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CRIMIC CR02139

Gene ema

CG12753

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3R:16436880..16441661	89B16	FBti0213471	91452

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CATCCCGAGTCAATGGTGAGAATTGC	AACTGCTGCCACTGCTGTTACCACTT

Images

Construct Design Sequences

sgRNA sequence	CATGTCCCAATCCGTTCAGATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCCATGCAACGGATGTGATC	ATGTAACTCGTCCACTGCCC	ACGGATTGGGACATGTACCA	AAGGATCTGAACGCGGTGTT

 

Amplicon 5'

Amplicon 3'

GCCATGCAACGGATGTGATCGTCGAACAGGAACTTAGCCGAGAGCAACGGAGTGCGGTTGTGAGTGACTCCGCCCCGGTGCACGGTGATGTGCAAGCAGCGCGAGTCATCCTTATCACCCTGCACCTCTACGTCCTGCAGAAAGCCAACGAACTTTGCCACGCCCCAGCCAAGCAGCTTTGCATCCGGCTCGACGAGAATTAGCTGCAATGCGTCAATGACCAGAAAGCGTCTTTGCTTGCTGCTGTCCTTGGCCACAACGGTGCAGGCTATTAGATCACTATTGTCTAGATGGATAATGGTTAGCCTTAATATATACATACATCATCGTAGGAGGAGTATACTTACTCAGATCGAGTACGTTCTCCACCTGAACCAGATTGACTACATTTGTAAGCGGCAATAGCGATTCCTTCTCATTCAAAAACTTTTGGCACGTGCGACGCAGCAGGAAGTAAACGCGAATGGCTCGGCGTGCCTTTTCCACCTCCCCGCAGGGAAGTCGTCGCGTAAAGTTGATTCCAGTAAGTGGCGTTCCCGTGGGAGGCAGTAGAATGGTGGAGTCCATGCACAGGAATTCCACGTTCAGTTGCGCCTTTCGCATCTCGTTGTATTCGTCTTCGAACAGGTCTAGGAAGATGTCCTCCGACTTGTAGAAGTTACGGATCACCACCATCGACTGATTGCGGGCGCTGAAGAGCAGATCCTGTTGTGCGGCGGTTATGCAGCGCGAATCATCCGAGCTGGGACGGGTGAAAGTGACCAGTAACTCCAAGGCAATCTCCACGGTAATCAACCGAATGCGGGACGCTTTAGGGAATTCAAATGGAATTATTAAAGAGCTAATGATAACCGGAGTTCGAACAGTTGTACATACATGGCTGACTGGACTGGGTGATGATGTTTAACAGATGCTCAATCAAGGCTGTCTTATAACTGAAGGCGCCACGGGTTGATTTTGCAAGAACCTGCTCGAACCACTCATTCTTGATGCCTATATGGAAAGGTACATAAAGATATTTATTAAGGAGACTATGATAGATGCATACGATTGATTAGTACGGGCAGTGGACGAGTTACAT

ACGGATTGGGACATGTACCAGAATGTGACATTTTCATTACACACAAAGAAAGCAATTAGAATGAAGCAAAAATGGGGTGATCAGAGCAATGGAAAGTTACACAACATTAGCCAAAAAAAACGTCAAATTGAGTTAAGGGAAATATCCATGAATATTACCTCTATTGTGCGACATAGCATATATGAGGCACAGCGACAGCAGGGCAAGGTAATCGTTTTCGGTGCAGTCCAAAGCATGCAGGACCGTGTCTAAAAACGGTTTGGCCATCTCAGCAAAGTCAGCCTTCGTTGAGGAAGATGACTTCTGCAGCAGCTGCTTTTCCTCGTCAGTTATGTTGCGCAGTTTAATCTGCTCCGCTTCAACCGCATCTGCTTGAGAGTCCAGTTCTGTGGTGGCTGGGGATGAGGACTCCACGCCACTGTCCTCGCTAAGGGCATAGCTGGACGAGCTGGACTGACCGGTGACTGTATCCAAGGCCTGTTCGAGACTCTCGTCGGGCTCGCCGAAGCCAGGCACAACCACCTCGCGGTGCTCAACGTATGAATTAAGAATCTCGGCTGCACCCTCCTTAAAAACGCTGTGGTCACCATTGAGGATAACCCAGGCCAGAGCGTGCACCAGAGGGGCATGCGAAACCACCAGGAAGACCAATGAGAGCAGGAAAAGGGCCACGATGGAGCTCACTCGCGGATTGTGCATCTCCTGTATGTCGATGTCCACATTGCGATTAAGCACAGCAGCTAGATTCTGGGTAACCACTGCCAGTGAGGGCGGGGGCGGAGCTGGTGTAAGTGAGAATATGTACAGAGGCACGAATAGCTTATGCAGCAAGTGCTCGGTTAACACCGCGTTCAGATCCTT

 

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