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CRIMIC CR02133

Gene CG7091

CG7091

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3R:12978000..12980535	87D6	FBti0213467	91448

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
long band	OK	CGCAGATTCAAGAGACTCAGGCAAGT	GCATCCAATCCAGATTGAGTCCACAC

Images

Construct Design Sequences

sgRNA sequence	GATGCTACGGAAATCTATATAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTTCCATCTCGCTATCCGGC	TCCACTAAGGGAAGGTGTTCT	TCCGTAGCATCTCCAGAAACA	GTTCTCAGCCGGGACTCAAA

 

Amplicon 5'

Amplicon 3'

TTTCCATCTCGCTATCCGGCTATCTGGCAGATCGGTGCAGCAGCAAGCGGCTGTTCATCGTGTCCCTGATCTTCGAGGCTGTGGCCTACATACTGCTCCCAGCAATGGCGCACTCTAGTTTCGAGGCCGGAGTGGTTGATCTGGTCATCTGTGGTTTACTGGCGGTAAGGAATAGCATCATTCAGATACTAATAGATATATATAGATCCTGCTACTTGACAGGGCTGTGGAAATCCGGCAATGTATAAACTGTTCGTGACCTGGGCTCATCCCACAGAGAGAACAGCGCTCCTGTCCTTCGCCTACAGTGGTCTTCTGATGGGCTCCATGTTGGTTTACCCGGTGGCCAGTTATCTGAGCAACTTCGGCTGGGAACTGTCCTTCTATGTAGTCGGTGGAGTTGGCCTCTCATTCGGCATTGCCTGCTGCTTTCTCGTTTACGACACCGTGGAGCAACATCCAAGGATATCGAATGAGGAGGTGGATTACCTGAGGCAGGGCAAGAGTCAATTGGGACAGCAGCGGCAGCCAGTGAGTAGTTCCTTGTACTTTTAACTTTAAAATTATAAATTAATTAGCTTCTTTTTAGGTGGTAACCATTCCCTGGAAGAGCCTGCTCGCCGCGCCGCCCGTCTACGCCTTCATCCTGACCCACATGTTCCACACCTACACCTTCCTGGTGATTGTGCAACTGATGCCGCGATTTATGCGCGAGGCCATGGAGTTCGATCTCCGCGAGGTGGGCTTCCTCTCGGCGGCGCCGTATCTGGGCGGGATCTGCTCGAAGGTGATGTGCATCCTGGGTGGCAGCTATGTGGAACGACGCGTGGGTCCTGACCAGAATTGTGTGCGAAGAATGCTGTACGGGATCTGTAAGGCATACCGATAATCGGAGTGCCACAGACATCTATAAAGAACACCTTCCCTTAGTGGA

TCCGTAGCATCTCCAGAAACATCACCAATCTCTCTTTTATATCCCATTTTATAATACCAGGCAGCATCCTGACCACTTCTTTGATTGGGGTCATCATCCTGGCTAATTGTGATGACAAAATACTAGTACTTGTGATGTTTGCCTTCATGATGGCCACTACGGATATGGGTTTCAGTGGCTACTGGCCCACGCTGCTCTACTTTGCGCCCTCCTTCGCGGGATTGCTATCGGGATTGGCCAATGGAATGGCCCATCTTTCCGGATTCTTAGCTCCCCATCTTGTGGCCGCTCTGGTCCACACTGTAAGTTTAAATACAAGTAGTTTATGATTAAACTGATTTATTTATGTACCTCGCAGGGCAGCAAGGATGAGTGGAATGTGGTGTTGATGACGCTGATTGTCTTTAATACGATGGCCATGTTGGTATTTGCATTTTGCAGCAGTACGAACCTACAACCTTGGGATCCTCGTAGCAGAATGGAGAAAACTGCATCACCAAGTGCTCAGGAATCAAATAGTTAGCACTTATTTGTTTAAATGTTTTAACTTTTTTGTACTTTCGAAGCATTTTATTTGTTTACTATAAATGTAGAGTTAATTGTGTTAAAATAAAAACAAAATAAATGTAAATATACAATATACATATCAATTTAGTTTAAAATGATAATTGAACTTTCTTCAACGCAATCTTCAAATAAAATTTTTAAAAACCGCGCCTAGCACATAGTATCTTTCAATCTTATCGATAGTTTTACCGTTGTATAAAGCAAGCTGACAAATGTTTCACAACGACCAGTTTTCTACATTTCAAGTACAAAATTACACAAATATTCAATAATTCAAAAATATGTGTGAAAGCAGACATCTATTGGCAGTCAATGTACTAAATGCTCATATTCTTTACACGCCAATCGATAGCTGTTTGATTTGACGCATAGATTTGGTTTGAGTCCCGGCTGAGAAC

 

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