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CRIMIC CR02129

Gene CG31371

CG31371

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3R:30501124..30503506	99F6	FBti0213466	91447

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CACATCCTGGAGGCAACTCTCAACAT	TCATGGTTCTACTCACCTCTCGAGCT

Images

Construct Design Sequences

sgRNA sequence	CATTCTCACAGTCATTTCTTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CAAGAGGAGCGTACGGGTTT	TACAGGGTATCGCATGGTCG	TGACTGTGAGAATGTGCAGT	CAACAGGAAGCCGCTTCAAC

 

Amplicon 5'

Amplicon 3'

CAAGAGGAGCGTACGGGTTTCTTTTCGGCCCGGTGTGTTTTATGGCTTGTTTTGCGCTGATTTACTGATGCTTATCGGCCTGGCGAGCTCGTTTCGAATTCTTAATTCTGAATTCGATTCGCATTCGAATCGGATTCGAATCGGGCATTGTCTTTAGCTAGAGTTAGACCAGCCAGGCATATTAATACTGGCTAATTGGCAGTTAGCTGTTTGATATTTAAGACTGCAGTTCGACTATTTAGCAGCTCCGGCCAGCGCTGATTAACATTTCGCATCCGACTGTTGCGACAGTTCATATTGGCTTGCGGTCTATTGTTTATGAATTGTTATGGATGATAATTCCCCCGCAGGCTGCCAATTTGGAGCAATGATTTGTTAATGAGCCGACCATCATAACTCACACTGTTGTGATCCGCAATCTTTGCTGATTTACGGACTTCAGAGGCGGCGGCCAGCACACTTGTTTTATGGCCCAGTGTTTGAGTAGCGGGCTTCCTCTTAACGACAAACAAACAAACAAACAATTATGGATGGCAGCCTTTGTTTGCCCAGCCCATTCACACCGTCGGATGGGCACTCCCAATGGGAAATAACGCAATAAAAGGCTGATTTTTAATCAAGTGCCAAGGTCAACTTTATTTGATTCCCCAGCTGGCAATTAATGCACATGTCGCGGGGATCGGGAAACCATGAAAACCCATCAGGCCTCGCTGCCCACGTCAATTGAGTCCTGTCCACGCGGCTGAATGCGAAATTGAGATGAATAAAAATGGAAATTAGCAAAGGGAAAGTGAATGTCGACCATGCGATACCCTGTA

TGACTGTGAGAATGTGCAGTTGTTGGAAAGAGTAAGCCATGTAAAGAATTGATATTACTTTTAACGACGATAAAAATATTTTTGTACGAATATTTTGTGGAGAACTTATATACCCCATACAGGGTATCTCGCAGCACGTTCGACTGACAAGCGATGACAGCTTGTTTTGGGATGTGGTAAGTGCGACTGGGTCGCAAACTGAATCCCGGCACGCAGGCGAAATCCAACCACGCCGCAACTTACACAACTGGACGCCCAGGAGCACGGGACAGATGGCCTGGCCACTGCGGTAGTCCCACTCGGATGACGAGCCCCCGGCGAATCGTGTGCGCCAGTGGAGCAGCGAGCCACGTGGGACATTCACGGCGAGCTCTAGCTGCGGAAACACCACTGCTCCTCCCATTCCGGGTGCCTGCAACTGAGCCGCGGGGATCGGAAATTAATTAGTGACACATTAGTCATTTAATTAGGCCGCGCTGGATAACACGTACGTTCAACATGAGACGTGCCACATGCTTCAGCTCCGAGCTATGCTCCAACTTGGTGATGTGCTCGTGACCATGGCGACGTCCGGTCCAGGGATCTAGTTCTAAAATCTCAAGTCCCAGGAGACGACTAATCCTTCCAAGCTTCTTTTGTGCCAGTTTGGATAGCTTCAGCGATTGATAGCTAACTCCTTTTGTATCCTCCTCCTCATCTATAAGTTGCAGCAATTCATTGGATTCCTTCGGCGTCAATACATCGTGATGCAAAACTATATATGGATCTGAACTGACAGGCTCCAATCTGGAGGGCTGGAGGAGCAACCAGGGCGAACTTCCATCCAACTGGCAGTGGTTGAAGCGGCTTCCTGTTG

 

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