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CRIMIC CR02126

Gene CG17111

CG17111

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3R:23062713..23067259	94D10-94D12	FBti0213463	91444

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGTGTACTGCTTCTGCTGCGTCACTT	AGTCCTAACCTATCCCATCACCATCC

Images

Construct Design Sequences

sgRNA sequence	TTAACTAAGCAACTAACTTCTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGATTAGAGGCGGATCACGG	GAAAAGCGGAAGTGGAGAGA	GAGCACTTTCCTGTGTAGGTCA	CACTCGATGATCTCTCCGCC

 

Amplicon 5'

Amplicon 3'

GGATTAGAGGCGGATCACGGCCACTTCCGGAGGTTTGGCTGGAGAATTACTCACCTCGGAGGCAGGTCTTTTCGTAGAAGACGGCCTCGCCGTCTGCCACCAGAGGCAGTTCACCCGAAATGGCCAGATAGCGCGAGGTTCGCTCGAAGTACGAGTAGCCATGGCACTCGTCCGTGTCCAGCAGATGGACATAGGCAATGCAATCGGGATCCTCGTTGCAGATGCGCCAGCACACCTGGCTGACCGGAAGTTCCTGTTGGCCGTTGCGAGTCTTGAAGTTGCTCACCATTTCCGGTCCGTAGATCCGATTATCCGCATTCAGATACTCCAGCGGTGGCCGGAATCCGACGATCTTCTGCAGCTGCAACTGGTGCAACGGACACTTGAGCAGCAGCGCAACTGGGGGGCAGAACGGAATGCCATGAATGTGGGTTTCAATCCAACGGGATGCGCATGACTGGGAACCAACGCATTTTTCAAATTGCCGAGCAGTCATATGGATTTCAATTCTGGTAGCTAAGCTCGTGCTCATCAATTAATATGATATTTTTATGCATAGCTGAGCAATGAGAAAATCAATCAAGCATCATCGGGATTTACCCTTAAGTAGGGAGGGGGTTTAGGAATGTCTGAGATTGGCGTTATTAACTAATTGAAAGGTTGAATTGGAAATTAAAAAACGTAACTTAACAAACTTATCATGAATTGCAATTGAGATATGCAGAGATCTTTCAAGGTATCTGGTAGATACTTTTTTGATATTCTGGCATATCTATAAGATAGTACTTAATCTCTCCACTTCCGCTTTTC

GAGCACTTTCCTGTGTAGGTCATTCTCCCACTTAGGCCCCCCAATGAAACTCGTTCTCAGGGTGTTTGCCAATAAATAAATCTATAATAGCAGCTGACATGCACATGCAGCGAGTAAACCCCTTTGCCGACTTGTAAATTGATCTATGCAGTTTTGGTTTTGCTTCTCCATTTATTCGCTTGGCTTTTGAATGCAAATGGCGAGTGCATCTTCAAAACGCTCTCGGAATTGGCATCATCATGAGCTAAGCGCGGAGCAATGTTTGCAAAGGGGTTTTCCGATTTTTCATATTTCATATATATACATATATATATATTAGCCAACTGAGAACTCACCACTAGCGTTTAGCTGGAGAATTAGCAAAATCGCTGCAATGGCTAATTTGCATAGCCGGGGGGCGGTCACAAGTGATTGGAGTGGTGGCATTGTTTTTCAGATGCGTTTTTGTGGCTGCAACCGCGAATTGTTTGGCATGCTCGTGCAATAAATTTTAATTGATTCTCGGTCAGAACACGGGCTAAACTCGTAGACGCATTACACGAACCGAGTAACAAGGGCCATTTATTCAGTGGCTTTTTGCCCGTTAGCTGGTTATGTATTTGGCACAGTCCAACACTTTCGGTCACTCAATCGCCTGCAGATCGAACTATTTGCATATATGCAAATGCAAATGAGCCAGCAGTATCCTGTTATCCTGTTATCCTGGCCCACTCGACAACAAATATGAATTAGAGCGATTTCCCGATGTGGGTCAGTTGGCCGCCAGCATCATCGTTGTGCTAATTACTTCAAACTCGCCTGCTTATGCATTATTTATTTGTTTTTAAATTCGAGTATAACGTGTGGCTTTGACTTTTGCCACCACTTCCATTAGCCAAATGCACTTGGCTAAAGTTCTGTTCACTTTTATCACACTTGATTCCGCTGCGGCGATTGTACGTCCGACTCGCGCGACTCTCGACTTGCAAATAGCAGACGATGATCTGGGCTCCCAGGCGGAGAGATCATCGAGTG

 

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