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CRIMIC CR02142

Gene HHEX

CG7056

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3R:21076862..21080926	93B10

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCAGGAGTACATTGCAGAAGTCTTCC	TGTCGTTCAGTTCCATGTGGCTCCTT

Images

Construct Design Sequences

sgRNA sequence	TAGGAGTATCGTCGTGTATAGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTTTCCCTATCTCCGCCCAC	TCAACTGTACTGCTATCCCGA	CACGACGATACTCCTATCTGCA	ACCTACCCATGCTGACACCT

 

Amplicon 5'

Amplicon 3'

TTTTCCCTATCTCCGCCCACCCGCTCTTATCAAAGTAATCGGACACAACAATTGCGAGTGGTACAAGTGTATGGGTGTTGTATCAGTATCGGAGAATATGTCAACGCCCACTTGTAACTGGATGACGACTTGCGAATGGTGGCCATTGCGAAATGGATCGACTGTACGCCGAGATTTCGTAGCAATTGGGCGAAATCAATTGGCACCCGAACGGCAATCCGATTGCGAACCTATCTGCACCTATGTACATACACCTATGTGATAAGGAAGGTGGCCTCTTTTTTTTTTTTTAGTTAAGGTAACGGAAATTCGCACTTGGCGCATATTTGGAGTGTGCTCCATCGCTGAACTAAGCTGATATTTGGGGACCCAGTTACCTCATTGGTGAGGCGATCTGGCCAAGAACCCTTGGAAATGGCCAAATGCCATTAAATAAATAACATCCGATTGCAGATGCCATAATTTAAATCGTTACAAATAGACTTATTCATGTTTATTTTTATTAAGTGGCGTATTCGGGAGTGATAAGGCCGCAGAGCACCCTTATCTTCTTCCCAGCGGCATTTAATCGCTGAAAATTGGATTTGTTATTATTTTATAATGTGCGTGTCCGATTGAAGGAATAATATCATATCAGGTACTTGTCGCCAACACCACCACACCCGCCTCCGCCCCTAACCATTTGCCCCTTGTCCAGGTGGAACGAGTGTCGGGAACGGACGGGCTTATCGTAATCAAATGGCAATCGTGGCTATAAGATGATGGACAGCGCCTATTAATCGCCTCTAATAGCATTTTTGTTTAGATAAGCCAAACAAATCGGAAATCGGGATAGCAGTACAGTTGA

CACGACGATACTCCTATCTGCATTGTGTGATCAGTATGGCATAGAGCAAGCTAATCAATACTCCTTTTTTTTTTTTGAGGCGTAGTATGATCCTAACTCAGTTGGACTCATCATAGATATATCCTCAAGACGGGCATATTGGGAACAGAAAGGTGCACTTAGATATTCCATTCCACTATAATGTTGTATTGATTCCTTCCTATTTCACTACAATTATATCGAAAGATACTGCCTGTGATAATTGTGTGTTATCCAAAGGTTTAAATGAGTATGATTTCTCACAGTCCGTCTAATTCGCAGCAGCTCGATTTTCCCAAATCAACAGCTCAATGCCTTTCAATGAAAACTAACTGTCACTTAAATATCCATAGATATAGTTTCTGCTGTCTCTCACCTGAATTTCAAATATTTGTCACTTCTGATAAGCCACCGAAATATTTTCCGATCTGAATGGGGCATTTGTACTGCGAGGGGATGGAATTCGGGACTTTGACTTTATCAGCATTGCTAGGGCTAATCTACCGTTACACCACCGCCCAGAAAACCAACCGGGAAATGCAGTTTTTGGGGCGGGGAAAATGTGATTTCGAGTGGCATTGGCGCCAACACTTGACTTTGACAAATACCCAACTGCACCACAACACACACACATTAATCATGTTTATGGCCCATTTTGTGTGGGATCCCCATTGTAATATTCGGTGGTCCGGGCACTTCGGACTTAGGCTAGCTTAGATATTTGGTATTTAAATGGGGATTTCAGGCAACACCTTTCCCATGGCGATCAATTTCCATGTGTCAACTTGATGAGCGAGATTAAGGTGTCAGCATGGGTAGGT

 

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