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CRIMIC CR02156

Gene Pp1alpha-96A

CG6593

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3R:24518270..24522331	96A5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCACCGCAACAAACTGAAACTGGT	TTGAAGAGAGTGAACTCCGAGCTGAC

Images

Construct Design Sequences

sgRNA sequence	TGTGGGGAATGGGTGATGATGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTCGAAATCGCATCCGCAAG	GGTGGGGGCAAGGTAATCAA	ATCACCCATTCCCCACATGC	GATGCGTCGTATCTGCTCCA

 

Amplicon 5'

Amplicon 3'

CTCGAAATCGCATCCGCAAGTTTGTCGAAAAACTGTGCTCCGCCAAGTTCTCGGAACGCGCGGAAATGTTGCTGAAGCACAAGAGTCCACTCAAGCCAATGGAGGTTCCAGGCAAAGGGATCCCGAGGTCTGCGATCTCTGAAAAGTCTGAAGAGGATATAGCCAGCTCTCAAGGCGAAGAGCAGACGGATGAGCAAACGGATAGCAGCGAACAGCAAACTCGAAGCCCCAGTCCTCAAAGACTAGTTAGTCGCGATTTCAAGGCTCGTCTGGAGGAGATCCTGGTCAAGCCGGCGGCCACTATTCGAGGAGCTTCCAAGTCGTCCCTGAGTTCGAGGCCAGTTCCGCTGCCCAGGAAGCGGGTGATGTTCAACACCACGGAGGACGGGAAGAGCTTCAATGACAGCGATGATAACCTAAAATGAGTCAATAAAGATGATATTATAATACTTTGGGTATAAATGTCAACGATACGTTTAATAGCCAATATATCATAAGATAATTATATTCCTTTATGAGTACCTATCACCTGTTCAATGTACCGTACTTAACTAAAAAATATTGTTTTTTAAATGAATTTGCATATTCCGGTCACACTACCTTTTACCGATAGCTGCGCGCCGTGCAATGGATAGGCAATCGATAGGCAATCGACTCGCGCTGTAAACAATCGACGTCACTAACCAACGTAGATGAAAATTTTTTAGTACGTCGCGAGTAAAACGTGCATTAAATGCAATAAAAGGGCAGCTGGCTAAGGATCGGAACGATTGAAGTCAGCCAACCTCAGAGGGGTGAGCAACCGAGGCAGTGAATCCGCGAATCCGTGCATCCAGCTGACCAAAGGGCGAGATTAGCCCAGCACCGAGTTTCCATCCGCACACGGACACGGCGCACACACACACAGGCACACGCAGCCCGTGCCCATCGATGAAATAACATACATAACGAACGTATACCGAAAATACACGGGTTACGGGAAATAGCAGTTGCTGCAATCGGCGGATAAGCGAACTTCAGGAAGCTGAGCAAGCGGGCCCACAAAGATGTCGGATATCATGAACATCGACAGTATCATCTCGCGCCTGCTGGAGGGTAGGTGTTAGGGCTCCAACTTTTGATTACCTTGCCCCCACC

ATCACCCATTCCCCACATGCCTTGCCACCCAACCACCGCCCACTCTCATCACCTTAACGTAAATTTTCCCTGCTTGGTTTTCCATTGCAGTGCGCGGTGCACGACCGGGAAAGAATGTTCAGCTATCAGAGAGCGAGATCCGCAGCCTGTGCCTCAAGTCCCGCGAGATATTCCTGTCGCAGCCCATACTCCTGGAGCTGGAGGCACCGCTGAAGATCTGCGGCGACATACACGGACAATATTATGACCTCTTGCGCCTGTTCGAATACGGCGGCTTTCCGCCCGAGTCAAACTACCTGTTCCTGGGCGACTATGTCGATCGCGGCAAGCAATCCCTGGAGACGATATGCCTGCTGCTGGCCTACAAGATCAAGTACGCGGAGAACTTCTTCTTGCTGCGCGGCAACCATGAGTGCGCCAGCATCAATCGCATCTACGGGTAAGTGGGGAGGCGGCTCCAAAAACAGGATGAAGCTTGATGAAATGGTTAGCAATCTGTTCAGTTCAGCACCGAAACAATTTGCTTTCCTGCTTAGATTACTGAAACTCATTTGATTACAATTTATATTATTCTTACGACTCGTATTTACTTCTGATGACTATGTACTTTTATACATGCTATAGAACTTATAGTTGTTTATTGTTTACGTGAGCTTTAAAAATTTCCCAGACATATTGATGTCTACTTATGCTTCCAAGCTTATTAATACTTTCTAGTTTATTGACGCACACAACGATATCCATTACAATTAAATTAGCCATACTTCAAATACATAAAGTAAATGCCACATTCTATAGCAATAATCCATAAAATTACTCAACTAATTGCACTTAATTGGCAGGTTCTATGACGAATGTAAACGTCGCTACACCATCAAGCTGTGGAAAACTTTCACGGACTGCTTCAACTGCCTGCCGGTGGCCGCCATCGTGGATGAGAAGATCTTCTGCTGCCACGGTGGCCTGAGTCCAGATCTGTCGTCCATGGAGCAGATACGACGCATC

 

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