CR02131

CRIMIC CR02131

Gene CG42233

CG42233

Information

Donor Location Cytology FBti Stock number
pM37-p13R:31799634..31805278100D2FBti0214967 Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
no band OK AGCACACACACATCTACGTGTAGCGA CCAGTCGCTCTTCTGTGTTTTGTGGT

Images

Construct Design Sequences

sgRNA sequence AGAGCCGTAAATCTAATTCTTGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
GTTTAGCAGTCCGCCCTCAT TTACGGCTCTCAGAATAAGCC CGGATGGTTCACGGGCTAC CCGGTGGCGGTACACTATAC
 
Amplicon 5' Amplicon 3'
GTTTAGCAGTCCGCCCTCATGTGTCAGTGGCTCTGACACTAAACAAACACTTGTCCAACAGGATGTCAGTCGACCTGCACCCTAGTATCCCGAGCCCATGCTATTGTACGCTCCACAATCTCAGTCGACGGTTCAGTAACTCCTGACATGGCGACGACTTTGCAGCTAATTATGCTGGCTCTAGTGGGAGGAACCCTTGGTCAAGCCAATAACACGGACCACAAGCAAGTCCTGACCTCCATTGTAAAACAGCTTGAGGGCGGCCTTGAACTGCATCTAAGGACGTCCGAAGACGGAGGCAATGACCTTGTTCAGTTTTTGATGCAAGAAAAGAGCTCCATCATAATCAGTGCAAAACAGGAGGAAGTACCGTCAAGAGCAAAAATAATGAGACATCATTTTTTCATTTTTGATGGCGTACATCAAATGCAGGAAATCCGGACTAGTTTGTTTAACACAGATGGCTTCTACATATTGGCTTTAGAAAATAACACAATTGAGGACGATGTACTATTAATGGAGTTCGCGGCCGATGTTTGGCTTCAGCATGGACATAGCCGGATTTACTACGTCCAATTAAGCAAGAAAAGTGTACTCCTCTTTAATCCCTTTTTACAGAGGTTAGTGGTGGTTCAAGATTCCAAAACGTATTCAAGAATCTACAAGGATCTGGAGGGTTACCATTTGAGAATATACATTTTCGATTCAGTTTATTCAAGTGTAATTGGAGACGGCGAAAACAAAGTCCTAAGTGTAACAGGAGCGGATGCTAAATTGGCCAAAACCGTTGCGAGGCAACTGAACTTCACAGCTGATTTTGTTTGGCCGGATGATGAATTTTTTGGCGGTCGGCTGGCAAATGGAGAATATAGTGGCGGCGTCGGTCGCGCTCACCGCGGAGAGGTGGACATCATCTTTGCCGGATTCTTTATTAAGGACTATCTTACTACTCACATCCAGTTCAGTGCGGCCGTTTACATGGATGAGCTCTGCTTGTACGTGAAAAAAGCGCAGAGAATTCCGCAATCCATTTTGCCTCTTTTCGCTGTTCACATGGACGTGTGGTTATGCTTTCTGCTAGTGGGTCTGCTAGGAGCCCTAGTCTGGCTTATTCTGAGAGCCGTAA CGGATGGTTCACGGGCTACGCGGATTAGTTACTTCGGAGCAGCGCGACGCATTTTCGTCGACACATGGGTCATTTGGGTGCGGGTGAATGTGGGTCGTTTCCCCCCCTTTCACTCGGAACGCATCTTTGTGGCTTCTCTGTGTCTGGTGAGCGTGATTTTTGGAGCCTTGCTTGAGTCCAGTCTGGCAACAGTTTACATAAGACCTCTTTACTACCGAGATGTAAATACCTTGAGGGAATTGGATGAGTCTGGCCAGCCAATTTATATAAAACATCCTGCCTTTAAGGATGATCTCTTCTATGGTCACAACTCAGAGGTCTATCGTCGATTGGATGCCAAGATGATGCTGGTGGCCGAGGGCGAGGAGCGACTTATTGAGATGGTGTCGAAGAGAGGAGGATTCGCCGGCGTCACCCGATCCGCCAGTTTGCAGTTGAGTGACATTCGATACGTGATGACTAAGAAAGTGCACAAAATCCCCGAGTGTCCCAAGAACTATCACATTGCCTATGTGCTGCCGCGTCCCTCGCCTTATTTAGAGGAGGTGAATCGGATCGTACTGCGCCTCGTGGCGGGGGGAATCGTCGGCTTGTGGACAGGGGAGGCGAAGGAGCGCGCCAAATGGAGCATCCAACGGTTCCCCGAATACTTGGCCGAACTAGACGTAGGACGGTGGAAGGTGCTCACTTTGTCCGACGTCCAGCTCGCCTTTTACGCCCTCACTATTGGATGTCTACTTTCAGCTATTGTTTGCATGGCGGAGATTTTGTTGGGCCGTCAACGAAGACTGCATAGTCCCAAGTAATTAACCCATTAAATTACGATAATTCTATTAAAGTCTACGTATAGTGTACCGCCACCGG
 
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