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CRIMIC CR02154

Gene Past1

CG6148

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3R:12697322..12704875	87C6	FBti0213473	91454

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CATTAGTCACACTGCACTTGCTCG	ATCTCCCACCCATGACATCAGTCGAT

Images

Construct Design Sequences

sgRNA sequence	TCCCCCATGCATTCGTTAGTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGCTGACTGGCCAATTATGC	AATGCATGGGGGAAAGCGA	AGTGGGTCGAGCCAAAGTG	TTGCCACCGAGCCGTATATC

 

Amplicon 5'

Amplicon 3'

CGCTGACTGGCCAATTATGCAATGCCTGGCAACGACTATATATAAACCCTATACTATGAGCGAAACATATTTGAGCTACTATATAAACAAAGTGATAAGCACCGAAAACTGATAAGACGAGCGAGCAAAAAGTCGGTGTCAATTTAGCGCTTAGGCCAGCCACACAAACGCACTTTCTGTGGCAAACTCAACATCGAGGTGGAAGAAAATAGCGCCACAAAACCACGTATTTTCTTTCCTATCTCTTGCAAATACTCTGCTACCCAAAATGTGATAGCCAATTGCTTTGTACTTGAAAAATGGGCGCCAGTGGCGGGCTGGGGCGTGGCAAACCGAAAAATTTTACTATTCATATGTGTCAATAATTATCGGCTTGCTTTCGTCATGGGTAGGAACTCAGCCAAGTTGACTGTTTTCTCCCCGCAACGTGATAAGATTCGTGGCCATGATGTAATCATTGGAAAATACCGATAGCTTGGAGCAGTGCAGGGTTCGTTCTAATTGGGCCATGAGTCATCAGAGAGGGGAAAAAAGAAAATAATAGCAGGCGTACAGTTGGGCATGATAAATATTTCACACTCGAGTACCCAGGCTGAATCGGATCTGGCTCTCCGGAGCAGACGCATCCAGGCAACCATGACGAATACCACGCACTCGATGCCGAGCCAAAACGCCCAGCAATATAATCGCATTTAAGCGTTGAGCATTCGAGGAGGAGCAGTAGGAGGTGGTAGTAGTAACTCAGTTAATCAGAGTCTAGAGGCGCCAGTCGCATTGCAGTCGGAAAAAAGTAAACCCTAATTGAAAGTCTAATTCGATTCGATGCAAGCTGGCTGCTCCACCGGTTCGCCCTGTTACTGTGACCTCCGACCTTCGTGCGTGGCCCAGAGTTCAAGGCCCAGTGCCGCGCACCTGTTCGCTTTCCCCCATGCATT

AGTGGGTCGAGCCAAAGTGGCCTGTTTGTCAACCCTTATCGGGTTGCTAGGCAACTTCTAGTTGGCCGTACTGCACGTTACCCTAGATAAAAATCAATGTAAGGCCGAGTCAGCATGCCATCATCAAATCATGTCTTATCAGTCGACAAAAGTCTGGGCATGTTCCTCTACTAGCACTGCTCTAATTTCAAGCATCAAGTATGCTCATTTAGTTTGTTTATCTGCTCTTACGCTGTTTAGCGTAATTAGGCAACAAGTTGCGGCTACTGGCCGTGCAATTTTTGGCATATTTCAAACAATAAAAGCCGAAATTATCAGAACTAGTGCATATGTATATTATGAGGGGCTCAAAGGTTGTCCACCGTAAAACTTGTAATTATATTCAGCTTTAGGGATCATTGCTTGCTGAGCAAGCAACTTATATGGAAAGCCAGTAACTAATTTGATTGCTCATTTATGTAATCATCCGTTTTGCATGCACAAACCTTTTTTTATCGTGCGAGTAGAAGGCACTGTCAGCATTCTCTTGCCATTTATCAATGCTCCTTATCATGCCAGCTAATGGTGTTGTGCCCAATGAAACACTATAAAACTACAACGGAAAGGCGTGTGATTGGATTAATTCAGTATACAAAGCCGCCAGACAAGGAGAACGAAAATAATTATCTTCCAATATGCCAGGCAACTTAGAATTGGAATAGTAAATATACATATGTATATATACATTCCAATATGTGCTGCCTCCGAGGAGGAGAGATTGTCGTCCAGCTGATCAAACAGCACCCAGAATAATCCCCACGCCCAGTTCGCCCAGACGATCCCTGGTCAACCCATCGCCACAACCCACCTCTGGCGATTATATACATATGTACGTATATATGATCTGTAAACCGAACCGGTTGCAGTCGCATACGAGTACATGTATTGCTGGCGTTGGCAACCTGAGGCCGCGTATCAATTTGATGCTCTCCGATTGTGACTTGGGGCGTGTGGCACTAAGTGGCTCATCCTCCGTCTAATCCGCTCGTGAATTCCACGAAGGGGCCCACGGAATTCAGATGAACTAGCGCTAAGTGCAGGAAGAAAGCATTTTACTCTATGATATACGGCTCGGTGGCAA

 

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