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CRIMIC CR02130

Gene CG3308

CG3308

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3R:21271078..21273817	93D2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCAGGAGTACATTGCAGAAGTCTTCC	TGTCGTTCAGTTCCATGTGGCTCCTT

Images

Construct Design Sequences

sgRNA sequence	GCCCGACCTTCTGGCTGTCTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTTCGCCCACCTGTGTAAGT	CCAGAAGGTCGGGCTATTGG	CTCGCCCCAGAACGAAGAC	AAAACTGAAGGTGGGGTCGG

 

Amplicon 5'

Amplicon 3'

CTTCGCCCACCTGTGTAAGTACAACAATGAGCGTTCGGTGATACCAGTCTTCACGTGTTGCGGTAGCTTGGAAGCCCTTGTGTTTGGGTACATGAAAGGAGCATCCGTCTCCACCAGTAGTCTGTCTAGCGGCAATGTGCCGTCCTCCAGCAGGCGGCGAACGCCAGTATCCGATTTGTCCTTGCACAGGTAGCCCGTCAGGCTGATGTAGAACCTCCGGTCTAGGTACTTAAGCGCCTCCTCGGCGGTGCCCATAAAACCTCTGATGATCACAGGCGGAAGATTCTCGAATCTGCGCGGGGAACAATTTAAAGTTTTGTAAACCAAATCTACGGCTCTTACTTGTCTAGTATTTCGAGTAAATCGAGCTGAGCCGACCGCTCGTGGATCAGCAGAGGTTTATTTAGCCGGATGGCCAGATGCAGCTGCTTGGCGAAGATCTGCTTCTGGGCGTCCGGTTCGGAGAAGTCCCGCTGGTAGTCCAAGCCGCAGGGGCCAATCGCCACACACTCCTGCGCCTGGGCGATGTGCTCCAGATCGAACCACGTGGCCGGCTCTTCCACGATGGACTTGGAGTCGTGCGGGTGAATGCCAGCAGTTGAGTAGATTATGTCGGGATAGATGCGCGAAAGGCGCAGCGCTTCCTTGCTCGATTTGACCGAAGTGCCGTGAACCATTAGTTTCTGGACACCTGCAACAAGAAAGGCGGGAGAAAGTTAGTGCAACGTTCACTGGGCTACAGACTTGTGTACAAAAGAGAAAATATGTGACTTCTGTTTGCTTTCGATCAGATACTCTTATCAGCATTGTAATGTATCCCTTTAAGTAAATGTATTTAAAAAACAAATTTTTTGTTGAATATCAAATCAAATATGTAAAGAGAATTTATCTCTTACATATATTTAAATGTTTATAGTTTCCTTTAAAAATTTATCCTATGCAAATATTGTTGTGTTGACATCTTGGTTATTTGGTTTTTAATCTCCAGCTACGTTCCCATTGAATTTGTAATGCCATCTCCCAGGGCACATATGTACATGCATACATATATCAAAACACAGGCACACTTGTTGCTCGCCAATAGCCCGACCTTCTGG

CTCGCCCCAGAACGAAGACACTGCCCGGGTCTTATTAATTATGCCTGCACATGTAGTATCTGATGCTGGCATACAAACCTGCGTCTCTGGCTCTCTGCACCACGGAATCCAGATCACGGCTATACTTCTTGTTGGTCAGATTCGCACCCACGTCGATGACGATCAGGTTCTCAAAGCAGTGCTTCATGTCCAATTCCAGCTGATCCTCCAGCAGCGGGGCCACGGGGGCCACCACCGTCTCCTGGGGCGTCTGTTGACTGGGGTCCATGTTCTGGATCGGAATCTTGGATCTATTATCGACGAAAATAGCTTATGACGCAGGCGATGGATTGCTGGGCTGCGACTGTGTGCGTGGGTAATTCAATTAGCTTGTTTTTGAGGGCGCAAGGAAACGCGACGGCGGTGAAAGCCGTGAAATGTGGATCTACTGAATTTGTTGGAATTTGGAAGAGTTGACCAATTCGGCAATCAATGGTCTAAAAGTTGAGAAATATTAATCTAAAAAACAATCCCGACACGAACTACAACTGCTGGCTAGAGTTGAACAACGTCTGCAGAACGGGTCAGTGCAGGTACTACACTCTGCATGCAATCGATAGTCGGAATCGATACGTAGCACCAAATTATATCGTGTTTGAATTTAAATTGTTATCAAAATAAATATTATGTAATAAAATTCAGTCCAATTATTTGATATAAATATATTTGTTTCATGTATATAGTAGAATTCCGATCATCAATAGTAACATCTTAAGACAAATAATTAAATATTTCACAATACCATACCAGAAGTCGCAACTAAATATAACTGAGTCGAATTTTTTGTTTCGGCAACAGTCATTGTTTTTGGGGATTTAAAACCCAAGCCCACACACTTGCACCGACCCCACCTTCAGTTTT

 

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