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CRIMIC CR02135

Gene CG8176

CG8176

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3R:9516007..9527237	85D19	FBti0213469	91450

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
long band	OK	CTAGCGCTTTCAAGAGATTGAGGAGG	CGCTATTTCCTTAGCAGGCTGTATGC

Images

Construct Design Sequences

sgRNA sequence	CTATTGGTTCAATGGCATTATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGCCAGCGGTAGTATACCCT	TGCGTGTGGATGTGCAGT	ACCAATAGCACTTACAACTGCA	GTCATAGTCCAGCAGCGTGT

 

Amplicon 5'

Amplicon 3'

AGCCAGCGGTAGTATACCCTTTGCCGAGGATCCGATACTGGGAGCTATGGGCTCTCCGCGTCCCACATCGCCGGACATGCTGGCGGGAGCCAATGCATCGGGAATACAATCGCAACCGACTGGTGGAGCCACATCCACGGTGAAAAGCCTGCGATCCTGGTTTTTGCCCACTGCCAAACAGCAACCGTCTGCAGGCGCCGGAAATTTCGGTATGGCCGGAAGTGGCAGCACCAACAATACCACCACCACGACAACAGCAAGCAACAATAGCAACAACTTGAACACCAACAACACAACAGCAACAACTACAACAACAACTGGCACGAATAATCTTACCACAAATATCACCAAAAATTCCCCTAATTCTGAGGATACTAGCAATCAAGGTTCTTTCTCTTTGATTTTATTTCGTTTATGTTTTTGTTTATTATTTTTCATTTACATCGTTGTATGGTTAAAGTTTATTCCATGTATCTACTTGTGGGGCCTATCATTTGTTCTATCAATTTCAACTATTCTTCTGTAAGATATTTCCTTATATGTTCTTTTGTATGTATTTACGTTTATGGTCGACTCTTAATTGTACCTATTCATGTTGCCTAAAGTTTTAAATTGGTTATTAGAAATTGTGGGATCTAATGCTATGTATTAATTTCACGAGTTAGCATTAGAAAGTAGTATTTTATTCATTATAATTGTAAATAATTTTATTTATTTTTATTTTACTCATTTCTTAGTTTAATGCGATCTTTAAGTGTACACTCAAACACCAGCCAATCAATCAGACAATTCTCCTGACTTTTACACAACTACTACTGCACATCCACACGCA

ACCAATAGCACTTACAACTGCAATCTTCTCTCTATGCTTTTCTCTGTCTTATTTCCGTACTAATGCTTTGCACACAACCATCGCATCCATCAACCACTGCTCATCCAAATCATCCATCATCCAATCATCCACCCATCCGTGCACCGATCGACTAATCGAAATCAAAACTTTAGATCAGACACAACAGCAACCGGCAGAGGCCTTAACTCCAACCGCAAGTGCCACTGCAATTGCAGCGGCGAGTGCGCCTGGGAAGAAGCAATTGAACGCAACGACAAGCGAGCAGCAGCTATCGAAGTCATTAAATGAGCAAGAGCACCTACAACAGACTGCAGCAGCTACGGCAGCCACAACTGCAGCAGCAACAACCGCCACAACGACAACGTCCAGACGTGCAACGTCACTTTTAAATATATTCACATCAAACTCTCAGGGTAAATGATTTAAGAATGTCAATGAAACTGATTGGCCCCCATTTCACCACCCGTCCAACTTCTAAAACACCTGTACAACATTTCCACCCGAAAAAATTGCATGTACCTATCCTTAATATGTATATTTAAAGGCTTTTCTGTGTGCTCAACTTAGTGTTCTCTCATATCCGATTAACATCTAATTGTTATTGAAGCACTTTAAGGCGGTGTTTGAGTTCTGAGAAAGACAATGCATTTAAGTTTCAACTATATATTCCCATCACCGAAGTCTCAACACAGAAGTATATTGATGTGTTGAGGTGTCATCTTCTTTTCCTGTGTATTCTATTAGCCATTAGCTTGCTTATTTACTTAAGAATCTCTACTCTCCTGCTTTCATTAATGTGATCCACCTTTCGCCGGTAGTCTCTGGCATTTTACGCAGTCGTCGCGACAAGACAAAAACCAAAAAGGCTAAGAAAAAGAAAGACAATGAGGCCGCCGAGAACATACAGGAAGAACGCCTCACCAGGTGGGTACACAAAATATTTATGAGGCTCAATTAACAAAATCCTAAACATGAACGCTCTCTTAATTATTTTCTAGTTTGGATCGAGGCAACAACACGCTGCTGGACTATGAC

 

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