CR02160

CRIMIC CR02160

Gene sud1

CG44254

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3R:24915840..24917864	96B6	FBti0217700	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTGATGCAGCGAAGTGTAGCCATTCA	ATCCAGATCGGTGAAATCTCGCAGCA

Images

Construct Design Sequences

sgRNA sequence	ACTCACACACGCGTTATCAAAGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTCATTGGTATCGCCGTGGA	ATAACGCGTGTGTGAGTGCT	AGGCAAAATCAAAGGCACCG	CCCATTGCTCCTCCGTATCC

Amplicon 5'	Amplicon 3'
CTCATTGGTATCGCCGTGGAACCATCCGTTTATGGTAAGACGCGGGTAGTCGAATGTAGTAACCTCGCCTACTTGGTGGAAGGAGCGCGAGCCCACCTTGAAGAAGGCAAACTGGTTGTCTTTGGGGGCGATCTTTCGCTGCACTGGAAACTGTGGAAAGCACTGATCGTCGCTACCGAAGATCTCTAGGCATCCGCCCTGCTCATCCGTCCATTCCTCCGCTCCTTCCCAAGGCGATAGATAGTAAATGAAGGCCACTTGTCGGTCCTTAAGCAGATCGTCGTGCACGAGCAGATAGTCGCCGCAGGTGTACATGCTGCAGGAGGCGGACACATAGTCCAGCTTGAGGTTGGTAACCTTCTCCAGCCAGGGACGCACTTGTTTGCGCAGCACCTGCAGGAAGTTGGTCAACAGTCGGCAAGCGGGCATGTTGGAGAGGTCGGCGCTCTGGTAGAACTCATACAAATCCATCTGTTTGCGCGACCACTGCACCTTCTGGATGATCTCGGCGACCAGCGCCTGGCTTTGGCCCTTCTCCAGCATGCCGGGCAGAAGGCAGATTTGAAAGGGATCCGAGAAGACCTGAGCACCGGTTTGCGTCTCCTTGGTCCCTTGGCTCTTCTCCTCCGTCCAGGCTGTTTGGAGCCACTTGGTAAGTTCCGGTTGCTGATACGCCTCGTTCAGGAGCACTTCGTTCGTTTCAAGGATGTCGCCCAGGCGCAAAAGCTTCCTGTGCGGCTCGCCCACCTGATCTGCGGAATCCTCCTGCTCTGCACGACCATCTGCAAGGGCAACATAGTGAATGGGTTTGCATTAAGCGAGGCAAACAGGGGCATACCTTAAACTTTAATTTGGCTATTTTTTACGCACACCAGCACTCACACACGCGTTAT	AGGCAAAATCAAAGGCACCGATGTGCCGGCACACCTCGCTTTATCGTTTTGGGGCAGATTTCTCACTGTTTCCACTTACCTTCGTCCTTGTCTTTTCTCCTGGGCTTTACTGGACTGGAGCTCGAGGTTTCCATGATGCTGTGAGTCAAATTTTCGGTTAAAAACGTGAATTTTTGCCAGGGAAAATAAAAAAATTGGCTTGTGCAGCTAGAGATGGTAGCTCGGAAATATCGGTTGTTCTGTGCAAGTGTTGGGTAGTTAGCTTATTCCACGCAGTATCAAATACCTAGCTAAAGTTTAGTAGCTAAAAACTTTTGGCATTATATTGTAAACAATAAAGGGCGTATATAAGTAATAATATATAATATAATAATATATTGAAGGCATTCAGCATTAGCATTCAACTCACTAATCACACTTTCATTTTATACTGATCATCACTGATTGTACGTGAGTGTCGTGTGTTTCGTCGTCGCCTCACGCCTCCATCCCTAGCCAATTAGTAACGAACAATTGCTCGATCGGCTCCACGTAAATAAAGTTTAGATCACAGCTTTGTTGATTGCTTATCGCGGTGGCAAATATAGTTATTCGTTTATGGCTGTAAACTTTCGAAACGGACGTGGACATAGCCGGTAAATTAAAACTTGAAGATAAAACCTAGCTGGTGACAAAGAAATTCCAATTGGAGCAGTCCAATGCCGTGGAATGACCTTGGAAGAGGCGGAGCCACCATTCAGCCCTCCATATTATAAACAAAAATACTATTGTGGAATGTTCACAGCTATTTTGTATAAAAAAAATATTTGGCTATCAGTCTGTGCGTGCTAGGGCCAAACAGCTGATTCCGCCTGGCTGCCACCTGATTTGTTGGGGGTGTGAGTGGGGCTGGGGCTGTGGTATTCTGTATGCTCGCGATGATCTCATTGTGTTGGAATCGGAGCAAAAGCTAAACTAAACAACTTGACATGTGTTCTTCTGCTCCTGTGTAGCTACCTAGTGTTGGAGGATACGGAGGAGCAATGGG

