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CRIMIC CR02124

Gene CG13618

CG13618

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3R:24524794..24527533	96A5-96A6	FBti0213461	91442

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	long band	AGAGATTGATGGGATGAAGCAGAGCG	ATCCACCAGGCATTTGTCGAAGTTCG

Images

Construct Design Sequences

sgRNA sequence	ATCTCTGTAAGATTCTCTAGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GTCCGGCTTAAGTGGTTCGA	TTCTGGTCTGCAGGCCAA	TGGCGTTTATAGTTGCATGCA	AGGCACACATGACCTATGCC

 

Amplicon 5'

Amplicon 3'

GTCCGGCTTAAGTGGTTCGATTTGACTTCCTCGAAGAGAGCGAAGCGTGAATATCGTATAAGCCATATATACCCCATATTATGGCCATAAATTATGTTGACAACCAGGTAAGGTTCAATGAACCGCATAGCGGGGGAAAATTAAATCGAAAGGGCAAACAACAGCAACGAACAGGGTTGCTGGGTGGCTCGGTCATTGGTCGTTGTCGTCACTTGTCTTCCACTTCCAGTGGTCATTGGTGGTTTGGCTATCTTGGCCTGGCCAAAAGCGGCGAGCCGGAAAGCAAAAGTGGTTCGCTCACTGGCTGGCTTAATGGGCCAAAGCTCGGCGCGATTGAAAATCGATGGGTTTTCGAGACTCGCAAAAGCTCTCCGACTTCCAACGTTCAGAAAGCCAACTTGAGGCGCCGTCGCCGCCGCTGAAGCTCGGTATAAAAAGACGATCGGCGGACGCATCCCGTGTACACAGTGATAAGTCTTCGGCCAGAACCACCTGTCACCTGAACCGAAAACGGAAGTCGCGCGCGCGTGTAGCCATCTCTTCGTGCCGAGTGTCAATTGAAATTTGGCTGTGGCTCTTGGTCAATCGATTTCGTACATCCGATCCGATTTCGTACATCCCCCCGGACGAAATATGTCAAAATACTCGCTACTACTAATGCTGCTGCTAATCGCTGCGGTTGCGCAGGAACTTACTGTAACGGCTGCGCAAGAATTGCGTAAGTACTGCACCCAAAAAAAAAAGAAAAAAATACAAATAAAAAACAAATAAATTAAGCAAATCGAACATCAACTATTGCGGCGAGTCAAAAGTGCAAAAAACTTATAGATCGTATTTGCTTTGGCGCTATGCTTCGGCTACATCTATTTGCTTGGCACCACATTTATGGTTAAAACGTGATCGATATGGCTTATGGCTCTTTAATTTCGTTTTTTTTCATTTGGGAACTCGCGTTTTGGCCTGCAGACCAGAA

TGGCGTTTATAGTTGCATGCAAATTTGGAGTGTTATTACAGCAAGTACAGTAAAAATCAGTAGAGTCACGCCGGCTTAGACTTAAGTACTGCTTCAAATTGGGATTCTGGGATTTACCTTATTTATATACTGGATTTTTTTTACATTCTATACTTTTTAAAATATGCATGGTCAATTTCAGTTTAAGAAAGCCTGACAATTTCCCAGAACTGGAACTAATATTTATGTTAACGTTGCTGTAGATCCATAGAAATTGTATTTTTAAGTGTTTTCATGTACATTTTAAATACTTTAGAATCATGAATAATTACTTTTCTTGGTTTCAACATTAATCAAAGCTAGATTTAACTTTGAATCCCAAAAGAAATCCAGCAAGACAAATATGAACAATTAAAAACAATTAGCGTGGGAATCACCGAAAAGCTAAATAAAATTATTTTCCATGAAACGATTAAAAATGCGATGGGCTATTTCCAAGATAATAGTTTTGGCAATTAAAAACGAGAATAATTGAGGGGAGATATAAAAAAAACAAGCGACCATAAGACTTTATGCGCTTCATTTGCGTGCTGCTAAATGGTCAAGATCAATTAAGATCGGTTTTTTTTGGCAGCATTAGAGGTTTCCAGCTACTGAAGTCCACTGTAAACGGCGGTTATGCAATTGAAGCCATCGTCTGCAAACAGGTTGCTCTTGGCCAGGCCAGTTTGGCGGTCCAGACACCACAGGCCACAGTCCAGTGATCCACATTCACACTTACACTTCCGCTTGTGACACAAATTTACGATTATCGCAGAGAAGGACATACATATGTACATATGTAACCGAATGGCGATTAAATTGTGTTAATTAATTCGCACATTCCACGCCGCAGCACGGAATTTTTATGGAATTTTTCTCGCTGTGTAGCTGATTAATGAAACCGTTGGCATAGGTCATGTGTGCCT

 

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