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CRIMIC CR02140

Gene glec

CG6575

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3R:21850694..21856266	93F14	FBti0214968	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTTCGTTCATTCACGTGCGTAGGGAT	GCAGAATGCAGTCGTCTGGAGTGTAT

Images

Construct Design Sequences

sgRNA sequence	ATTTCCTATAGCGCGCCAACTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCGCAGTGACCTAAAAGCTG	GGCGCGCTATAGGAAATGC	TGGGTGGTAAAAGTACAGTGGT	GAATAACAATCGCGCGCCAA

 

Amplicon 5'

Amplicon 3'

GCGCAGTGACCTAAAAGCTGGAAAGCTGAACAACAGCAAAAACATGAAGGCCCCAAGTCTAGCTTACATAACCGAATACTTAAACACACTCGCAAAGTAATTTGTCTATTATAATTAAAAAATCATGTAGGTGCTTCATTTAGAATTATAGAACTATGAATTCCAACGAAAGTATCTTGCTATTACGAAGCAAACTTATCATTTCGAAGTCAAGATAAATACATACAGTTAAGATTCTATGCCAAATGTATCCATTAAAGTGGAACGAAAAGCGCAACAAGTGAAATAAGCCTACTTTCTGGACGATACAAACGTGTTCCTTAAACTCGAAGCCCCCCGAGAAAGAGAATGGAAATGGCTGGCAGCCAAAGAGTCGCGCTGCTTCTGGCGATATAATCAAATGTACAGTTATAGAAAATTATAAGCCAGCGACAAAGAACGTCAAGCGAAACAGAGGAGAAGAAGCAGAAAGGAAGGAAAAACGGCAAAGCGATCAAGTCCATTGTAAAAAGCAGGTGTTGAGAAAGAGAAAGGGGGAGTGTGAGGGCGGTAGTGCGTTTACCGTTATCATTATAAGCACGAAGCTCCAACTAGCTCTGTCTCGCTCCATCTTGCACAACAAAAAAAAAACGCGTTTTGTTTACGAAATGTGTGTAGAGAGAGAGGCGGCGATGAAAACAAAGAACTCACATTATAAATATTTATGTGGGAGCCCAAAAGAGAGTTTCTGAATGCATAATTAGAAAACATAAATTTAGGGGAATATCACACACATTTTTGGGGCGTGGCGAAGAAGGCCAAGCAAAACATAATCACGAAAGTCAAATAGACCGCTTAGAAAACTTAATTTACGTGGTAAAAAATCCACACATGCAAATGATAATGAAGATGCTGTAAATTGTTAGTAAAAATATGCAAAATACCGTAATATGAATTATCATATCATAGGAATATGTATGCATTTCCTATAGCGCGCC

TGGGTGGTAAAAGTACAGTGGTGATCAAAACATAGCAAAAAAGAATATAAACAAGATAGGAATAAAGGCCGAGATAACAGAACTTTTGTCTAATTGATTTGTTTTTGTTCGCTTCGCTTTTCGACCCGTTTTTGGACCTGCCACACAGTACAACAAAACACAACATTTGGCGCTGCTGTACTTACGCGAATTGCGTTCGTTGGTGTGTGTGACCTTCACGACGAGTTTTTGAAAAACATCATCAACGTGCGTGCGTAGTTACTACTACGCATAATAACGCACACACACTCGCACACGCAGAAAGCTTATCAGCGAAGACGACTAAAAATAAACACGAAAATAATAAGTTCGCGCCAAAAACCAGCAGACAAACAGAGAGAGAGAGAGAGAGAGAGAGCGACGACTCTGACTCTCTCGTCTTTCTTTGATAATGCATAAGTGTGCGTGCGTTTGTGTGGAAGTGGGATGACGTCGTCGTATAAGAGCTGTGGTTTTCCTCTGGTTGAGATTGCGTTTGTCGTTGTGGGTGCGGCCTTTTCAACTAACTAACACAACTACAGTGAACTCATGTACAGTCGCAGGTCTGTGTAGCTCATTTTTTGTTTTTTTATTTATTTTTTTTTTCTGTGTCGAAACTGCAGCAGGAATAAAAACAACAATCAAGAAAACCAGAAGGCGTTGATTTAAATTTAATTGAGGCCTCTCAATTAATTGCCTTTGCATTTTACTCATCTCATATTCACTTTTCAGCTGCAATCTCTTTTCGCAAAATATGGAAAGATTTTTGGCGCGCGATTGTTATTC

 

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