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CRIMIC CR02149

Gene mthl5

CG6965

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3R:11883938..11887168	87A1	FBti0216171	92658

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATCATCAAGCCTCATCCTGGTGCA	AGTCATGGTATAGCGGTTCCAGACTG

Images

Construct Design Sequences

sgRNA sequence	ATGGCCAACCAACTTCGTTGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATTGGCCACATCTGCATGGA	CGAAGTTGGTTGGCCATTCA	TGGCAACTTTTTCGAGCGAC	AGCACGGTGATGGAAAGAGG

 

Amplicon 5'

Amplicon 3'

ATTGGCCACATCTGCATGGATCCACAATTTGGAATCCCGATGACGAATTTGAACTTAACGGGCCTGTTTTGCTCTCCTCCATAGCTGGTGAACATCGGCTGGAAGTAGTCTGCAAATGGAAACGGACGATAAATAGAGAACACAGACCGTGGAAAATCATAAAGGCAGTTCAAGTTAACGCTGCTTTTGTTGCGGCTGGTGGATCAGAGGGGAGCCAGATTTTCTTTTCTCTCTGCGAATATAAATATTTACGCTTCTGGGCAAAGAACTTGAGCTGTTCCATGCGCCTTCATCAGCTGGTTTTGCAATTTGTGCAGATTGGTTCTTCTTCAATTATCGGAGTTCGGATACATTTTTTTCCACTCAGCCATTCATATGTTAATCATAGCTAAGCACACGGAAATTAGATTGCATGTTGCGATTATGTGCCCTGTTGTATTGTAGTATTGGGATTACTCAAATGACTACAATTAAAATCGGATGAGATCAGTACAATGCAATGATTCAACTATCAGTAATAGTTACATTTTTATGTGTCACCTATATGGCCGATCCATGGTCATAAATGTTTTATCCAGTTTTAGTTGTCCGAGCTAGAATATCGATATAGATGCTAGAGTACGCTTTTCTATTTCCCACAATTGTTGCGATTGTTTGTCAAACATTCACCCAAGGCAATATATCACTATATTCACCATTAGCTCCACAAACATTAATTACCAGGGCTCGCATTTATGGGGAACAGAAGCATCTTATATTACCTTACTAATGGATTTTTTGCCTCATTTTACTTCCAAGAGATAATATTCCCGATTTGGGAATCCCTCATCAAGTGGCTACTTTATAGTTTAAACAGAAACATGCCCCACAGCATAAATCATATTTCTCATCAGGATTGGGGCCACCCTCTGTTTGTTTTAAGAACTTTCTTGGGGGAGTGCGAACTGTGGAGTTGGGGATATTTAGCTGTTGGCTTCGTTCCACTGATCCACTGATAGCCAACAAATGGGCCATTTAATGTTGGGCATACATATGTTGCATGCCAGAAGGGAAAATCCATAAAGTTGAATGGCCAACCAACTTCG

TGGCAACTTTTTCGAGCGACTAGGAGTTTTCCGCCTGGCCAGCTGTTGAACTTACCCGTTTCATTGACCTGCTGACACTCATGGTCCACGTGTATCTCGAACTTCTCGCAACATTTGTTGACCAGCACTAGGTTGGGATCGGAAGATAGGGCTGTTGATGATCCGGCGCTAGTGACATGACTGGTTACTGGTAAAGGGTTTAGACTAAGAAGTAATACGCAGAGCAGGGAAATCAGCAGAGCGCAGCAGCTGCATTTTTTTGCATTTTGCCCGGCTGCAAAATGCGCGCCAAGCGTTTTTACGAGCATCTTTTGCTTAGTAACACAGACACAGATACAAATACAATACACAGGTAAAAGGCAGTTGTCGTTGGTGTTTATGTGGATCTGCGCTTGCTAAGAAATGCGTCATCAAGGTGCCGGCTGTGAGCCAAGATCATGACTTGCATGCCAGAACTCAAGTAAGGCGACACTTTTTTTTGTATCAACACACCCACAAAGGCACTTTCGTCTGGGTTTCAGACTCGTATTCGGTTAATAAACAATGCGCGCTTTACTTCCTCTTTAAACAGTATTCAAAACGAAATAAAAATTGAAGAAGCAACATCAACCGATTCGAAGCGACGACAAAAAGCGAATGACTCTCTGTCCCCGGCTCTTAATATCGAGTCACTAAGGCGGCGAGAGAGTGGAGAGTTGCGAGAGCAGCGCTCAACCCCCACCAAAGAGCTCCCTCTCTCGCATTGAAAACCAGACTGAGCGTTGTACCCGAGTGCACCGACTATTCACTGCCCTGCCTGAAGGAATTTAAACCCACATATAATACCGTTCTTATTTATTTTCCGATTTAATACTTTTTTAAGGCATGCTATCAAATGTAGAAGAAGACATAGTAGGGAATGTACTTAGACATTTATGTAGGCTTACTACGGTTTCTATATGACTAAATGCTATTTCAATTTCAAGTTACTCACTTATTGCATTTGAAACAGATTGCACGCAAACAGATCTATATACTCAAAAGTAGCGTTGTATAATACCCTTTGATCCATCGAGGGTATAAAATGTGGAAGCAAGGCTTTGAATTCGCGCCCTCTTTCCATCACCGTGCT

 

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