Amplicon 5'

Amplicon 3'

CTCATTGGTATCGCCGTGGAACCATCCGTTTATGGTAAGACGCGGGTAGTCGAATGTAGTAACCTCGCCTACTTGGTGGAAGGAGCGCGAGCCCACCTTGAAGAAGGCAAACTGGTTGTCTTTGGGGGCGATCTTTCGCTGCACTGGAAACTGTGGAAAGCACTGATCGTCGCTACCGAAGATCTCTAGGCATCCGCCCTGCTCATCCGTCCATTCCTCCGCTCCTTCCCAAGGCGATAGATAGTAAATGAAGGCCACTTGTCGGTCCTTAAGCAGATCGTCGTGCACGAGCAGATAGTCGCCGCAGGTGTACATGCTGCAGGAGGCGGACACATAGTCCAGCTTGAGGTTGGTAACCTTCTCCAGCCAGGGACGCACTTGTTTGCGCAGCACCTGCAGGAAGTTGGTCAACAGTCGGCAAGCGGGCATGTTGGAGAGGTCGGCGCTCTGGTAGAACTCATACAAATCCATCTGTTTGCGCGACCACTGCACCTTCTGGATGATCTCGGCGACCAGCGCCTGGCTTTGGCCCTTCTCCAGCATGCCGGGCAGAAGGCAGATTTGAAAGGGATCCGAGAAGACCTGAGCACCGGTTTGCGTCTCCTTGGTCCCTTGGCTCTTCTCCTCCGTCCAGGCTGTTTGGAGCCACTTGGTAAGTTCCGGTTGCTGATACGCCTCGTTCAGGAGCACTTCGTTCGTTTCAAGGATGTCGCCCAGGCGCAAAAGCTTCCTGTGCGGCTCGCCCACCTGATCTGCGGAATCCTCCTGCTCTGCACGACCATCTGCAAGGGCAACATAGTGAATGGGTTTGCATTAAGCGAGGCAAACAGGGGCATACCTTAAACTTTAATTTGGCTATTTTTTACGCACACCAGCACTCACACACGCGTTAT

AGGCAAAATCAAAGGCACCGATGTGCCGGCACACCTCGCTTTATCGTTTTGGGGCAGATTTCTCACTGTTTCCACTTACCTTCGTCCTTGTCTTTTCTCCTGGGCTTTACTGGACTGGAGCTCGAGGTTTCCATGATGCTGTGAGTCAAATTTTCGGTTAAAAACGTGAATTTTTGCCAGGGAAAATAAAAAAATTGGCTTGTGCAGCTAGAGATGGTAGCTCGGAAATATCGGTTGTTCTGTGCAAGTGTTGGGTAGTTAGCTTATTCCACGCAGTATCAAATACCTAGCTAAAGTTTAGTAGCTAAAAACTTTTGGCATTATATTGTAAACAATAAAGGGCGTATATAAGTAATAATATATAATATAATAATATATTGAAGGCATTCAGCATTAGCATTCAACTCACTAATCACACTTTCATTTTATACTGATCATCACTGATTGTACGTGAGTGTCGTGTGTTTCGTCGTCGCCTCACGCCTCCATCCCTAGCCAATTAGTAACGAACAATTGCTCGATCGGCTCCACGTAAATAAAGTTTAGATCACAGCTTTGTTGATTGCTTATCGCGGTGGCAAATATAGTTATTCGTTTATGGCTGTAAACTTTCGAAACGGACGTGGACATAGCCGGTAAATTAAAACTTGAAGATAAAACCTAGCTGGTGACAAAGAAATTCCAATTGGAGCAGTCCAATGCCGTGGAATGACCTTGGAAGAGGCGGAGCCACCATTCAGCCCTCCATATTATAAACAAAAATACTATTGTGGAATGTTCACAGCTATTTTGTATAAAAAAAATATTTGGCTATCAGTCTGTGCGTGCTAGGGCCAAACAGCTGATTCCGCCTGGCTGCCACCTGATTTGTTGGGGGTGTGAGTGGGGCTGGGGCTGTGGTATTCTGTATGCTCGCGATGATCTCATTGTGTTGGAATCGGAGCAAAAGCTAAACTAAACAACTTGACATGTGTTCTTCTGCTCCTGTGTAGCTACCTAGTGTTGGAGGATACGGAGGAGCAATGGG

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