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CRIMIC CR02152

Gene Nsun5

CG42358

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3R:19160099..19162300	91F4	FBti0213472	91453

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGGCTGATGGACTCAACGTCTGTTCT	TCTTGCAGCTGAGCAGCTTGAAGGAT

Images

Construct Design Sequences

sgRNA sequence	GGCTGTCATTGATTCTCCCGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCACGGAGTAGATGCATCCC	GAGCATCATACGCAGCAGGA	AGAATCAATGACAGCCGGGG	CAAGAACGCGCACACTTTGA

 

Amplicon 5'

Amplicon 3'

CCACGGAGTAGATGCATCCCTTGTTCTGCATCACATTGCATATGTGCACTGTCTTCATACCGGGAGCTGCGCACATATCCAGCACAGTGGCCCCAGACGGCGGAGCAAGTAGTTCGGCGGCCAAACAGGTGGCCTTGTTCTGCAGTATAAACCTCTTGCTGTGCACCAACGGATGGCGGACCCAGTAGTTGGACCACTTGGCAGGAAAAATGAGCACACCCTCAACGTGCAGATCGGTCATGAACTCGTTCTCCGCCAGCGATTTGATGGCAGTCAGAAAATCCGCATAGCTGGCGTCCGCAGGCAGCTCCTTGCGCCGCCAGTCGCTTTTATGCAGGTAGTCCAAGGCCTCCGCCAAGCTGTATAGATTTGTGTTGATGCGCACATATCGAGGATTGGGTTCTGTGTGGGCAAACAAAAAGACGAGTTCAAAATTGTAGCCAAAGTAAGATTTATTAAGAGTTGTTAAGAGTCGTTACTGGTCCTTTAAACGATCGAAATAATCTGGACTTGGCTGGCAGCACTTAAGTTACTGTTCTTTCTTTGGCTCCGCAGCTTCCGGCTCGTCGTCCTCATCCTGCTGCACGGGTTCCAAGGGTGGTGGGGGCTTCTCGCGGAAAGCCTTTAATACATACATGGCCACCACGAGGTTAACAACCACAACAGCCGTGAGAACTGACCAACAGTTGACCGTGAAAAGGTCCAAATGGGGGAAGGACTCCTGCAGCCAGCTGCGGACGCCATAGAAGCCCAAGAAGGGCAGCGTGAACATGAGCACACTGTAGGCCAACAGCCAGAGGAAGACCTCCGCACTGGTGTCCTCCTGCTGCGTATGATGCTC

AGAATCAATGACAGCCGGGGAATCATCAGTGGTAGCTGGCACCACATCGACATGCTGTTGTTTCCTGTTCTTCTTGCCCATCTCAGCCGGCTAATACTGGTAATCGGTGTGATAACCTGCCTTTTCGCTGGACACCGAAATCTCTGATGGAATTTAGCAGTCGATCCTTGTAACTTCGCACCGTTTGCACGGGTTTGCTTTCGCCGTTCAGCTCCTTCCTGCCAAACAGCAGTTCGGTGACTAGAATCTTGGCCAAACTGGGATCAAAACTGGGATTGTCCCTGAGCAGTCCTGTCTCTTCGATGGCCTTTTCCAGTGCCACACGATTTTCGCTAAATTTTTTCAGAACCGTATGCAAAGAGCGTGTACGCTACAAGTGAAGTGCGAGAGGGAAAAGTTATACGCATATTGGTTTGATCTGCGGACGAGAGGTCCAATCCACTTACGGCATGCTTCTCTGCAAATATTAGGGTCTTGATGCACTTCTGCTGCTCCAGTGCCGCCTTCAAAATCTTGGCAGTCGCCCTGTATTGGGTGGGAACTTTTATGGAATGCGGTTTTTTACTCATTGTCGTCACGTGCACGTGTATGTGTTGTTTGCGCTAGAATAGGTGCAACCCCAATCACGACTTTATCGATATATGCTTATCGAATGTCAGGGCTGGAGATAAGCATGTGGCGTTTGAAACAAATCATATTGTTATTCATGTTTTGAAAATATCTACAATTAATGTAAAGGTTGTTCTAATTTTGCCAACGATAACTACAAAACATATATGTTCTATCCAATATATTTACATACAGGGTATCAAATTTACAAATAAATTGGCAGTGTGGCCATACGTTGCGAGCAAAAATTACTGTACTTCAAGCATTGCCAACACAAGAATATTTTCATGGTTAAAAATTTTGGCGCGATAAAGCCGCAGTTTAAAATGTCAAAGTGTGCGCGTTCTTG

 

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