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CRIMIC CR02128

Gene CG31321

CG31321

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3R:14310362..14314412	88B3	FBti0213465	91446

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	long band	AGGCATGTGCGAATTCCACAGTTCAC	TCGCAATCAGCTGAAAGACAATGGCG

Images

Construct Design Sequences

sgRNA sequence	TAGGCTGAGGTCTTTGGAAGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTCATGGCCTCCGTGTTCAA	AATCACCCGGCAATAGCGAT	CCAAAGACCTCAGCCTAGTGG	GGCGTGGCTATGGTGATGTA

 

Amplicon 5'

Amplicon 3'

TTCATGGCCTCCGTGTTCAATGCGGTGGCCATGCAGAATTTCCCACTGGACAAGGCGTGTCGCGTGAATCTCGGCTACAACAAGATCGTCTGCGACACAATGCTGGACAAATCGGAGCTGGGCATCGAGTGCGATGACTTTGATTTCGAGAACACCACACAGGGCGCGACGCCGGATCTCGCTGACCTTGTCATCGGAGCCACTGGCTTCAACTACACGGTGTGCAAGGCGGAACTGGAGGCACAGATCCTGGCGGCAGATGTCTCCGGGAAACGAGCTCCCATGGGTAAGCCAGGCGACAAACATACACATATATCATACACATAACACGAGTCGTTCATTAAGTATACGCCGTTTTGATTTCGGTTATGGGTTTGGCATTAGCATTGCTTATATTTCAGGTTTGTGCTTAAATTAAATTCGAATTCTTTTCACATCTCGACTCCTCTGCATTTTAAATTTCCCCTGCCCCTCGAATGGGTTAGGTACACTCGAAAAATAATAGTATTATCAAGTGATAATGGGACTAGAAAGACATTGAGATACTTTACTCAAGCATAAGATTGTAAATATCTTAGAGAACTTTCTTTAAATTGAAATGGAATAGCTATATATACACATTTTCTTTGAGTGCACATTTTTTTCTGAAGGAATCCGTGTCTTTTGATGGATGTCGTCGGAAATTAGCGGTTATCCGAACATCTAATTACCTTCTTTAATCGATTCGCTCATAAGTTTCTCCTTCGCCTTCTGTCTTCTGATGGTGCTAACAAAAATTCCCCCATTCTATCGCTATTGCCGGGTGATT

CCAAAGACCTCAGCCTAGTGGATACCTAGTAATCGTAATCGCAATCGCTCCAAACCTGTTCCTTACATCGTTGTGCGCTGAGTGACTTTCGTGGGCTCATAGAGCCATTGTCAATGGCACTTTGTTTGAGCCATAGATAATAGCTTATATTCCCAATTTTTAGAATAAACGCCATCCGAATATGTCATTTGCATAGAGCTGCGCGGAATTCCGGTTCTGTGCGCCAGAGGAGATCTTCTTCTTGTTTAATAACTCAAACAAACGGCGGGTTCATTAAGATGCATTGCATCAGAGATCGATAATTGAGGCTTTAAATGATTAAATCGTCATGTCATAGTCTCAATGGTGAAAAAGTTTAGTATACATACATACGTATCAAAGGCATTCGTTGTTTTAGAATGCTTTAATTCCACGGAGATAACTAGTTTCAATAAAATTCAAGTCAGAAATTTAAATCATCTTCAATACTCGATGCCATTTTGGAATTTGGCAAAAAAACAATAGGGTTTTAAAGCGGATTTATAATAGTCTTGAGCAATAAACTCACTAAAATCTCATAGTAAAAATAAGTAAGTACTTCTAATTAAAATTTGCTGTTAGCAAACTTTTTATCTTGGCAATGAAAACCTATCATATTAACAGTAGAATATAGTGAGTTAAATTGATAAGCGACAATGTTACTAAACAGCTTATTTTCAAGAACGAGTTATTTTGGTACCAGTTCTTAATGTCAAGCAAAATTAGCATTACTAAGCAAAATAATGTTAAATCATTTTATTTTTATTCCAGCTGCCATTTTCCCGCTGATTGTCTTACTTTTTGCTGGAGGATGGGCAGATCGATACAACAAACGAAAGCCTTGCATGATAATGCCAATTATTGGAGAGGCATTATCTTTCACCTGTAAGTAGCTTAATTATCAAAAAAAAAATAATATAATTTTAAAACACTTAAATCTTAGGTCAGATTATCTCATCGATTTTTTTTGAATCGCTGCCAATGGAGTTTGGCGCCTACTGTGAGGCTATAGTTCCAGCTCTCTTCGGAGGATTAACCTTCTGTTTGATGGCAATCTATAGCTACATCACCATAGCCACGCC

 

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