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CRIMIC CR02134

Gene CG7523

CG7523

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3R:17669144..17670895	90C1	FBti0213468	91449

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
long band	OK	CACACTCTTTGAGTGAGGGAGAGTGA	AGACGAACAAGGTGGTGGAAAAGCCT

Images

Construct Design Sequences

sgRNA sequence	CAGAGCCGCTTAAATAATCCAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AACGGATTGCTGCTACGTCA	TGAAGTGATTCAGTACAGCCACT	AAGCGGCTCTGACTCATTTT	TGGAGCTTTCAAACGACCGA

 

Amplicon 5'

Amplicon 3'

AACGGATTGCTGCTACGTCACGTATTTATTGCAGAGGGTTTAAATATCTACAGTCTGCTTCTAGATGAAGGACACATTTATTGCGGAACGCAGAAAAACGAGTTATATCAATTGGAGTTTGTTGTAAGTGGTTAGAATATGACTTTCAGTTGGCCGAACATTGAATCCTGCTGTTTTCCGTTTTAGTCTGGAAACTTGGTCACCAAGTTCAGCTGTGGAAATGGCGCTGTTGCTGTTGCGGCCTACGGTGAGCGTTATTTGCTAGTCGGCTGCTACGACGGCTATATTTACGTCCTGAACAAGATTACCGGAACTCAAACTGGTCGTTTCGCTGGCGCTGGTCGCATGGTTTTAGCCCTTTCGGTTGTAGGCGATAAGGTAAGTTATTGTATAATATTTCCAAAGTCGGTATTCAAATGTTTTACTCCAATCTTATAGATCGTCACATCTTCGAAAGATAACTCGCTGGCGATTCTGGAGGTGCCCCCCGCATTGGTAAATGGCTATTAAGACGCGTATCATCATTGATATAAATCTGCTTTAATTTAATCATTTTTACACACAGACTTGTTTGCATAATACAATTTAATGGTCATAGTCCTTGATTGCGGATTTATAATTCGTTCTCTGTATTTACAATTGAATATTACATTTTTTGCATTTTTGCAATTGATCTACTTGAAGAATATTTCTTCGCCATGAACAAAGTATAAACGAATAAAAAGAGAACATCCAGTAAAATTATGATACGTAAAATTATATAGTTATTTGATTTGTCTTTAAGGAATTTGTGGTGCCCTCACTGGTCGCACGTTGGTCTGCATTTGATGCAGTTTCTGGTTGAGCTTGTGTGTATGGTCACGCAGATAGGCGATAAGGTCGGCCTGCTTCTCCAGAAGCTCTTCTGTGTCGCCTCCTGGTTGCATGAGGCCCAGGGAACCGCCGGCGCGGTCCGCACCACGCAAAACATCCGGAGCCAGCTTGGCGCTGTTGAACTTACGCACCTTGTCTGAAATGGGTAACGAATTTTTAGCCATGTCTATGACAACTTTTATACCTGAAATTACTTGGTTCTTAATCAAAAATCTTTCGCTTTGGAACCATAAGAAAGAACGAACTTAATTAGTGGCTGTACTGAATCACTTCA

AAGCGGCTCTGACTCATTTTTTGCGTATTACAATTGCTTGCAAGTCACTTAATCAGTTTAAAAAAAATACCTAGCCATCCCGACTACTATATTATGTCATTATATAAACTATTTGAATAAAATGTTGCCCATCGTCATTTCAATATATATACACACAGATATATTAAAAAAATGATGATAGCTCGATCTGTCATAAAATAAAATAAAAATAAAAGTCATACCATGACACTAAGTAGATTCCCTCACTATCGCTGACTAGATACTATGTTACTTCATTACCAATATATAAGCTGTGGGAAACGGCCAGCACCAGATTCTCGGCCACGGTGAAGGCGGTCACATAGCTGACCGGCGACAGCCACCCGGCGGCGCAATGTTCCCAAAAGTTCTCCGCATAGAAGTGGTGGATTAGGGCCTGGACAGCAAGCAGCATGGAGTTCCACAGGGATACCAGCTGAAGGGGAATCCCCTTCTGCATGTTAGTCTCGCGGTGAAAGTCGTGATAGCCCTGCATGCGTAGATTGTCGTGTTGCTTCTTTACGAAGTGATCGAAGAGCTGTAAAAACCGTTGACATTGAGGCAGGTCGGGATTGACAGACTGCTGATTGGTATATTTACATATGTGATGACCCAGAAGGTGGCCCGTAGGTAGAGCATTATAAAGTATGCATCGCAGCGCCGATCGGTTGGAAACGAGGCTGCAAGTACGATCCCCACAATGGAGATTGCAGTGGAGATAACCAGATGAAGGCTACGGGTGGACAGAGAATGGATTAAATTATGTGCATTATCGGTCGTTTGAAAGCTCCA

 

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