTAGCACGTCGAGCCTCGAGCGTTTTTCAAATGCTGTGCGGCCTGTGCAGGCCAATCGCATTCCATTCCCAGGCGGAGCAAAT

GGGTCGTAATCAGTTTGCTGCAAAGGCAACTTTTCGAATGGTCGAGTGCGGAAAAAATGCCAGAGAGGTCCTTCAATTCCGAAATTGCAAGAATATCACCCGAGGCACATTTAATCGCCTATCAGATTCTGGGAACAAGTTCAATGCTGGGAATTTATGAGTTATTGCTCTTATGAAATCATAAAAAGTTATAAAGTTATTCTATATTTTTGCTGATTTCTTCAAAAATCTTTATATTGTTTATATATTTTTATTGTAATAAAAAAAACCCTTTAAGGGTAAACATAATCCTACCTAATATAACTTGTTGGTGATTTGAAACTGATGGTTAAATATTACTGGCACTAGTATGGGATTTCCTACTTTTTTGAATGAACCCTTTTCCACACATATTGAGTTTAAAGGCAAAAGATATAAAGATATTCGTTCTTACTGTTACACAGTTGAGCAATATGAACAAATTAAGGAAATTAAATTTAAATAGAATTCTACACTTTATGTTGAACTCGTCGCTGGTGGCAAAGGTGTGAAATCGCTTCGGATCGGTTGCCTTTGCCATCTTTATTGAAATACTTAATTCAGTTCACCGCAAGCGTTGTGTTTGTCACTACCCCAAATCCTCCCACTCCTCCGCCTTCTCTGCCTTCTGGCGCCCACCTGCGACTCTCGATACTTGACCAAATAAATGGGATGCCAGCGGTAATGAATTTGAAATGATCGACCTGGTCCAGCCAGCAGGAGCGGCGGCTGGCTGGCTGATTGAGTTGCTGGCGATGCAGTTCCGGGGGAATTGCTAAATGAAATTCCGTGTTTCGTTTTTCGCCATTCGTTATCCGGCGCAGCAGTCGCACATGGACTCGCCCACGCTTGAAATTTTCAGCTCCTGCTTCGGTGCGTGGGCGCTTGGTCCACGACTGTCGGCTGTCGGATGTCGAATGGCGACGAAAAGGAAAGAAGTCCTTGAATGGAAAATGGGCTCCAGCTGCAGACCTCGATTCCTGTTCAATTAGTGGTATCGTGTGACTGGCCTC

 

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