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CRIMIC CR02138

Gene elm

CG2185

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3R:5652640..5654861	83B8	FBti0213470	91451

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
long band	OK	TTGCGAGTTGATGGCTGCGAATCTCT	CGACGGGTTCCTGAAACCTACAAGAT

Images

Construct Design Sequences

sgRNA sequence	ATTGGATACAAGTTCCAATGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AAGGTCGCTCTTTCCTCGTG	TCCAATGTTCGACCAGCTAGT	GCAATTTCAATTGTGCGCTTAT	AGTCCGTCTCTAGTGCAACC

 

Amplicon 5'

Amplicon 3'

AAGGTCGCTCTTTCCTCGTGATTGCCGCATCCAGCTAGCCTAGTTGAGAAACCGTATGGACATCTTCTGATCCACGTCGGTGCGGTCCAGAGCTTTGCAGAAGTCCTCGAACGAGATCTTGCCCTGGCAGCACAGGTCCGCCTCCAGGATCGTCCTCTCCGCAATGCTGACCAACTGGTCCTGGCTAATGTTCGCCCCGACCATCATGTGCAGGATGGAGAGCAGCTCGTCTCTGCTGATCACACCATCGTCATCCAGATCGTACATCTTGAAGGCGAACTTCAGCTTCTCCTCCCGGCTGTTGAGCTTACTTTGCTTGTTATCCCTCAGCGGCCGGAAGTGGGCCAGGACGTTCATGAACTGCCGGAAGTTCACCCGATCGTCGTTGCTCTCAGCGAAAAAGGAATGAACTATCCGCTCGCACAGCGGATTAATGGCCAGTTCCGGGATACGCATCAGATCCTCCCGGGACAAGGTTCCGCAATCATTGCGATCCAAGGAGGTGAAGCGCGAGTAAAGTCGCTCGATTTGGTTGGGGGTGACTGGAAAATAAAATTGATAAAAATTAACCAATCGATAATTAGTTTTGAATTAAATACAATTAAGATAGTACTAAAACATGTACATTCAACTGTAAATACCTATATGATATTAACTTTAATAAGCGATTTAACAGTCCGTCAAGTTAAATCATTTAATAAAGTTGTTTTTGTTGAACGACACTGTTCAGATAAATTACTTTTGCCATGGAAAACTCGAACGATACCTATTTACGAACAGTGTGGGCATGAGTCTAAGCCCGTTCGGAATTAGTTGCTATAAAATCTGGCTGATAAGAGCGAACGCCTTATCATCTTAGCTGATACTAGCTGGTCGAACATTGGA

GCAATTTCAATTGTGCGCTTATCGCGCCCGGAATATTGAAATTACACGCTCTCGGACACTAGATAATTGCCAGCCCCAATATCGGTGATAGGCGTCGACTTTTGCCCCCACCCACCCCCGTCTCATATATACGTATATAACTATCGATGACGTCATGTGGGAAGTGCAGGTGCATTTGAAATGCAAATTGCTTTTGCTGCAAACCATCTGTCCATATACCGCTTATATAAGGCGGGCACAGATCGCTCAGACTCAGTTCTGCATAATTTGAACGGAGTTTTTTAGAACACGTACATATAGTTCGTAGATTTGGAATTTTTATAGAAATGACGAATCGAAACTAAGCGAATCCATTTTGGCAATCCATGGTCTTGGACCCGAAATGCTTTTAGCACTCCAGTGCGATGTTGTTGTTTGCATTGCTCGTGGCCCAATTAATTTATATCCAAGTGCGTATTTCGATTTAGTACGGCGCATTGAAAGTCTCCGGTTGAAAAAGCCCAAGGTTATCACACATCCCGACCAGAAATCGTACTCACAGCCAGTCTCCTCCTGGATTTGCGCGATCTCCTCGTTCCGCAGGAAAAGCGACGACTTATTGCCCATGGTGACGGTGTGTTGTGCGCCGATCGGTTTGTTTAAACATTCAACAATCGCAGACGAGCATTGAATGGCATTCGATTTGGATTGGACCACAAGTCCGATTTGTGTACAGGCTGAATGCAGGCGGAGTGACGGGAAAATGCCGAAAATCGGGTGCAAAAAAATACTGAACAGCCAGCGATGGTTGCACTAGAGACGGACT

 

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