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CRIMIC CR02125

Gene CG15594

CG15594

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3R:6287733..6289824	83E2	FBti0213462	91443

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTGGAACACTCACCTCCTCACAGTTC	GCAGTCAGCAGTCGACATTCGTTAAC

Images

Construct Design Sequences

sgRNA sequence	TCACAGCCGCCCCGAGGTTATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTCCCATCGATCCATCCAGC	GGTCGCTTGTCACTTGCATG	CGGCTGTGACTGACCATCTA	TGGGAATTTGGAAACCCCGT

 

Amplicon 5'

Amplicon 3'

CTCCCATCGATCCATCCAGCTATGTTCTGGCCATCAGCCAGCAGATGCGGCGGGGCCAACTCCTTCGCCAGCTGGCAAAAGCGGAGGATGCCGAGCCGGAAGTTCACAAGGTGGCTGCGACTTTAACCCACCCGGTTAATGGTGATGACGATAATAATCAAGATGATGAAAACAGAATCCTTCCCGGGGGACCAGAGGATAGCGAACAGGGGTCACTTCGGGGTGTTGAATCCTACTTGGCGCCCTTTGAACGAGCCCTGGTGAAAGTGCGCAACTTCTGCGACGCCCTGAGAAGCGTGATTAGCGACGAGGCAGGTGGGTAAATTTAATGAAAACTTTATTAAATGTGGTCTAAACCCAGGAACAGTACATGAATTAAAGCGAATTTTCATTCCAAAAGTGTAGTGCGCAACAACATGAATATTAACTAAACCAAACTAAAATTGTTTTCCTAACGACTTCAAATAGTAAATAAACAGCTACCGAAACCTTAAAGGAATCTTGCACTTCCAAATTTATTCCTGGCTTTTCAGATGAGTTTCCAATTTTAAAAACTATTTTAAAAATTTCACTATTTTGAAAAAAGGGGGGTATAATATAGCCCTATCTGAAGGTTTTCAGCTTCATAAATATTCCAGAACAATAGTTGTATGGTTAGAAAAAACTGGACAAGCAACTGCGTTTAGCTTCAATAACCATATACAATATTTTAGAAAGTATGCACTAAAATATTTCAATATTATACAACTTAACGGGTTTTAAGTGCATTACTCATTATACTTGCTATACTTATTAATTGACTAAGTATAGGAGTTTTAAATTCAATGATTCTTGTTTATTTTCCAATCCAACCCATGCGGTCATGCAAGTGACAAGCGACC

CGGCTGTGACTGACCATCTAATTACGTGGCTAACTATTGTTTCCGCTCACCCAATCGCAGATGAGACGGGCACAAATAAATTGGATGCCATCGCAAGAGAAGGAGCAGCAGCAGCAGCCGCAGACCCACCAGGTGCAACTTCAAAGGAGCTCCGGGTTCAAGGTCGCTTTCAACGCCTGGCATCTGCGGAATCCGAGGGCCGCAAGCTCAAGAAACTGAAGAAGAAGATCCAGAAGCTGTTTTTCCCCCTACTGATTGCCTACAAGCTCAAGTTCCTCACCCTCATCCCGGTCCTAATCGGCGGACTCACGCTGCTCGTGGGCACCACGGGCCTGGCCGGCTTTTTCTTCGCTCTCTTTACGGCTGTAATGAGCCTGAAGAGCTCCGGAGGTGGTGGCCACTCCAGCAAGTCCCTCGTCTTGAAGAAGCTCTGATGAGTGGCGCCTTAAAACCCAATCAGAATACCTATTTGCCTAGAGTTGGCCCACTGCTCGGCCAGCATTGATTTATCCGGTCGGTCGGTGGCTATAAACAAGTGACGTCGCCGACTTTCAGCGATAACAACGACGCGCCCGCAAATGAAATGAAGTATGTAAATAACAGCATTGTCATCCTAAAGCGAATTTTAAATACTCTGTCTTAAATACTGGTACCTTAGACGCAATCTTTAATATATATATTAACTATTAGCAGCTCCATGAGAAATTCGAGCTTTACGAAAACTAAAATTTCTTTTTTAGCCTTCTAATTTTTGGATACATTTTTCTTACGTTAAACATAACACGGTTTGGTTTAAGGTTTTTCTCTTCATAAAGTCAAATTATTAAATATTTTGCATAGATGGGCTCTTAATTATAAGTATATATTTATCTTGAAAAAATGATCTTGAAAAAAAACGTCTTTAATTATTTTTTACTAAGCAATGAGAACTATATTCTTCCTAAATTTTTACTATTTCGATTCACTCGCCTATTTATAAATTAATATGAAAAAATTGTTATAAGATTATATATTTCTAAGTTATTTTTTATAATATTCAGGACGTGCTTACCGTTAAATTATTATCATTTAAGTTCAGTGCAAAGTCTAAAAATAAATTTCAAATTAATTGTATGTGTACCCATGCTATTAAACATCAGGTTCATTCCATCTAGAACCCTTAAAGAAAGACGCATTAAACGGGGTTTCCAAATTCCCA

 

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