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CRIMIC CR02127

Gene CG31064

CG31064

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3R:27229505..27239085	97E10	FBti0213464	91445

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
long band (weak)	OK	TTGCCAAGCACGCTCCTACTTTGTAG	CACCAATCTGTTTGGCAACTACCGCA

Images

Construct Design Sequences

sgRNA sequence	ACTCGGCGGTACTACTACTCCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CAGTGCAAAACGACGCTCAA	AGTAGTACCGCCGAGTAGCA	GTCGCTCCACCTCACAAAGA	AAAAGTCGTTTCCCAGCTGG

 

Amplicon 5'

Amplicon 3'

CAGTGCAAAACGACGCTCAATACGAACGGAAATGGGTGGCGAGCTCCGGACAAGGATATATTGTCGCAGTTCAAGGTACGAAAGATCGAACCCGTCAAGCTGCCCAAGAGCTGCTATTCCCAGGAGGATGCCTCCAGCTGTGCTCAACTGGATCAAACATGCATTTCCCAAGAGCCGGATCAAGGGAGCATGATTGTGAGCCTGCCTCGACTGGAGACGCTCGATGAGAATGGCTATATGGGCAACATATACTTGCCCTTTCGAAAGCGACGCATCTACAATCTGCGTTCTCCCAAATCGGAATTTGTCGTGGTGAAGTTCTTCCAAACCCTAACCAACTGTCATCGGTTTCAGGGCATTAGCCAGGCTAATTACAATCGCCAGTTGCTCGCCTGGATAAAGGAAAATCTGGAAATGCACTATGGCGATGATGTATTCTACCCGGGTCTGGGCGGTATTCTGCAGATTCAGGAGAGGCTGTCGAAAGAAGCGAAGAATGCGGAGGAGGAGGCCCGGGCGCCAACCAATAAGAATGACGGGCAGGGATCACCATCCTGTCCCAAAGAGGACTCCCGCAAGATGAGTCGCTCCGCTATTCACTGGCAGGAGGCTTCAGGGCCAGTCGAGGAGTCGGGGCCGGAGCCAATGCACCAGGACGAATGGCTTACCGGATTGGATAAGGTTCAGAGTCGGCCGGAGGAAGTGGAAGAGTGCCGCGAGTGCGACAACGATGAGACACTGATCTGGTGCATTGCCGCCTTTCTGACCCTGCTTCTGCTCCTCATTCTGCTCATCATTTGCTGTTGCATGCGAAGGGGCAGGAAGAAGAGGGTTGCCCCAACGGATGCAGAGGCTCCTCCGCCGAAAGAGAGGCCAAGAAAAGAAAAGGAGCCGCTGAAAGCCAAAGAACCAGAGAAGGAAAAAGAAAAAAAAGATGAAAAGGACGAAAGTGATGCTACTCGGCGGTACTACT

GTCGCTCCACCTCACAAAGATCGGTGCCGAGCAATCGTAAGTCAAATAAGTTTGTGGCAACTAAATCAACTAAATCTAACTGCTCGAGTTTATAGGATATGCGAATAATCCCGAGCTAGATTCCTTCACCTCATCTTCGTCGCTGGGATGTCAGTTCAACAGGAAGTGCATGCCACTGAAGTTCTCCCGAGCGAAAGGAGGGGACTACTACGCACCGGACAAGAGCCGAGATGCGGTGGGAAAGCAGGTCACATCCCTCAAACTTCTTTCCGATGATTCGTCTCCTAGCCGAGTTCCCTACAGGGACGTTTGTAAGCCCGCTCTAAAGTCGACCCTATCCGTCATTCCCTCGTCGGACAGCGAAGTGGACAGCAGAGAGGATAGACATCGACATAGGCATGCCCGCGAAGAAGACAGGCGGAAGAAGCATAAGGACTTTGAGACGGGTCGTAAGAAAGAGGTAAGTTATCAGTGCTTCGCGTGCTTTTGATATCCTGAACTCAGAGTCACCAGCAGACCAAATCCTCAGAAGACAAAAAGCAGCCATCACCAGAAAGGCGATTGTCCAAACAAGTGAGGATAGGTAAGTGCATGACTGGTCATTTCTTAAGCCTAATCGATTCATTCACTTAATCCTCTGATGCTGCCATCTTAAAAGCAACACCGGAAATGTACAAGGATACATCCGATGATCCTCTTCTGCACAGGAAGAAGTCGCAGGAGAAACCAGATGTCCCTGCCGGTCGACAGAAGGGCGGTGCAGAGGTGAGTGGGATGTGCAAAATGTTATATGCCAAAATAATGATCTTGACGGTTTTTCAGTATTTAAAGGACTCTCCCAGCTGGGAAACGACTTTT

 

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